Thermophilic bacteria that tolerate a wide temperature and pH range colonize the Soldhar (95 °C) and Ringigad (80 °C) hot springs of Uttarakhand, India

2014 ◽  
Vol 65 (2) ◽  
pp. 809-816 ◽  
Author(s):  
Anita Pandey ◽  
Kusum Dhakar ◽  
Avinash Sharma ◽  
Payal Priti ◽  
Priyanka Sati ◽  
...  
Microbiology ◽  
2020 ◽  
Vol 166 (9) ◽  
pp. 800-816
Author(s):  
Ishfaq Nabi Najar ◽  
Nagendra Thakur

The genus Geobacillus , belonging to the phylum Firmicutes, is one of the most important genera and comprises thermophilic bacteria. The genus Geobacillus was erected with the taxonomic reclassification of various Bacillus species. Taxonomic studies of Geobacillus remain in progress. However, there is no comprehensive review of the characteristic features, taxonomic status and study of various applications of this interesting genus. The main aim of this review is to give a comprehensive account of the genus Geobacillus . At present the genus acomprises 25 taxa, 14 validly published (with correct name), nine validly published (with synonyms) and two not validly published species. We describe only validly published species of the genera Geobacillus and Parageobacillus . Vegetative cells of Geobacillus species are Gram-strain-positive or -variable, rod-shaped, motile, endospore-forming, aerobic or facultatively anaerobic, obligately thermophilic and chemo-organotrophic. Growth occurs in the pH range 6.08.5 and a temperature of 37–75 °C. The major cellular fatty acids are iso-C15:o, iso-C16:0 and iso-C17:o. The main menaquinone type is MK-7. The G­+C content of the DNA ranges between 48.2 and 58 mol%. The genus Geobacillus is widely distributed in nature, being mostly found in many extreme locations such as hot springs, hydrothermal vents, marine trenches, hay composts, etc. Geobacillus species have been widely exploited in various industrial and biotechnological applications, and thus are promising candidates for further studies in the future.


2017 ◽  
Vol 8 ◽  
pp. 19-28
Author(s):  
ASHISH DHYANI ◽  
RITU GURURANI ◽  
SAMY A. SELIM ◽  
PRIYANKA ADHIKARI ◽  
AVINASH SHARMA ◽  
...  

Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.


2011 ◽  
Vol 22 ◽  
pp. S88
Author(s):  
Kadriye İnan ◽  
Müslüm Tokgöz ◽  
Sabriye Çanakçi ◽  
Ali Osman Beldüz

2018 ◽  
Vol 44 (1) ◽  
pp. 75-85
Author(s):  
S. A. Alrumman ◽  
Y. S. Mostafa ◽  
Shekha T. S. Al-Qahtani ◽  
T. Sahlabji ◽  
T. H. Taha

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Kenny Lischer ◽  
ANANDA BAGUS RICHKY DIGDAYA PUTRA ◽  
Brian Wirawan Guslianto ◽  
Forbes Avila ◽  
Sarah Grace Sitorus ◽  
...  

Abstract. Lischer K, Putra ABRD, Guslianto BW, Avilla F, Sitorus SG, Nugraha Y, Sarmoko. 2020. Short Communication: The emergence and rise of indigenous thermophilic bacteria exploration from hot springs in Indonesia. Biodiversitas 21: 5474-5481. Indonesia is an archipelagic country located in the pacific ring of fire, and is estimated to cause numerous hot springs spread across the country. In addition, small living microbes have been explored in these locations since 1985. These microbes possess the ability to survive in areas with high temperature (more than 40oC-90oC), and are therefore termed thermophiles. Hence, massive explorations have been conducted on Java island and other unexplored areas at Sumatra to Papua in New Guinea islands. Moreover, a total of 71 hot springs characterized by the presence of thermophilic bacteria have been explored in Indonesia. These investigations ensue with various approaches, including through conventional and microbiological, 16S rRNA, as well as whole-genome sequencing methods. In addition to species exploration, the application of thermophiles has become a topic of interest from 1999, especially based on thermostable enzymes with the capacity to maintain activity at high-temperature conditions. These include amylase, protease, lipase, xylanase, esterase, and cellulase as the most common isolated form, which indicates the existence of significant extractable potentials. Hence, there is a need for further research in terms of both exploration and application purposes.


2009 ◽  
Vol 79 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Ahmet Adiguzel ◽  
Hakan Ozkan ◽  
Ozlem Baris ◽  
Kadriye Inan ◽  
Medine Gulluce ◽  
...  

1972 ◽  
Vol 36 (13) ◽  
pp. 2357-2366 ◽  
Author(s):  
Takashi SAIKI ◽  
Ryohei KIMURA ◽  
Kei ARIMA

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