Development and validation of a medium for recombinant endo-β-1,4-xylanase production by Kluyveromyces lactis using a statistical experimental design

2011 ◽  
Vol 62 (1) ◽  
pp. 283-292 ◽  
Author(s):  
Siti Fatimah Zaharah Mohamad Fuzi ◽  
Nor Muhammad Mahadi ◽  
Jamaliah Md. Jahim ◽  
Abdul Munir Abd. Murad ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Experimental Design ◽  
Kluyveromyces Lactis ◽  
Statistical Experimental Design ◽  
Xylanase Production ◽  
Development And Validation

Development and Validation of a LC Method for the Determination of Pramipexole Using an Experimental Design

2006 ◽  
Vol 64 (1-2) ◽  
pp. 95-100 ◽  
Author(s):  
G. Srinubabu ◽  
K. Jaganbabu ◽  
B. Sudharani ◽  
K. Venugopal ◽  
G. Girizasankar ◽  
...  
Keyword(s):  
Experimental Design ◽  
Development And Validation

A Statistical Experimental Design to Plastein Synthesis with the Mixture of Soy Protein Isolate(SPI) Hydrolysate and Whey Protein Isolate(WPI) Hydrolysate

2011 ◽  
Vol 236-238 ◽  
pp. 2773-2779
Author(s):  
Ying Cao Xu ◽  
Zhi Biao Feng ◽  
Chun Hong Liu
Keyword(s):  
Experimental Design ◽  
Whey Protein ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Statistical Experimental Design ◽  
Operating Variables ◽  
Enzyme Substrate ◽  
Predicted Values

A statistical experimental design to plastein synthesis which was catalyzed by transglutaminase, using the mixture of soy protein isolate(SPI) hydrolysate and whey protein isolate (WPI) hydrolysate, was investigated. Enzyme/Substrate(E/S:5-25U/g), pH(5-9) and temperature (35-65°C) were selected as major operating variables. To investigate the effects of variables to yield of plastein, the statistical experiment of Box-Behnken design(BBD) and Response Surface methodology(RSM) was employed. Regression analysis showed that the experiment data accorded with the predicted values obtained from quadratic regression equation in BBD with R-Squared of 0.9866 and F-value of 102.51. The optimum results estimated by BBD were as follows: E/S(19.5U/g), pH(6.8), and temperature(50.0°C), gave a maximum plastein yield of 54%. In the present experiment, the preliminary study on plastein functions such as foaming, emulsifying, were showed that plastein had a good biological function.

Chitinase Production in Solid-State Fermentation by Enterobacter sp. NRG4 Using Statistical Experimental Design

2005 ◽  
Vol 51 (4) ◽  
pp. 222-228 ◽  
Author(s):  
Neetu Dahiya ◽  
Rupinder Tewari ◽  
Ram Prakash Tiwari ◽  
Gurinder Singh Hoondal
Keyword(s):  
Solid State ◽  
Experimental Design ◽  
Solid State Fermentation ◽  
Statistical Experimental Design ◽  
Chitinase Production
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