A Statistical Experimental Design to Plastein Synthesis with the Mixture of Soy Protein Isolate(SPI) Hydrolysate and Whey Protein Isolate(WPI) Hydrolysate

2011 ◽  
Vol 236-238 ◽  
pp. 2773-2779
Author(s):  
Ying Cao Xu ◽  
Zhi Biao Feng ◽  
Chun Hong Liu
Keyword(s):  
Experimental Design ◽  
Whey Protein ◽  
Soy Protein ◽  
Soy Protein Isolate ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Statistical Experimental Design ◽  
Operating Variables ◽  
Enzyme Substrate ◽  
Predicted Values

A statistical experimental design to plastein synthesis which was catalyzed by transglutaminase, using the mixture of soy protein isolate(SPI) hydrolysate and whey protein isolate (WPI) hydrolysate, was investigated. Enzyme/Substrate(E/S:5-25U/g), pH(5-9) and temperature (35-65°C) were selected as major operating variables. To investigate the effects of variables to yield of plastein, the statistical experiment of Box-Behnken design(BBD) and Response Surface methodology(RSM) was employed. Regression analysis showed that the experiment data accorded with the predicted values obtained from quadratic regression equation in BBD with R-Squared of 0.9866 and F-value of 102.51. The optimum results estimated by BBD were as follows: E/S(19.5U/g), pH(6.8), and temperature(50.0°C), gave a maximum plastein yield of 54%. In the present experiment, the preliminary study on plastein functions such as foaming, emulsifying, were showed that plastein had a good biological function.

scholarly journals Krill Protein Hydrolysate Provides High Absorption Rate for All Essential Amino Acids—A Randomized Control Cross-Over Trial

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3187
Author(s):  
Rebekka Thøgersen ◽  
Hanne Christine Bertram ◽  
Mathias T. Vangsoe ◽  
Mette Hansen
Keyword(s):  
Amino Acids ◽  
Whey Protein ◽  
Soy Protein ◽  
Protein Hydrolysate ◽  
Soy Protein Isolate ◽  
Area Under The Curve ◽  
Essential Amino Acids ◽  
Whey Protein Isolate ◽  
Protein Isolate ◽  
Young Males

Background: adequate protein intake is essential to humans and, since the global demand for protein-containing foods is increasing, identifying new high-quality protein sources is needed. In this study, we investigated the acute postprandial bioavailability of amino acids (AAs) from a krill protein hydrolysate compared to a soy and a whey protein isolate. Methods: the study was a randomized, placebo-controlled crossover trial including ten healthy young males. On four non-consecutive days, volunteers consumed water or one of three protein-matched supplements: whey protein isolate, soy protein isolate or krill protein hydrolysate. Blood samples were collected prior to and until 180 min after consumption. Serum postprandial AA concentrations were determined using 1H NMR spectroscopy. Hunger and satiety were assessed using visual analogue scales (VAS). Results: whey and krill resulted in significantly higher AA concentrations compared to soy between 20–60 min and 20–40 min after consumption, respectively. Area under the curve (AUC) analyses revealed that whey resulted in the highest postprandial serum concentrations of essential AAs (EAAs) and branched chain AAs (BCAAs), followed by krill and soy, respectively. Conclusions: krill protein hydrolysate increases postprandial serum EAA and BCAA concentrations in a superior manner to soy protein isolate and thus might represent a promising future protein source in human nutrition.

A Statistical Experimental Design to Degradation of Aqueous Methyl Orange Solution with Photocatalyst of Ti-TiO2 Nanotube Array

2012 ◽  
Vol 479-481 ◽  
pp. 221-225
Author(s):  
Ying Cao Xu ◽  
Hong You
Keyword(s):  
Methyl Orange ◽  
Experimental Design ◽  
Uv Light ◽  
Methyl Orange Solution ◽  
Statistical Experimental Design ◽  
Nanotube Array ◽  
Box Behnken Design ◽  
Operating Variables ◽  
Predicted Values ◽  
Orange Solution

A statistical experimental design to the degradation of aqueous methyl orange, by using the Ti/TiO2 nanotube array photocatalyst, under UV irradiation, was investigated. Concentration of methyl orange(5mg/L,15mg/L,25mg/L), catalytic time (0.5h,1h,1.5h) and UV light source (6w,11w,15w) were selected as major operating variables. To investigate the effects of variables to degradation rate, the statistical experiment of Box-Behnken design(BBD) and Response Surface methodology(RSM) was employed. Regression analysis showed that the experiment data accorded with the predicted values obtained from quadratic regression equation in BBD with R-Squared of 0.9806 and F-value of 39.34.

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