scholarly journals HIGH-FREQUENCY INDUCTION OF MULTIPLE SHOOTS AND PLANT REGENERATION FROM COTYLEDONARY NODE EXPLANTS OF TONGKAT ALI (EURYCOMA LONGIFOLIA JACK)

2020 ◽  
Vol 18 (5) ◽  
pp. 6321-6333
Author(s):  
A.G.A. ALTTAHER ◽  
Z.N.B. YUSOF ◽  
M. MAHMOOD ◽  
N.A. SHAHARUDDIN
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Multiple Shoots ◽  
Cotyledonary Node ◽  
Eurycoma Longifolia ◽  
Eurycoma Longifolia Jack ◽  
Cotyledonary Node Explants ◽  
High Frequency Induction

scholarly journals Tissue Culture and Plant Regeneration of Cowpea (Vigna unguiculata)

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 627f-627
Author(s):  
Lurline Marsh
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Regeneration ◽  
Vigna Unguiculata ◽  
Nutrient Medium ◽  
Cotyledonary Node ◽  
Naphthalene Acetic Acid ◽  
Leaf Segments ◽  
Dichlorophenoxy Acetic Acid ◽  
Cotyledonary Node Explants

Explants (cotyledon, cotyledonary node, second node, hypocotyl, epicotyl, and leaf) of cowpea (Vigna unguiculata) genotypes MN13 and Pinkeye Purple Hull were cultured on Murashige and Skoog basal nutrient medium. The medium was supplemented with 1 mg·L–1 benzyladenine (BA) or 1 mg·L–1 benzyladenine plus naphthalene acetic acid (BA + NAA) or 2 mg·L–1 2,4-dichlorophenoxy acetic acid (2,4-D). Cultures were maintained at 22°C for 1 month, after which they were transferred to 1 mg·L–1 BA + NAA. Cotyledons, hypocotyl, epicotyl, and leaf segments produced only calli after subculturing in BA + NAA. The second node and cotyledonary node explants cultured on the BA or BA + NAA followed by subculture on BA + NAA produced calli, shoots, and roots. The plants were then transplanted to promix but later died.

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