An injectable, in situ forming type II collagen/hyaluronic acid hydrogel vehicle for chondrocyte delivery in cartilage tissue engineering

2014 ◽  
Vol 4 (2) ◽  
pp. 149-158 ◽  
Author(s):  
Leena-Stiina Kontturi ◽  
Elina Järvinen ◽  
Virpi Muhonen ◽  
Estelle C. Collin ◽  
Abhay S. Pandit ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type Ii ◽  
In Situ Forming ◽  
Hyaluronic Acid Hydrogel

scholarly journals Injectable in situ forming biodegradable chitosan–hyaluronic acid based hydrogels for cartilage tissue engineering

Biomaterials ◽  
2009 ◽  
Vol 30 (13) ◽  
pp. 2499-2506 ◽  
Author(s):  
Huaping Tan ◽  
Constance R. Chu ◽  
Karin A. Payne ◽  
Kacey G. Marra
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Situ Forming

scholarly journals Anisotropic Shape-Memory Alginate Scaffolds Functionalized with Either Type I or Type II Collagen for Cartilage Tissue Engineering

2017 ◽  
Vol 23 (1-2) ◽  
pp. 55-68 ◽  
Author(s):  
Henrique V. Almeida ◽  
Binulal N. Sathy ◽  
Ivan Dudurych ◽  
Conor T. Buckley ◽  
Fergal J. O'Brien ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shape Memory ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type I ◽  
Type Ii

Human Cartilage Tissue Engineering with Pluronic and Cultured Chondrocyte Sheet

2007 ◽  
Vol 342-343 ◽  
pp. 89-92 ◽  
Author(s):  
Jae Ho Jeong ◽  
Y.M. Moon ◽  
S.O. Kim ◽  
S.S. Yun ◽  
Hong In Shin
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
New Method ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Human Cartilage ◽  
Covered Group ◽  
Chondrocyte Sheet

Despite many outstanding research works on cartilage tissue engineering, actual clinical application is not quite successful because of the absorption and progressive distortion of tissue engineered cartilage. We have developed a new method of cartilage tissue engineering comprising chondrocyte mixed Pluronic F-127 and cultured chondrocyte cell sheet which entirely cover the cell-Pluronic complex. We believe the addition of cultured chondrocyte cell sheet enhances the efficacy of chondrogenesis in vivo. Human ear cartilage piece was enzymatically dissociated and chondrocyte suspension was acquired. Chondrocytes were cultured and expanded as the routine manner. Cultured chondrocytes were plated in high-density monolayer and cultured with Chondrogenic media in 5% CO2 incubator. After 3 weeks of culture, chondrocyte cell sheet was formed and complete single sheet of chondrocyte could be harvested by gentle manipulation of culture plate with a cell scraper. Chondrocyte-Pluronic complex was established by mixing 1x 106 cells with 0.5 of Pluronic F- 127. Chondrocyte-Pluronic complex was completely covered with a sheet of cultured chondrocyte. The completed tissue engineered constructs were implanted into the subcutaneous tissue pocket of nude mice on the back. Tissue engineered constructs without cultured cell sheet were used as control. Samples were harvested at 8 weeks postoperatively and they were subjected to histological analysis and assayed for glycosaminoglycan (GAG), and type II collagen. Grossly, the size of cartilage specimen of cultured chondrocyte cell sheet covered group was larger than that of the control. On histologic examination, the specimen of cultured chondrocyte cell sheet covered group showed lacunae-containing cells embedded in a basophilic matrix. The chondrocyte cell sheet covered group specimen resembled mature or immature cartilage. The result of measurement of GAG and type II collagen of cartilage specimen of cultured chondrocyte sheet covered group was higher than that of the control. In conclusion, the new method of cartilage tissue engineering using chondrocyte cell sheet seems to be an effective method providing higher cartilage tissue gain and reliable success rate for cartilage tissue engineering.

Recombinant human type II collagen as a material for cartilage tissue engineering

2008 ◽  
Vol 31 (11) ◽  
pp. 960-969 ◽  
Author(s):  
H.J. Pulkkinen ◽  
V. Tiitu ◽  
P. Valonen ◽  
E.-R. Hämäläinen ◽  
M.J. Lammi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Confocal Microscopy ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Homogeneous Distribution ◽  
Type Ii ◽  
Human Type ◽  
Cultivation Period

Purpose Collagen type II is the major component of cartilage and would be an optimal scaffold material for reconstruction of injured cartilage tissue. In this study, the feasibility of recombinant human type II collagen gel as a 3-dimensional culture system for bovine chondrocytes was evaluated in vitro. Methods Bovine chondrocytes (4x106 cells) were seeded within collagen gels and cultivated for up to 4 weeks. The gels were investigated with confocal microscopy, histology, and biochemical assays. Results Confocal microscopy revealed that the cells maintained their viability during the entire cultivation period. The chondrocytes were evenly distributed inside the gels, and the number of cells and the amount of the extracellular matrix increased during cultivation. The chondrocytes maintained their round phenotype during the 4-week cultivation period. The glycosaminoglycan levels of the tissue increased during the experiment. The relative levels of aggrecan and type II collagen mRNA measured with realtime polymerase chain reaction (PCR) showed an increase at 1 week. Conclusion Our results imply that recombinant human type II collagen is a promising biomaterial for cartilage tissue engineering, allowing homogeneous distribution in the gel and biosynthesis of extracellular matrix components.

Injectable in situ-forming hydrogel for cartilage tissue engineering

2013 ◽  
Vol 1 (26) ◽  
pp. 3314 ◽  
Author(s):  
Jin Seon Kwon ◽  
So Mi Yoon ◽  
Doo Yeon Kwon ◽  
Da Yeon Kim ◽  
Guo Zhe Tai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Situ Forming

In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering

2018 ◽  
Vol 9 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Sang-Soo Han ◽  
Hong Yeol Yoon ◽  
Ji Young Yhee ◽  
Myeong Ok Cho ◽  
Hye-Eun Shim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Click Chemistry ◽  
Click Reaction ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue

We develop a biocompatible and in situ HA hydrogel via a bioorthogonal click reaction for cartilage tissue engineering.

Fabrication of modified dextran–gelatin in situ forming hydrogel and application in cartilage tissue engineering

2014 ◽  
Vol 2 (47) ◽  
pp. 8346-8360 ◽  
Author(s):  
Jian-feng Pan ◽  
Liu Yuan ◽  
Chang-an Guo ◽  
Xiao-hua Geng ◽  
Teng Fei ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
In Situ Forming

scholarly journals An injectable, click-crosslinked, cytomodulin-modified hyaluronic acid hydrogel for cartilage tissue engineering

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Seung Hun Park ◽  
Ji Young Seo ◽  
Joon Yeong Park ◽  
Yun Bae Ji ◽  
Kyungsook Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hyaluronic Acid Hydrogel
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