Analysis of genetic diversity and relationships among waxy maize inbred lines in Korea using SSR markers

The RAPD and SSR markers were used to compare the genetic diversity among the 16 maize inbred lines. Twenty-two primers were used in the RAPD reactions, resulting in the amplification of 265 fragments, while 16 pairs of SSR primers resulted in 75 fragments. The similarity based on Dice coefficient for the RAPD ranged from 53 to 84% and for the SSR from 11 to 82%. The dendrogram obtained by the RAPD showed five groups, while dendrogram obtained by the SSR showed three groups and one isolated line. The association constructed from the markers and the principal coordinate’s analysis separated lines into two groups according to endosperm color, either orange or yellow. The RAPD were effective to validate pedigree data, while the SSR were effective to recognize the differences between the quantitative characters. Because they assess the distinct regions of the genome, the selection of one or other marker would depend on the characteristics of the material used and the objectives of the project.

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Genetic Diversity among CIMMYT Maize Inbred Lines Investigated with SSR Markers

Crop Science ◽

10.2135/cropsci2004.2230 ◽

2004 ◽

Vol 44 (6) ◽

pp. 2230-2237 ◽

Cited By ~ 51

Author(s):

X. C. Xia ◽

J. C. Reif ◽

D. A. Hoisington ◽

A. E. Melchinger ◽

M. Frisch ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Inbred Lines ◽

Maize Inbred Lines

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Genetic diversity among tropical maize inbred lines as revealed by SSR markers

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.12.1589.pdf ◽

2020 ◽

pp. 2010-2019

Author(s):

Maizura Abu Sin ◽

Ghizan Saleh ◽

Nur Ashikin Psyquay Abdullah ◽

Pedram Kashiani

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Gene Diversity ◽

Polymorphic Information Content ◽

Similarity Index ◽

Inbred Lines ◽

Hybrid Breeding ◽

Heterotic Groups ◽

Breeding Programs ◽

Maize Inbred Lines

Genetic diversity and phenotypic superiority are important attributes of parental inbred lines for use in hybrid breeding programs. In this study, genetic diversity among 30 maize (Zea mays L.) inbred lines comprising of 28 introductions from the International Maize and Wheat Improvement Center (CIMMYT), one from Indonesia and a locally developed, were evaluated using 100 simple sequence repeat (SSR) markers, as early screening for potential parents of hybrid varieties. All markers were polymorphic, with a total of 550 unique alleles detected on the 100 loci from the 30 inbred lines. Allelic richness ranged from 2 to 13 per locus, with an average of 5.50 alleles (na). Number of effective alleles (ne) was 3.75 per locus, indicating their high effectiveness in revealing diversity among inbred lines. Average polymorphic information content (PIC) was 0.624, with values ranging from 0.178 to 0.874, indicating high informativeness of the markers. High gene diversity was observed on Chromosomes 8 and 4, with high number of effective alleles, indicating their potential usefulness for QTL analysis. The UPGMA dendrogram constructed identified four heterotic groups within a similarity index of 0.350, indicating that these markers were able to group the inbred lines. The three-dimensional PCoA plot also supports the dendrogram grouping, indicating that these two methods complement each other. Inbred lines in different heterotic groups have originated from different backgrounds and population sources. Information on genetic diversity among the maize inbred lines are useful in developing strategies exploiting heterosis in breeding programs

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Analysis of Population Structure and Genetic Diversity among Canadian Maize Inbred Lines using SSR Markers

Korean Journal of Breeding Science ◽

10.9787/kjbs.2017.49.2.72 ◽

2017 ◽

Vol 49 (2) ◽

pp. 072-79

Author(s):

Tak Ki Hong ◽

Kyu Jin Sa ◽

Ju Kyong Lee

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Ssr Markers ◽

Inbred Lines ◽

Maize Inbred Lines

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Changes of genetic diversity of maize inbred lines over four decades of hybrid breeding in the Bc institute revealed by SSR markers

Genetika ◽

10.2298/gensr1501233b ◽

2015 ◽

Vol 47 (1) ◽

pp. 233-243

Author(s):

Ivica Buhinicek ◽

Mirko Jukic ◽

Hrvoje Sarcevic ◽

Jerko Gunjaca ◽

Zdravko Kozic ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Inbred Lines ◽

Hybrid Breeding ◽

Maize Breeding ◽

Hybrid Production ◽

The Past ◽

Maize Inbred Lines ◽

Heterotic Pattern ◽

Private Alleles

In this paper, changes of genetic diversity of the most important maize inbred lines used for hybrid production within the Bc Institute in the 1970s, 1980s, 1990s and 2000s were examined using the SSR markers. The average number of alleles per SSR locus was 3.14, 3.43, 3.07 and 3.25 for lines from 1970s, 1980s, 1990s and 2000s, whereas the number of private alleles for the same four decades was 8, 4, 0 and 6, respectively. Mean genetic distance among inbreds within decades steadily decreased over time from 0.64 in 1970s to 0.57 in 2000s, but the observed differences were not statistically significant. The clustering of the studied inbred lines indicates the exploitation of a known BSSS x LSC heterotic pattern within the Bc Institute maize breeding program. The overall results show that recycled inbred lines within these pools do not decline in genetic variation over the past 40 years.

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Genetic diversity of maize inbred lines as inferred from SSR markers

Genetika ◽

10.2298/gensr1502489n ◽

2015 ◽

Vol 47 (2) ◽

pp. 489-498

Author(s):

Ana Nikolic ◽

Dragana Ignjatovic-Micic ◽

Dragan Kovacevic ◽

Zoran Camdzija ◽

Milomir Filipovic ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Principal Component ◽

Inbred Lines ◽

Yield Potential ◽

Pedigree Data ◽

Maize Breeding ◽

Breeding Programs ◽

Maize Inbred Lines ◽

The World

Creating new maize hybrids with greater yield potential is a permanent goal of breeding programs all over the world. Long-time existing and new problems related to different biotic and abiotic stresses and the growing needs of the world market require constant work on finding new ways for advancing maize production. Molecular marker technology is one of the fastest developing fields and its implementation has already given results in solving different problems related to maize breeding improvement. The aim of the study presented herein was characterization and genetic similarity assessment of twenty-nine maize inbred lines from Maize Research Institute collection using Simple Sequence Repeats (SSR) markers. The analysis was done using 20 pairs of SSR primers with clearly visible and reproducible results. A total of 119 alleles were detected with a mean of 5.8 per locus. PIC (Polymorphism Information Content) values were in the range from 0.45 to 0.92 (average 0.74). Genetic similarities calculated using Jaccard?s coefficient ranged from 0.27 to 0.99. Cluster and Principal Component Analysis (PCA) analysis were done using matrices of similarity in the NTSYSpc software, version 2.1. Results of both classifications were moderately in agreement with the pedigree data of analysed genotypes. The information about genetic diversity of maize inbred lines revealed by SSR markers could be useful in planning strategies for future maize breeding programs.

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SSR-based and carotenoid diversity assessment of tropical yellow endosperm maize inbred lines

Plant Genetic Resources ◽

10.1017/s1479262113000294 ◽

2013 ◽

Vol 12 (1) ◽

pp. 67-73 ◽

Cited By ~ 2

Author(s):

Oyenike Adeyemo ◽

Olusesan Omidiji

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Polymorphic Information Content ◽

Vitamin A Deficiency ◽

Inbred Lines ◽

Reproductive Age ◽

Sub Saharan Africa ◽

Provitamin A ◽

Maize Inbred Lines ◽

Diversity Assessment

Yellow endosperm maize can be used to reduce vitamin A deficiency among many pre-school children and women of reproductive age in sub-Saharan Africa. Assessment of the genetic diversity of tropical yellow endosperm maize inbred lines will have genetic gains in breeding design to develop lines with an enhanced level of provitamin A. We screened 122 tropical yellow endosperm maize inbred lines with 62 simple sequence repeat (SSR) markers and 51 SSR loci were polymorphic. We detected 190 alleles with an average of 3.72 alleles per locus, and polymorphic information content values among the inbred lines varied from 0.12 to 0.74 with an average of 0.43. Genetic distance (GD) values among all the pairs of the 122 inbred lines varied from 0.02 to 0.61 with an average of 0.41 for the SSR markers. The inbred lines exhibited a substantial level of genetic diversity. Genetic diversity was also evaluated using carotenoid data. The Euclidean GDs varied from 1.00 to 9.97 with an average of 3.81 for the carotenoid data. Cluster and principal coordinate analyses revealed clear separation of maize inbred lines into SSR-based groupings and carotenoid-based groupings. Cluster based on SSR markers were predominantly consistent with known pedigree data of the inbred lines. The correlation using Mantel's test between carotenoid-based GD estimates and SSR marker-based GD estimates gave a low r value ( − 0.06). The grouping of lines will facilitate the selection of parental lines for making crosses to develop new lines with enhanced provitamin A content.

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