Optimization of parameters to improve transformation efficiency of elephant foot yam (Amorphophallus paeoniifolius (Dennst.) Nicolson

3 Biotech ◽  
2021 ◽  
Vol 11 (6) ◽  
Author(s):  
Leen N. Abraham ◽  
S. Kamala ◽  
J. Sreekumar ◽  
T. Makeshkumar
Keyword(s):  
Transformation Efficiency ◽  
Optimization Of Parameters ◽  
Elephant Foot Yam

scholarly journals Optimization of Parameters for Particle Bombardment of Genes to Garlic

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 616c-616
Author(s):  
J. Michele Myers ◽  
Philipp W. Simon
Keyword(s):  
Particle Bombardment ◽  
Promoter Activity ◽  
Transformation Efficiency ◽  
35S Promoter ◽  
Cell Type ◽  
Vacuum Filtration ◽  
Optimization Of Parameters ◽  
Camv 35S ◽  
Gus Reporter ◽  
Enhance Promoter Activity

We evaluated the efficiency of transformation in garlic for promoter activity, osmoticum effect and shaker speed using particle bombardment as the method of gene delivery. Callus was produced from root segments on a modified B-5 medium for four garlic clones. Suspension cultures were then established on a modified B-5 medium + 2,4-D using 6-month-old callus. Cells were collected by vacuum filtration and the Bio-Rad PDS-1000/He system was used to deliver genes. The activities of CaMV 35S, maize Adh1, and rice Act promoters were evaluated for transient expression using the β-glucuronidase (GUS) reporter gene. Osmotic conditioning of cells was performed by adding both mannitol and sorbitol to the medium. Osmoticum effect was evaluated for enhancement of transformation efficiency using GUS. The effect of shaker speed (120, 180 and 240 rpm) on cell type was evaluated for transformation efficiency using GUS. CaMV 35S promoter activity was much higher for garlic than either the maize Adh1 or rice Act promoters. Osmoticum did not enhance promoter activity, but differences in response to osmoticum among garlic clones were observed. Shaker speed did affect cell type, and transformation efficiency was greatly increased at higher shaker speeds. Confirmation of stable transformation and regeneration are in progress.

Optimization of Parameters of RF Pulse Sequences for MRI Investigations in the Presence of Magnetic Nanoparticles

2020 ◽  
Vol 65 (9) ◽  
pp. 1416-1420
Author(s):  
Yu. V. Bogachev ◽  
A. V. Nikitina ◽  
V. V. Frolov ◽  
Ya. Yu. Marchenko ◽  
B. P. Nikolaev
Keyword(s):  
Magnetic Nanoparticles ◽  
Pulse Sequences ◽  
Optimization Of Parameters ◽  
Rf Pulse

scholarly journals Optimizing Transformation Frequency of Cryptococcus neoformans and Cryptococcus gattii Using Agrobacterium tumefaciens

2021 ◽  
Vol 7 (7) ◽  
pp. 520
Author(s):  
Jianmin Fu ◽  
Nohelli E. Brockman ◽  
Brian L. Wickes
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Yeast Cell ◽  
Transformation Efficiency ◽  
Transformation Frequency ◽  
Yeast Cells ◽  
Agar Concentration ◽  
Genetic Tool ◽  
Number Of Factors ◽  
Cryptococcus Spp ◽  
Transformation Systems

The transformation of Cryptococcus spp. by Agrobacterium tumefaciens has proven to be a useful genetic tool. A number of factors affect transformation frequency. These factors include acetosyringone concentration, bacterial cell to yeast cell ratio, cell wall damage, and agar concentration. Agar concentration was found to have a significant effect on the transformant number as transformants increased with agar concentration across all four serotypes. When infection time points were tested, higher agar concentrations were found to result in an earlier transfer of the Ti-plasmid to the yeast cell, with the earliest transformant appearing two h after A. tumefaciens contact with yeast cells. These results demonstrate that A. tumefaciens transformation efficiency can be affected by a variety of factors and continued investigation of these factors can lead to improvements in specific A. tumefaciens/fungus transformation systems.

scholarly journals WUSCHEL Overexpression Promotes Callogenesis and Somatic Embryogenesis in Medicago truncatula Gaertn

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 715
Author(s):  
Aline Kadri ◽  
Ghislaine Grenier De March ◽  
François Guerineau ◽  
Viviane Cosson ◽  
Pascal Ratet
Keyword(s):  
Somatic Embryogenesis ◽  
Medicago Truncatula ◽  
Transformation Efficiency ◽  
Genetic Manipulation ◽  
Leaf Explants ◽  
Promising Tool ◽  
Limiting Step ◽  
Wus Gene ◽  
Multiple Signaling ◽  
Regeneration Systems

The induction of plant somatic embryogenesis is often a limiting step for plant multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones and the induction of specific genes. The WUSCHEL gene (WUS) is required for the production of plant embryogenic stem cells. To explore a different approach to induce somatic embryogenesis, we have investigated the effect of the heterologous ArabidopsisWUS gene overexpression under the control of the jasmonate responsive vsp1 promoter on the morphogenic responses of Medicago truncatula explants. WUS expression in leaf explants increased callogenesis and embryogenesis in the absence of growth regulators. Similarly, WUS expression enhanced the embryogenic potential of hairy root fragments. The WUS gene represents thus a promising tool to develop plant growth regulator-free regeneration systems or to improve regeneration and transformation efficiency in recalcitrant crops.

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