Several studies have pointed out the promising use of nutritional diagnosis methods for the determination of optimum nutrient contents in plant tissues. The present investigation was carried out in different oases in Southern Tunisia to determine reference values for the interpretation of leaf analyses of date palm (Phoenix dactylifera) Deglet Nour cultivar with the Critical Value Approach (CVA) and the Compositional Nutrient Diagnosis (CND). A database (n = 100) of yield and mineral concentrations taken from date palm leaflets in October, at the maturity stage of dates, was used. The yield cut-off between low-yield and high-yield subpopulations, selected from cumulative variance ratio functions across survey data, was 76 kg palm−1 and the global nutrient imbalance index (CNDr2) was 10.06. Critical CND nutrient indices were found to be symmetrical around zero as follows: (1.59; +1.59) for IN, (−0.44, +0.44) for IP, (−0.63, +0.63) for IK, (−0.94, +0.94) for ICa, (−1.05, +1.05) for IMg, (−0.80, +0.80) for IFe, (−0.74, +0.74) for ICu, (−0.80, +0.80) for IB, (−0.93, +0.93) for IZn, (−1.04, +1.04) for IMn, and (−1.03, +1.03) for the residual value. Compared to CND, the CVA approach shows weak detection of the nutrients that cause nutritional imbalance. CND indices revealed, except for N, the presence of nutrient imbalances and the necessity to correct the mineral nutrition of date palm in the Kebeli oases.
Determination of genome size variations among different date palm cultivars (Phoenix dactylifera L.) by flow cytometry
