Efficient procedures for callus induction and adventitious shoot organogenesis in sugar beet (Beta vulgaris L.) breeding lines

2004 ◽  
Vol 40 (5) ◽  
pp. 475-481 ◽  
Author(s):  
Chun-Lai Zhang ◽  
Dong-Fang Chen ◽  
Malcolm C. Elliott ◽  
Adrian Slater
2013 ◽  
Vol 12 (4) ◽  
pp. 168-178 ◽  
Author(s):  
Farhad Taghipour ◽  
Narges Janalizade ◽  
Maryam Eshrati ◽  
Taraneh Hassanzade ◽  
Fahrul Huyop

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 211-213 ◽  
Author(s):  
Michael E. Compton ◽  
D.J. Gray

Cotyledon explants of four watermelon [Citrullus lanatus (Thunb.) Mataum. & Nakai] breeding lines (F92U8, SP90-1, SP90-2, and SP90-4) were prepared from mature seed or from 2-, 4-, 6-, 8-, or 10-day-old seedlings. Explants were incubated on shoot regeneration medium for 8 weeks followed by 4 weeks on shoot elongation medium. The four genotypes differed in their ability to produce shoots at each explant age. The highest frequency with which F92U8 (66%) and SP90-2 (60%) explants produced shoots was for 2-day-old seedlings. Fewer explants formed shoots when established from mature seed or seedlings older than 2 days. In contrast, the percentage of SP90-4 explants that produced shoots was highest when cotyledons were obtained from 4-day-old seedlings (40%), but the response was less than the optimum for F92U8 and SP90-2. SP90-1 cotyledon explants exhibited the poorest response of the four breeding lines (<11% produced shoots), with little difference in response among the explant ages tested. The number of shoots per responding explant also depended on the age of the explant source. Explants from 2- to 4-day-old seedlings produced the most shoots. Fewer shoots formed on cotyledons from mature seed or seedlings older than 4 days.


2020 ◽  
Vol 270 ◽  
pp. 109416 ◽  
Author(s):  
Claudia Maria Liberatore ◽  
Margherita Rodolfi ◽  
Deborah Beghè ◽  
Andrea Fabbri ◽  
Tommaso Ganino ◽  
...  

1999 ◽  
Vol 124 (6) ◽  
pp. 630-635 ◽  
Author(s):  
Min Wang ◽  
Irwin L. Goldman

Genetic relationships among 37 accessions of Beta vulgaris, including 21 table beet, 14 sugar beet, and two Swiss chard (Beta vulgaris ssp. cicla) accessions, were evaluated using randomly amplified polymorphic DNA (RAPD). Genetic distance was estimated based on the presence or absence of polymorphic RAPD bands. Multidimensional scaling plots of genetic distance values revealed that table beet inbred lines from the University of Wisconsin Table beet Breeding Program clustered in an intermediate position between sugar beet breeding lines and standard table beet germplasm, likely because of their origin from an introgression program designed to incorporate sugar beet genes.


2008 ◽  
Vol 93 (2) ◽  
pp. 209-221 ◽  
Author(s):  
Chun-Lai Zhang ◽  
Dong-Fang Chen ◽  
Marie Kubalakova ◽  
Jian Zhang ◽  
Nigel W. Scott ◽  
...  

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873E-873
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp

Dissected white ash seeds were placed on an agar-solidified MS medium with 10 μM TDZ and 1 μM 2,4-D (shoot initiation medium). After 4 weeks, explants were transferred to shoot elongation medium (3 μM TDZ, 1 μM BA, and 1 μM IBA) solidified with 0.7% Sigma agar, 0.525% agar + 0.05% gelrite, 0.35% agar + 0.1% gelrite, 0.175% agar + 0.15% gelrite, 0.2% gelrite, or no gelling agent (liquid medium). After 12 weeks in vitro, shoot growth and number were suppressed in cultures containing 0.2% gelrite (9.3 mm and 0.7 shoots) and in cultures containing no gelling agent (6.9 mm and 0.7 shoots). There were no differences in shoot growth and number in cultures containing 0.7% Sigma agar (2.2 mm and 16.5 shoots), 0.525% agar + 0.05% gelrite (2.6 mm and 18.7 shoots), 0.35% agar + 0.1% gelrite (1.6 mm and 17.4 shoots), and 0.175% agar + 0.15% gelrite (2.0 mm and 20.4 shoots). The most vitrification occurred in cultures on medium with the lowest amount of agar, gelrite only, and liquid medium.


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