Evaluation of chlorophyll fluorescence parameters and proline content in tomato seedlings grown under different salt stress conditions

Salinity is one of the major stresses that limits crop production worldwide and affects most physiological activities in plants. In order to study the genetic control of salt stress in the model legume Medicago truncatula Gaertn., an experiment was undertaken to determine the genetic variability and to identify quantitative trait loci (QTLs) controlling several traits related to plant growth and physiology in a population of recombinant inbred lines. Shoot and root DW, relative water content, leaf area, chlorophyll content, chlorophyll fluorescence parameters, and Na+ and K+ in shoots and roots were measured. The experiment was carried out with three replications. ANOVA showed a large genetic variation and transgressive segregation for the traits studied, suggesting putative complex tolerance mechanisms. A total of 21 QTLs were detected under control conditions and 19 QTLs were identified under 100 mm salt stress conditions, with three QTLs being common to both situations. The percentage of total phenotypic variance explained by the QTLs ranged from 4.6% to 23.01%. Overlapping QTLs for different traits were also observed, which enables us to discriminate independent traits from linked ones. The results should be helpful information for further functional analysis of salt tolerance in M. truncatula.

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Effects of sea salt stress on reflectance spectrum and chlorophyll fluorescence parameters inBambusa oldhamiileaves

Acta Ecologica Sinica ◽

10.5846/stxb201309232337 ◽

2014 ◽

Vol 34 (17) ◽

Author(s):

吴寿国 WU Shouguo ◽

余学军 YU Xuejun ◽

李凯 LI Kai ◽

蒋玉俭 JIANG Yujian ◽

张汝民 ZHANG Rumin

Keyword(s):

Salt Stress ◽

Chlorophyll Fluorescence ◽

Reflectance Spectrum ◽

Sea Salt ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters

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Changes in chlorophyll fluorescence parameters during desiccation and osmotic stress of Hassallia antarctica culture

Czech Polar Reports ◽

10.5817/cpr2018-2-16 ◽

2018 ◽

Vol 8 (2) ◽

pp. 198-207

Author(s):

Marta Campos Alonso

Keyword(s):

Salt Stress ◽

Chlorophyll Fluorescence ◽

Osmotic Stress ◽

Austral Summer ◽

Rapid Decline ◽

Filamentous Cyanobacterium ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Fluorescence Measurements ◽

James Ross Island

Hassallia antarctica is a terrestrial cyanobacterium colonizing various habitats in Antarctica such as soil surface, microbiological mats and seepages. H. antarctica represents one of the cyanobacterial species forming biodiversity of terrestrial autotrophs of James Ross Island, Antarctica. It is a filamentous cyanobacterium composing blackish fasciculated clusters thanks to false branching. In our study, sensitivity of the species to dehydration and salt stress was studied. We used H. antarctica culture (CCALA 956) grown on Z liquid medium. Clusters of H. antarctica were placed on wet filter paper and dried naturally at 5°C. During gradual dehydration, relative water content (RWC) was evaluated gravimetrically simultaneously with chlorophyll fluorescence measurements. Slow Kautsky kinetics and the chlorophyll fluorescence parameters (FV/FM, ФPSII) were used to assess dehydration-related decrease in primary photosynthetic processes. It was found that H. antarctica, contrastingly to other terrestrial cyanobacteria from polar habitats, was not able to maintain photosynthetic processes at RWCs as low as 20%. Even during initial phase of dehydration (RWC of 95%) rapid decline in FV/FM occured. Resistance of H. antarctica to osmotic stress was studied by time courses of the chlorophyll fluorescence parameter in response to 3.0, 0.3, and 0.03 M NaCl solution. Both shape of slow Kautsky kinetics and numeric values of chlorophyll fluorescence parameters were affected by osmotic stress. While full inhibitory effect was apparent in 3.0 M NaCl treatment immediately, the salt stress-induced decline in chlorophyll fluorescence parameters was observed at 0.03 M NaCl even after 8 hours of exposition. It was, therefore, concluded that H. antarctica exhibited high resistance to osmotic stress which may help the species to cope with repetitive dehydration events that happen in the field during austral summer season in Antarctica, James Ross Island in particular.

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Glycinebetaine Improved Photosynthesis in Canola under Salt Stress: Evaluation of Chlorophyll Fluorescence Parameters as Potential Indicators

Journal of Agronomy and Crop Science ◽

10.1111/jac.12120 ◽

2015 ◽

Vol 201 (6) ◽

pp. 428-442 ◽

Cited By ~ 30

Author(s):

H.-u.-R. Athar ◽

Z. U. Zafar ◽

M. Ashraf

Keyword(s):

Salt Stress ◽

Chlorophyll Fluorescence ◽

Chlorophyll Fluorescence Parameters ◽

Stress Evaluation ◽

Fluorescence Parameters

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Differential responses of ribulose-1,5-bisphosphate carboxylase/oxygenase activities of two Vigna unguiculata cultivars to salt stress

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202005000200003 ◽

2005 ◽

Vol 17 (2) ◽

pp. 207-212 ◽

Cited By ~ 9

Author(s):

Maria Erivalda Farias de Aragão ◽

Marjorie Moreira Guedes ◽

Maria de Lourdes Oliveira Otoch ◽

Maria Izabel Florindo Guedes ◽

Dirce Fernandes de Melo ◽

...

Keyword(s):

Salt Stress ◽

Chlorophyll Fluorescence ◽

Chlorophyll Content ◽

Vigna Unguiculata ◽

Shoot Growth ◽

Bisphosphate Carboxylase ◽

Chlorophyll Fluorescence Parameters ◽

Fluorescence Parameters ◽

Root And Shoot Growth ◽

Specific Activities

Vita 3 and Vita 5 are two Vigna unguiculata cultivars that differ in their capacities for survival in saline environments; Vita 3 is more tolerant and Vita 5 more sensitive. Both cultivars were submitted to salt stress with 0.1 M NaCl. After 8 days, root and shoot growth from both cultivars was reduced but reduction was more pronounced in Vita 5. Furthermore, leaf area was also reduced in this cultivar. Chlorophyll content and chlorophyll fluorescence parameters were not affected by salt stress, but the specific activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) decreased in Vita 3 and increased in Vita 5. The use of immunological techniques also revealed that the Rubisco content from Vita 3 decreased while that of Vita 5 increased. The discussion of these results is aimed at reaching a better understanding of the differences between these cultivars in relation to salt stress.

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Putrescine-functionalized carbon quantum dot (put-CQD) nanoparticles effectively prime grapevine (Vitis vinifera cv. ‘Sultana’) against salt stress

BMC Plant Biology ◽

10.1186/s12870-021-02901-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Gholamreza Gohari ◽

Sima Panahirad ◽

Mostafa Sadeghi ◽

Ali Akbari ◽

Elnaz Zareei ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Vitis Vinifera ◽

Salinity Stress ◽

Photosynthetic Pigments ◽

Synergistic Effects ◽

Proline Content ◽

Stress Conditions ◽

Plant Performance ◽

Nano Structure ◽

Chlorophyll Fluorescence Parameters

Abstract Background Salinity is an important global problem with destructive impacts on plants leading to different biochemical and metabolic changes in plants through induced oxidative stress that disturbs metabolism, growth, performance and productivity of plants. Given that putrescine (Put) and carbon quantum dots (CQDs), individually, have promising effects in different plant processes, the idea of their combination in a nano-structure “Put-CQD” lead to its synthesis to evaluate the potential exertion of synergistic effects. The current study aimed to investigate the application of newly-synthesized nanoparticles (NPs) consisting of CQDs and Put in grapevine (Vitis vinifera cv. ‘Sultana’) under salinity stress conditions. For this purpose, Put, CQDs and Put-CQD NPs at 5 and 10 mg L− 1 concentrations were applied as chemical priming agents in ‘Sultana’ grapevine 48 h prior salinity stress imposition (0 and 100 mM NaCl). Results Salinity significantly decreased (P ≤ 0.05) morphological parameters, photosynthetic pigments, chlorophyll fluorescence parameters and membrane stability index. In addition, salinity enhanced MDA, H2O2, proline content and antioxidant enzyme activity. Results revealed that Put-CQD NPs, particularly at 10 mg L− 1 concentration, alleviated the destructive impacts of salinity stress by improving leaf fresh and dry weights, K+ content, photosynthetic pigments, chlorophyll fluorescence and SPAD parameters, proline content, total phenolics and antioxidant enzymatic activities (CAT, APX, GP and SOD), while decreasing Na+ content, EL, MDA and H2O2 levels. Conclusion To conclude, Put-CQD NPs represent an innovative priming treatment that could be effectively applied on grapevine to improve plant performance under salinity stress conditions.

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