Optimization of peroxide-alkaline pretreatment and enzymatic hydrolysis of barley straw (Hordeum vulgare L.) to produce fermentable sugars using a Box–Behnken design

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N. Ibarra-Díaz ◽  
J. F. Castañón-Rodríguez ◽  
J. Gómez-Rodríguez ◽  
M. G. Aguilar-Uscanga
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Juan Carlos Farías-Sánchez ◽  
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Agustín Jaime Castro-Montoya

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One of the crucial steps in the enzymatic hydrolysis of biomass is pretreatment. We investigated the applicability of a hybrid pretreatment method, This involved acid treatment followed by alkali before the production of fermentable sugars from cassava stem. The substrate was initially treated with acid (1% oxalic acid at 100 °C for 2 h), followed by treatment with a 10% NaOH–urea mixture (by mixing 5 g each of NaOH and urea in 100 mL of water) at 100 °C for 1 h. A cellulose recovery of 98.55% was obtained with the acid-alkali pretreatment method.


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