Appraisal of RAPD and ISSR markers for genetic diversity analysis among cowpea (Vigna unguiculata L.) genotypes

2014 ◽  
Vol 17 (2) ◽  
pp. 79-88 ◽  
Author(s):  
Harsukh Popatbhai Gajera ◽  
Rinkal Kishorbhai Domadiya ◽  
Sunil Vrajlal Patel ◽  
Baljibhai Arjanbhai Golakiya
2021 ◽  
Vol 50 (1) ◽  
pp. 119-129
Author(s):  
PN Narasimha Murthy ◽  
NB Patel ◽  
Chintan Kapadia ◽  
KD Desai ◽  
G Koteswara Rao

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.


2020 ◽  
Vol 23 ◽  
pp. 101495
Author(s):  
G. Koteswara Rao ◽  
Chintan Kapadia ◽  
N.B. Patel ◽  
K.D. Desai ◽  
P.N. Narasimha Murthy

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (3) ◽  
pp. 265-274 ◽  
Author(s):  
Sangeeta Srivastava ◽  
Prashant S. Gupta ◽  
Vimal Kumar Saxena ◽  
Hari Mohan Srivastava

2014 ◽  
Vol 10 (8) ◽  
pp. 479-486 ◽  
Author(s):  
Chandra Sekhar Singh Bhaludra ◽  
Hari Yadla ◽  
Farhan Sachal Cyprian ◽  
Rama Rao Bethapudi ◽  
S.D. Basha ◽  
...  

Author(s):  
Mudasir A. Mir ◽  
Sheikh Mansoor ◽  
M. Sugapriya ◽  
Mohammed Nasser Alyemeni ◽  
Leonard Wijaya ◽  
...  

2017 ◽  
Vol 6 (11) ◽  
pp. 1775 ◽  
Author(s):  
Kokkanti Mallikarjuna ◽  
Chandra Kala Sirigiri ◽  
Ratnakar Reddi K.V.N. ◽  
Chandra Sekhar Akila ◽  
Obul Reddy Puli Chandra

Canthium parviflorum Lam is an important medicinal plant widely used in traditional systems of medicine with propagation limitations. In the present work, we are reporting the genetic diversity analysis of naturally occurring and in vitro grown plants by RAPD and ISSR markers. The plants developed on MS medium supplemented with BA (2mg/l) and NAA (0.5mg/l) using nodal and leaf explants were used along with plants present in five different geographical areas. Genetic diversity analysis using DNA based markers, RAPD and ISSR indicated that considerable genetic variations are present in naturally occurring plants. It is also indicated that tissue culture plants and their wild relatives show genetic similarity by grouping into one clad. The amplification products of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. The variations observed in naturally occurring plants could be due to the impact of local environmental factors and accumulation of mutations in the course of evolution. This is the first report on genetic diversity of Canthium plant populations.


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