Statistical optimization of dilute acid and H2O2 alkaline pretreatment using surface response methodology and tween 80 for the enhancement of the enzymatic hydrolysis of corncob

Abstract Background: During dilute acid pretreatment, pseudo lignin and lignin form droplets which deposit on the surface of lignocellulose, and further inhibit its enzymatic hydrolysis. However, how this lignin interacts with cellulase enzymes and then affects enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by milled wood lignin (MWL) method. All the obtained lignin fractions from DAP-BR were used to investigate the interaction mechanism between lignin and cellulase using surface plasmon resonance (SPR) technology in order to understand how they affect enzymatic hydrolysisResults: Results showed that removing surface lignin significantly decrease the enzymatic hydrolysis of DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel decreased enzymatic hydrolysis of Avicel, while different SL samples showed a slight increase to its enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus the SL samples, stronger affinity for MWL (KD = 6.8-24.7 nM) was found versus that of SL (KD = 39.4-52.6 nM) by SPR analysis. The affinity constant of all tested lignin had good correlations (R2>0.6) with their effects on enzymatic digestibility of extracted DAP-BR and Avicel.Conclusions: This work reveals that the surface lignin on DAP-BR is necessary towards maintaining enzyme digestibility levels, and its removal has a negative impact on the substrate’s digestibility.

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Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

Journal of Energy ◽

10.1155/2016/8506214 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 20

Author(s):

Robinson Timung ◽

Narendra Naik Deshavath ◽

Vaibhav V. Goud ◽

Venkata V. Dasu

Keyword(s):

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Crystallinity Index ◽

Sem Analysis ◽

Reducing Sugar ◽

Dilute Acid Pretreatment ◽

Crystalline Cellulose ◽

Dilute Acid ◽

Acid Pretreatment ◽

Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

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Enhancing enzymatic hydrolysis of green coconut fiber—Pretreatment assisted by tween 80 and water effect on the post-washing

Industrial Crops and Products ◽

10.1016/j.indcrop.2017.12.047 ◽

2018 ◽

Vol 112 ◽

pp. 734-740 ◽

Cited By ~ 18

Author(s):

Cleitiane da Costa Nogueira ◽

Carlos Eduardo de Araújo Padilha ◽

Ana Laura de Sá Leitão ◽

Patrícia Maria Rocha ◽

Gorete Ribeiro de Macedo ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Tween 80 ◽

Water Effect ◽

Coconut Fiber ◽

Hydrolysis Of

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Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production

Renewable Energy ◽

10.1016/j.renene.2020.01.101 ◽

2020 ◽

Vol 152 ◽

pp. 627-633 ◽

Cited By ~ 3

Author(s):

Katarina Mihajlovski ◽

Mirjana Rajilić-Stojanović ◽

Suzana Dimitrijević-Branković

Keyword(s):

Enzymatic Hydrolysis ◽

Statistical Optimization ◽

Bioethanol Production ◽

Hydrolysis Of

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Effects of Rhamnolipids on Enzymatic Hydrolysis of Bamboo Biomass and Mechanism

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2020.1985 ◽

2020 ◽

Vol 14 (4) ◽

pp. 453-460

Author(s):

Ruyi Sha ◽

Zhan Yu ◽

Zhenzhen Wang ◽

Edwin Menledy Gbor ◽

Ligang Jiang ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Fluorescence Probe ◽

Economic Losses ◽

Dilute Acid ◽

Site Of Action ◽

The Stability ◽

Surface Modified ◽

Hydrolysis Of ◽

Positive Effect

The lignin present in lignocellulose seriously affects the efficiency of cellulose enzymatic hydrolysis. In addition, lignin adsorbs high-cost cellulase, causing greater economic losses. Lignin can also disturb the site of action of cellulase and reduce the efficiency of hydrolysis. Therefore, if lignin is removed or surface modified before cellulose enzymatic hydrolysis, the enzymatic hydrolysis efficiency of lignocellulosic biomass will be greatly improved. In this paper, the cellulose enzymatic properties of bamboo biomass being treated with dilute acid and alkaline under the intervention of biosurfactant rhamnolipid were evaluated. The effects of rhamnolipids on the adsorption characterization of cellulose on pretreated bamboo were studied. Besides, the inter-communication between rhamnolipids and cellulose was investigated by fluorescence probe. The results showed that rhamnolipids could have a positive effect on the enzymatic hydrolysis of bamboo biomass by reducing the non-productive adsorption of cellulase on the surface of lignocellulose. The outcome illustrated that cellulase could be combined with rhamnolipids micelles, participating in the formation of rhamnolipids micelles, thereby increasing the internal hydrophobicity of the micelles, but could not change the properties of rhamnolipids micelles higher than one CMC (Critical Micelle Concentration). It can be seen that the interaction between rhamnolipids and cellulase is beneficial to enhance the stability and enzymatic activity of cellulase, thereby improving the enzymatic hydrolysis efficiency of cellulose in biomass. Based on these results, a theoretical knowledge about the mechanism of enhancing the enzymatic hydrolysis efficiency of lignocellulose by biosurfactants rhamnolipids is provided.

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