Isolation and characterization of influenza A viruses from environmental water at an overwintering site of migratory birds in Japan

2015 ◽  
Vol 160 (12) ◽  
pp. 3037-3052 ◽  
Author(s):  
Kosuke Okuya ◽  
Toshiko Kawabata ◽  
Kiori Nagano ◽  
Kyoko Tsukiyama-Kohara ◽  
Isamu Kusumoto ◽  
...  
Keyword(s):  
Influenza A ◽  
Migratory Birds ◽  
Environmental Water ◽  
Influenza A Viruses ◽  
Isolation And Characterization ◽  
Overwintering Site

scholarly journals Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

2013 ◽  
Vol 24 (3) ◽  
pp. 342-348 ◽  
Author(s):  
Muhammad Munir ◽  
Siamak Zohari ◽  
Muhammad Abbas ◽  
Muhammad Zubair Shabbir ◽  
Muhammad Nauman Zahid ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of H4N6 Avian Influenza Viruses from Pigs with Pneumonia in Canada

2000 ◽  
Vol 74 (19) ◽  
pp. 9322-9327 ◽  
Author(s):  
Alexander I. Karasin ◽  
Ian H. Brown ◽  
Suzanne Carman ◽  
Christopher W. Olsen
Keyword(s):  
Avian Influenza ◽  
Influenza A ◽  
Phylogenetic Analyses ◽  
Influenza Viruses ◽  
Interspecies Transmission ◽  
Influenza A Viruses ◽  
Avian Influenza Viruses ◽  
The North ◽  
Isolation And Characterization

ABSTRACT In October 1999, H4N6 influenza A viruses were isolated from pigs with pneumonia on a commercial swine farm in Canada. Phylogenetic analyses of the sequences of all eight viral RNA segments demonstrated that these are wholly avian influenza viruses of the North American lineage. To our knowledge, this is the first report of interspecies transmission of an avian H4 influenza virus to domestic pigs under natural conditions.

scholarly journals An evaluation of the InDevR FluChip-8G insight microarray assay in characterizing influenza a viruses

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Emily S. Bailey ◽  
Xinye Wang ◽  
Mai-juan Ma ◽  
Guo-lin Wang ◽  
Gregory C. Gray
Keyword(s):  
Influenza A ◽  
Influenza Viruses ◽  
Time Range ◽  
Influenza B ◽  
Influenza A Viruses ◽  
Laboratory Methods ◽  
Duke University ◽  
Multiple Viral Strains ◽  
Rapid Characterization

AbstractInfluenza viruses are an important cause of disease in both humans and animals, and their detection and characterization can take weeks. In this study, we sought to compare classical virology techniques with a new rapid microarray method for the detection and characterization of a very diverse, panel of animal, environmental, and human clinical or field specimens that were molecularly positive for influenza A alone (n = 111), influenza B alone (n = 3), both viruses (n = 13), or influenza negative (n = 2) viruses. All influenza virus positive samples in this study were first subtyped by traditional laboratory methods, and later evaluated using the FluChip-8G Insight Assay (InDevR Inc. Boulder, CO) in laboratories at Duke University (USA) or at Duke Kunshan University (China). The FluChip-8G Insight multiplexed assay agreed with classical virologic techniques 59 (54.1%) of 109 influenza A-positive, 3 (100%) of the 3 influenza B-positive, 0 (0%) of 10 both influenza A- and B-positive samples, 75% of 24 environmental samples including those positive for H1, H3, H7, H9, N1, and N9 strains, and 80% of 22 avian influenza samples. It had difficulty with avian N6 types and swine H3 and N2 influenza specimens. The FluChip-8G Insight assay performed well with most human, environmental, and animal samples, but had some difficulty with samples containing multiple viral strains and with specific animal influenza strains. As classical virology methods are often iterative and can take weeks, the FluChip-8G Insight Assay rapid results (time range 8 to 12 h) offers considerable time savings. As the FluChip-8G analysis algorithm is expected to improve over time with addition of new subtypes and sample matrices, the FluChip-8G Insight Assay has considerable promise for rapid characterization of novel influenza viruses affecting humans or animals.

scholarly journals Molecular and biological characterization of influenza A viruses isolated from human fecal samples

2021 ◽  
pp. 104972
Author(s):  
Hebah A. Al Khatib ◽  
Peter V. Coyle ◽  
Muna A. Al Maslamani ◽  
Asmaa A. Al Thani ◽  
Sameer A. Pathan ◽  
...  
Keyword(s):  
Influenza A ◽  
Biological Characterization ◽  
Influenza A Viruses ◽  
Fecal Samples

scholarly journals Antigenic characterization of novel H1 influenza A viruses in swine

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rodrigo Tapia ◽  
Montserrat Torremorell ◽  
Marie Culhane ◽  
Rafael A. Medina ◽  
Víctor Neira
Keyword(s):  
Influenza A ◽  
Influenza A Viruses ◽  
Antigenic Characterization

scholarly journals Characterization of H9N2 influenza A viruses isolated from chicken products imported into Japan from China

2006 ◽  
Vol 135 (3) ◽  
pp. 386-391 ◽  
Author(s):  
M. MASE ◽  
M. ETO ◽  
K. IMAI ◽  
K. TSUKAMOTO ◽  
S. YAMAGUCHI
Keyword(s):  
Influenza Virus ◽  
Amino Acid ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Influenza A ◽  
H9n2 Virus ◽  
Amino Acid Substitutions ◽  
Influenza A Viruses ◽  
Potential Sources

We characterized eleven H9N2 influenza A viruses isolated from chicken products imported from China. Genetically they were classified into six distinct genotypes, including five already known genotypes and one novel genotype. This suggested that such multiple genotypes of the H9N2 virus have possibly already become widespread and endemic in China. Two isolates have amino-acid substitutions that confer resistance to amantadine in the M2 region, and this supported the evidence that this mutation might be a result of the wide application of amantadine for avian influenza treatment in China. These findings emphasize the importance of surveillance for avian influenza virus in this region, and of quarantining imported chicken products as potential sources for the introduction of influenza virus.

scholarly journals Molecular Characterization of the Hemagglutinin and Neuraminidase Genes of H5N1 Influenza A Viruses Isolated from Poultry in Vietnam from 2004 to 2005

2006 ◽  
Vol 68 (5) ◽  
pp. 527-531 ◽  
Author(s):  
Yukiko MURAMOTO ◽  
Thi Quynh Mai LE ◽  
Lien Song PHUONG ◽  
Tung NGUYEN ◽  
Thu Ha NGUYEN ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Influenza A ◽  
Influenza A Viruses ◽  
H5n1 Influenza

scholarly journals Antigenic Characterization of H3 Subtypes of Avian Influenza A Viruses from North America

2016 ◽  
Vol 60 (1s) ◽  
pp. 346 ◽  
Author(s):  
Elizabeth Bailey ◽  
Li-Ping Long ◽  
Nan Zhao ◽  
Jeffrey S. Hall ◽  
John A. Baroch ◽  
...  
Keyword(s):  
North America ◽  
Avian Influenza ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Antigenic Characterization

scholarly journals Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gulls

2005 ◽  
Vol 79 (5) ◽  
pp. 2814-2822 ◽  
Author(s):  
Ron A. M. Fouchier ◽  
Vincent Munster ◽  
Anders Wallensten ◽  
Theo M. Bestebroer ◽  
Sander Herfst ◽  
...  
Keyword(s):  
Influenza A ◽  
Rapid Identification ◽  
Influenza Surveillance ◽  
Influenza A Viruses ◽  
Receptor Binding Site ◽  
Full Spectrum ◽  
Putative Receptor ◽  
Detection And Identification ◽  
Definition Of

ABSTRACT In wild aquatic birds and poultry around the world, influenza A viruses carrying 15 antigenic subtypes of hemagglutinin (HA) and 9 antigenic subtypes of neuraminidase (NA) have been described. Here we describe a previously unidentified antigenic subtype of HA (H16), detected in viruses circulating in black-headed gulls in Sweden. In agreement with established criteria for the definition of antigenic subtypes, hemagglutination inhibition assays and immunodiffusion assays failed to detect specific reactivity between H16 and the previously described subtypes H1 to H15. Genetically, H16 HA was found to be distantly related to H13 HA, a subtype also detected exclusively in shorebirds, and the amino acid composition of the putative receptor-binding site of H13 and H16 HAs was found to be distinct from that in HA subtypes circulating in ducks and geese. The H16 viruses contained NA genes that were similar to those of other Eurasian shorebirds but genetically distinct from N3 genes detected in other birds and geographical locations. The European gull viruses were further distinguishable from other influenza A viruses based on their PB2, NP, and NS genes. Gaining information on the full spectrum of avian influenza A viruses and creating reagents for their detection and identification will remain an important task for influenza surveillance, outbreak control, and animal and public health. We propose that sequence analyses of HA and NA genes of influenza A viruses be used for the rapid identification of existing and novel HA and NA subtypes.

Sign in / Sign up

Export Citation Format

Share Document