scholarly journals The Agrobacterium-mediated transformation of common wheat (Triticum aestivum L.) and triticale (x Triticosecale Wittmack): role of the binary vector system and selection cassettes

2011 ◽  
Vol 53 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Agnieszka Bińka ◽  
Wacław Orczyk ◽  
Anna Nadolska-Orczyk
1989 ◽  
Vol 69 (1) ◽  
pp. 1-7 ◽  
Author(s):  
J. M. CLARKE ◽  
R. M. DePAUW

The rate of water imbibition by wheat kernels may be related to preharvest sprouting damage and tempering times during milling. The effects of kernel color and exposure to weather damage on water imbibition rate of wheat (Triticum aestivum L.) kernels, and the effects of field vs. oven drying and hand vs. mechanial threshing on water uptake rate of HY320 wheat and Welsh triticale (X-Triticosecale Wittmack) were investigated. Rates of imbibition were determined by sequential weighings over a 32-h period of 50-kernel samples imbibing water from agar media. In HY320 wheat, the rate was faster for mechanically threshed (0.0117 g g−1 h−1) than for hand-threshed (0.0115 g g−1 h−1) samples. Threshing method did not affect imbibition rate of Welsh triticale kernels (average 0.0141 g g−1 h−1). Rate of germination was significantly greater for mechanically threshed than for hand-threshed Welsh, but there was no significant difference for HY320. Method of drying did not affect kernel water imbibition rate. Rate of imbibition was faster in nonweathered than in weathered wheat (0.0136 vs. 0.0130 g g−1 h−1). In five wheat crosses involving white and red kernel color, rate of water imbibition was not associated with the allele for kernel color. Rate was negatively correlated with kernel weight (r = 0.49**, n = 49) and kernel hardness (r = −0.29*) in the five crosses, and positively correlated with protein content (r = 0.44**). Other undetermined factors accounted for the major part of the genotypic differences in rate of imbibition.Key words: Triticum aestivum L., X-Triticosecale Wittmack, windrowing, kernel water imbibition rate, germination rate


1988 ◽  
Vol 68 (2) ◽  
pp. 361-366 ◽  
Author(s):  
B. L. McINTYRE ◽  
T. H. H. CHEN ◽  
M. F. MEDERICK

Several traits have been measured as indices for winter hardiness in winter wheat (Triticum aestivum L. em. Thell). Published information on the response of winter triticale (X Triticosecale Wittmack) to these traits is limited. In this study LT50, water content, fresh weight, dry weight and total soluble carbohydrate (TSC) were determined for cold acclimated crowns of 10 breeding lines of T. aestivum and 18 of triticale. The T. aestivum lines evaluated were hardier than the triticale and LT50 appeared to be a reliable predictor of field survival (FSI). Correlations between LT50 and FSI were significant for both species. Correlation coefficients between traits measured differed between species. In triticale, correlations between dry weight, water content and LT50 were significant. In T. aestivum water content correlated closest with LT50. The relationship between TSC and FSI appeared to differ between the two species.Key words: X Triticosecale Wittmack, Triticum aestivum L. em. Thell, wheat (winter), cold hardiness, primary triticale, secondary triticale


2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.


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