scholarly journals Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

2013 ◽  
Vol 24 (3) ◽  
pp. 342-348 ◽  
Author(s):  
Muhammad Munir ◽  
Siamak Zohari ◽  
Muhammad Abbas ◽  
Muhammad Zubair Shabbir ◽  
Muhammad Nauman Zahid ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of H4N6 Avian Influenza Viruses from Pigs with Pneumonia in Canada

2000 ◽  
Vol 74 (19) ◽  
pp. 9322-9327 ◽  
Author(s):  
Alexander I. Karasin ◽  
Ian H. Brown ◽  
Suzanne Carman ◽  
Christopher W. Olsen
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Phylogenetic Analyses ◽  
Influenza Viruses ◽  
Interspecies Transmission ◽  
Influenza A Viruses ◽  
Avian Influenza Viruses ◽  
The North ◽  
Isolation And Characterization

ABSTRACT In October 1999, H4N6 influenza A viruses were isolated from pigs with pneumonia on a commercial swine farm in Canada. Phylogenetic analyses of the sequences of all eight viral RNA segments demonstrated that these are wholly avian influenza viruses of the North American lineage. To our knowledge, this is the first report of interspecies transmission of an avian H4 influenza virus to domestic pigs under natural conditions.

scholarly journals Characterization of H9N2 influenza A viruses isolated from chicken products imported into Japan from China

2006 ◽  
Vol 135 (3) ◽  
pp. 386-391 ◽  
Author(s):  
M. MASE ◽  
M. ETO ◽  
K. IMAI ◽  
K. TSUKAMOTO ◽  
S. YAMAGUCHI
Keyword(s):  
Influenza Virus ◽  
Amino Acid ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Influenza A ◽  
H9n2 Virus ◽  
Amino Acid Substitutions ◽  
Influenza A Viruses ◽  
Potential Sources

We characterized eleven H9N2 influenza A viruses isolated from chicken products imported from China. Genetically they were classified into six distinct genotypes, including five already known genotypes and one novel genotype. This suggested that such multiple genotypes of the H9N2 virus have possibly already become widespread and endemic in China. Two isolates have amino-acid substitutions that confer resistance to amantadine in the M2 region, and this supported the evidence that this mutation might be a result of the wide application of amantadine for avian influenza treatment in China. These findings emphasize the importance of surveillance for avian influenza virus in this region, and of quarantining imported chicken products as potential sources for the introduction of influenza virus.

scholarly journals Antigenic Characterization of H3 Subtypes of Avian Influenza A Viruses from North America

2016 ◽  
Vol 60 (1s) ◽  
pp. 346 ◽  
Author(s):  
Elizabeth Bailey ◽  
Li-Ping Long ◽  
Nan Zhao ◽  
Jeffrey S. Hall ◽  
John A. Baroch ◽  
...  
Keyword(s):  
North America ◽  
Avian Influenza ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Antigenic Characterization

Isolation and characterization of H7N9 avian influenza A virus from humans with respiratory diseases in Zhejiang, China

2014 ◽  
Vol 189 ◽  
pp. 158-164 ◽  
Author(s):  
Yanjun Zhang ◽  
Haiyan Mao ◽  
Juying Yan ◽  
Lei Zhang ◽  
Yi Sun ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A Virus ◽  
Influenza A ◽  
Respiratory Diseases ◽  
Isolation And Characterization ◽  
Avian Influenza A Virus

Genetic and biological characterization of two reassortant H5N2 avian influenza A viruses isolated from waterfowl in China in 2016

2018 ◽  
Vol 224 ◽  
pp. 8-16 ◽  
Author(s):  
Kaituo Liu ◽  
Ruyi Gao ◽  
Min Gu ◽  
Juan Li ◽  
Liwei Shi ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Biological Characterization ◽  
Influenza A Viruses

Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan

2015 ◽  
Vol 160 (12) ◽  
pp. 3037-3052 ◽  
Author(s):  
Kosuke Okuya ◽  
Toshiko Kawabata ◽  
Kiori Nagano ◽  
Kyoko Tsukiyama-Kohara ◽  
Isamu Kusumoto ◽  
...  
Keyword(s):  
Influenza A ◽  
Migratory Birds ◽  
Environmental Water ◽  
Influenza A Viruses ◽  
Isolation And Characterization ◽  
Overwintering Site

scholarly journals Isolation and Genetic Characterization of H5N2 Influenza Viruses from Pigs in Korea

2009 ◽  
Vol 83 (9) ◽  
pp. 4205-4215 ◽  
Author(s):  
Jun Han Lee ◽  
Philippe Noriel Q. Pascua ◽  
Min-Suk Song ◽  
Yun Hee Baek ◽  
Chul-Joong Kim ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Animal Experiments ◽  
Influenza Viruses ◽  
Surface Proteins ◽  
Intermediate Hosts ◽  
Influenza A Viruses ◽  
Highly Pathogenic ◽  
Eurasian Lineage

ABSTRACT Due to dual susceptibility to both human and avian influenza A viruses, pigs are believed to be effective intermediate hosts for the spread and production of new viruses with pandemic potential. In early 2008, two swine H5N2 viruses were isolated from our routine swine surveillance in Korea. The sequencing and phylogenetic analysis of surface proteins revealed that the Sw/Korea/C12/08 and Sw/Korea/C13/08 viruses were derived from avian influenza viruses of the Eurasian lineage. However, although the Sw/Korea/C12/08 isolate is an entirely avian-like virus, the Sw/Korea/C13/08 isolate is an avian-swine-like reassortant with the PB2, PA, NP, and M genes coming from a 2006 Korean swine H3N1-like virus. The molecular characterization of the two viruses indicated an absence of significant mutations that could be associated with virulence or binding affinity. However, animal experiments showed that the reassortant Sw/Korea/C13/08 virus was more adapted and was more readily transmitted than the purely avian-like virus in a swine experimental model but not in ferrets. Furthermore, seroprevalence in swine sera from 2006 to 2008 suggested that avian H5 viruses have been infecting swine since 2006. Although there are no known potential clinical implications of the avian-swine reassortant virus for pathogenicity in pigs or other species, including humans, at present, the efficient transmissibility of the swine-adapted H5N2 virus could facilitate virus spread and could be a potential model for pandemic, highly pathogenic avian influenza (e.g., H5N1 and H7N7) virus outbreaks or a pandemic strain itself.

scholarly journals Molecular Characterization of Highly Pathogenic H5N1 Avian Influenza A Viruses Isolated from Raccoon Dogs in China

PLoS ONE ◽  
2009 ◽  
Vol 4 (3) ◽  
pp. e4682 ◽  
Author(s):  
Xian Qi ◽  
Xihan Li ◽  
Paul Rider ◽  
Weixing Fan ◽  
Hongwei Gu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Molecular Characterization ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza ◽  
Pathogenic H5n1 ◽  
Raccoon Dogs

scholarly journals An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Emily S. Bailey ◽  
Xinye Wang ◽  
Mai-juan Ma ◽  
Guo-lin Wang ◽  
Gregory C. Gray
Keyword(s):  
Influenza A ◽  
Influenza Viruses ◽  
Time Range ◽  
Influenza B ◽  
Influenza A Viruses ◽  
Laboratory Methods ◽  
Duke University ◽  
Multiple Viral Strains ◽  
Rapid Characterization

AbstractInfluenza viruses are an important cause of disease in both humans and animals, and their detection and characterization can take weeks. In this study, we sought to compare classical virology techniques with a new rapid microarray method for the detection and characterization of a very diverse, panel of animal, environmental, and human clinical or field specimens that were molecularly positive for influenza A alone (n = 111), influenza B alone (n = 3), both viruses (n = 13), or influenza negative (n = 2) viruses. All influenza virus positive samples in this study were first subtyped by traditional laboratory methods, and later evaluated using the FluChip-8G Insight Assay (InDevR Inc. Boulder, CO) in laboratories at Duke University (USA) or at Duke Kunshan University (China). The FluChip-8G Insight multiplexed assay agreed with classical virologic techniques 59 (54.1%) of 109 influenza A-positive, 3 (100%) of the 3 influenza B-positive, 0 (0%) of 10 both influenza A- and B-positive samples, 75% of 24 environmental samples including those positive for H1, H3, H7, H9, N1, and N9 strains, and 80% of 22 avian influenza samples. It had difficulty with avian N6 types and swine H3 and N2 influenza specimens. The FluChip-8G Insight assay performed well with most human, environmental, and animal samples, but had some difficulty with samples containing multiple viral strains and with specific animal influenza strains. As classical virology methods are often iterative and can take weeks, the FluChip-8G Insight Assay rapid results (time range 8 to 12 h) offers considerable time savings. As the FluChip-8G analysis algorithm is expected to improve over time with addition of new subtypes and sample matrices, the FluChip-8G Insight Assay has considerable promise for rapid characterization of novel influenza viruses affecting humans or animals.

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