Consistency evaluation of lung adenocarcinoma tissue and circulating tumor cells related gene mutation detection based on multi-site immunomagnetic beads

This study was designed to investigate the feasibility of genetic testing using circulating tumor cells (CTCs) instead of tumor tissues in lung adenocarcinoma to break through its limitation. Separation system for epithelial cell adhesion molecule (EpCAM), epidermal growth factor receptor (EGFR), and Vimentin expressing CTCs was constructed and used to capture CTCs in the blood samples of 57 patients with lung adenocarcinoma. Genetic mutations of genes involved in targeted therapies were detected by next-generation sequencing (NGS) in tissues from these patients. Blood CTC samples with the gene mutations identified by tissue-NGS were selected and corresponding gene mutations were detected by Sanger sequencing. The specificity of the CTC separation system was 95.48% and the sensitivity was 90.85%. The average number of CTCs in the blood of patients with lung adenocarcinoma was 12.47/7.5 mL. Comparison of the tissue-NGS with the CTC-Sanger sequencing showed that the consistencies of genetic mutations of EGFR ( n = 24), KRAS ( n = 9), TP53 ( n = 19), BRAF ( n = 1), ERBB2 ( n = 2), and PIK3CA ( n = 3) were 92.31%, 75.00%, 86.36%, 50.00%, 66.67%, and 75.00%, with an overall consistency of 84.06%. The CTC separation system established in this study shows high specificity and sensitivity. CTCs can be used as a suitable alternative to tumor tissues that are difficult to obtain in clinical practice and overcome the difficulties in tumor tissue collection, which is of significance in guiding clinical medication and individualized treatment with significant clinical application value in terms of genetic testing for targeted therapies in tumor treatment.

Mag-spinner: A next-generation Facile, Affordable, Simple, and porTable (FAST) magnetic separation system

Mag-spinner, a system in which magnets are combined with a spinner system, is a new type of magnetic separation system for the preprocessing of biological and medical samples. Interference by...

Selective crystallization of dysprosium complex from neodymium/dysprosium mixture enabled by cooperation of coordination and crystallization

Designing a molecular-level Ln3+ separation system remains a challenge for developing next-generation separation methodologies. Herein, we report crystallization-based Nd3+/Dy3+ separation using a tripodal Schiff base ligand. Highly selective crystallization of...

IMPROVEMENT OF TECHNICAL MEANS OF CONTROL SYSTEMS AND PROTECTION OF THE MARINE ENVIRONMENT FROM OIL POLLUTION

Data on environmental monitoring and the marine environment pollution by oil are presented and new highly efficient technical means of oil-containing water purification systems are developed, which have high quality of oil-containing water purification, a long service life of filter elements, simple design and operation. The combined separation system for the purification of oily waters has a high efficiency and provides the purification of oily waters to a concentration of petroleum products lower than 5 million-1. The design of the regenerated filter and separator with granular loading ensures the regeneration of filter elements without disassembling and replacing them, which significantly simplifies the maintenance and operation of the system.

Development of an Online Detection Setup for Dissolved Gas in Transformer Insulating Oil

The type and concentration of dissolved gases in transformer insulating oil are used to assess transformer conditions. In this paper, an online detection setup for measuring the concentration of multicomponent gases dissolved in transformer insulating oil is developed, which consists of an oil-gas separation system and an optical system for acquiring the transformer status in real time. The oil-gas separation system uses low pressure, constant temperature, and low-frequency stirring as working conditions for degassing large-volume oil samples based on modified headspace degassing. The optical system uses tunable diode laser absorption spectroscopy (TDLAS) to determine the gas concentration. Six target gases (methane, ethylene, ethane, acetylene, carbon monoxide, and carbon dioxide) were detected by three near-infrared lasers (1569, 1684, and 1532 nm). The stability of the optical system was improved by the common optical path formed by time-division multiplexing (TDM) technology. The calibration experiments show that the second harmonics and the concentrations of the six gases are linear. A comparison experiment with gas chromatography (GC) demonstrates that the error of acetylene reaches the nL/L level, while the other gases reach the μL/L level. The data conforms to the power industry testing standards, and the state of the transformer is analyzed by the detected six characteristic gases. The setup provides an effective basis for the online detection of dissolved gas in transformer insulating oil.

OPTIMAL SOLUTION OF LINEAR EQUATION SYSTEM FOR RADIO SYSTEMS WITH MULTIPOLARIZATION MULTIPLEXING

Приводятся результаты исследования возможности увеличения эффективности использования радиочастотного спектра более чем в два раза за счет использования нескольких (N>2)радиосигналов с неортогональными поляризациями,передаваемых на общей несущей частоте. Предложены метод выявления такой возможности для заданной комбинации поляризаций радиосигналов, а также структурная схема и процедура определения параметров устройства поляризационного разделения радиосигналов на приемной стороне. Выполнен детальный анализ определения оптимальных коэффициентов элементов системы поляризационного разделения на приемной стороне четырех радиосигналов с учетом иррациональности коэффициентов анализируемой системы линейных уравнений. The results of the study of the possibility of increasing the efficiency of using the radio frequency spectrum by more than twice due to several (N>2) non-orthogonal polarizations of carrier radio signals are presented. The method to determine such possibility for any given set of polarizations, as well as a block diagram and a procedure for determining the parameters of a device for polarization separation of radio signals on the receiving side are proposed. The detailed analysis of the determination of optimal coefficients of elements of polarization separation system on receiving side of four radio signals is performed taking into account the irrationality of coefficients of analyzed linear equation system.

Success Story of First Malaysia Flare Tips Decommissioning in Sarawak Offshore Field Platform

The project was for part production enhancement project which to cater for brownfield & greenfield project. To cater to the production (oil) increment for the brownfield project, the existing flare tips and separation system need to be upgraded with higher capacity. The inclusive project was upgrading existing. Part of the scope was decommissioning the existing flare tip and associated system, e.g., ignition panel and ignition pipe. The project will decommission the current flare tips and replaced it with new higher capacity flare tips with Low Pressure (LP) & High Pressure (HP) connection. The existing flare panel was a single-type ignition system. The existing flare tip had LP & HP tip with 8″ inch size; the weight for both tip was estimated at 300 kg. The concept selection was discussed on the suitable method to lifting down the decommissioning flare tip at the offshore platform. There were 2 suitable techniques selected at the initial of the concept selection. One was lifting down the decommissioning flare tip directly from flare boom to vessel. Another method was manual rigging of the flare tips from the flare boom to the lower deck. After several discussions and workshops, it was decided to proceed with manual rigging of the decommissioning flare tip to the safe deck area. The removal of the decommissioning flare tip was performed during turnaround. The total days for the overall activity of the decommissioning & installation of the new flare tip was 3 days, 2 days ahead from planned duration 5 days.

Liquid amine–solid carbamic acid phase-separation system for direct capture of CO2 from air

The phase separation between a liquid amine and the solid carbamic acid exhibited >99% CO2 removal efficiency under a large-scale gas stream of 400 ppm CO2. Isophorone diamine [IPDA; 3-(aminomethyl)-3,5,5-trimethylcyclohexylamine] reacted with CO2 in the CO2/IPDA molar ratio of ≥ 1 even in H2O as a solvent. The captured CO2 was completely desorbed at 333 K because the disolved carbamate ion releases CO2 at low temperature. The reusability of IPDA under CO2 adsorption-and-desorption cycles without degradation, the >95% efficinecy kept for 100 hours under direct air capture condition, and high CO2 capture rate (214 mmol/h for 1 mol amine) suggest that the phase separation system using IPDA is robust and durable for practical use.