Lag phase alteration in the modified bovine serum albumin under the inducing and inhibitory effect of vitamin C

2018 ◽  
Vol 15 (6) ◽  
pp. 1337-1346
Author(s):  
E. Vahdat-Ahar ◽  
A. A. Moosavi-Movahedi ◽  
F. Taghavi ◽  
M. Habibi-Rezaei ◽  
N. Sheibani
Keyword(s):  
Bovine Serum Albumin ◽  
Vitamin C ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inhibitory Effect ◽  
Lag Phase

Interaction of aspirin and vitamin C with bovine serum albumin

2011 ◽  
Vol 105 (3) ◽  
pp. 198-202 ◽  
Author(s):  
Shohreh Nafisi ◽  
Golshan Bagheri Sadeghi ◽  
Ataollah PanahYab
Keyword(s):  
Bovine Serum Albumin ◽  
Vitamin C ◽  
Serum Albumin ◽  
Bovine Serum

Studies on the Adsorption and Thermodynamics of Theophylline, Vitamin C and Bovine Serum Albumin on Microporous Corn Starch

2018 ◽  
Vol 71 (3-4) ◽  
pp. 1800042
Author(s):  
Yuhua Li ◽  
Yeli Zhang ◽  
Juan Zhao ◽  
Ning Han ◽  
Liujiao Bian
Keyword(s):  
Bovine Serum Albumin ◽  
Vitamin C ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Corn Starch

Calcium ions reverse a latency period induced by bovine serum albumin in the time course of phospholipase A2 action on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposomes

1993 ◽  
Vol 71 (9-10) ◽  
pp. 462-466 ◽  
Author(s):  
Fermín Pacheco ◽  
Ricardo Mejía ◽  
Marta S. Fernández
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Calcium Ions ◽  
Bovine Serum ◽  
Time Course ◽  
Latency Period ◽  
Phospholipase A ◽  
Ion Concentration ◽  
Lag Phase

The time course of hydrolysis of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine liposomes in the gel phase catalyzed by porcine pancreatic phospholipase A2 was studied at 1 mM NaCl and variable CaCl2 concentrations, in the presence of delipidated bovine serum albumin. It has been found that the duration of the latency induced by bovine serum albumin shows an inverse dependence with CaCl2 concentration. As we showed previously, the induction of a lag phase by bovine serum albumin is related to its ability to sequester the fatty acid newly released by hydrolysis. Based on this and on our observation that there is an inverse dependence between the length of the latency period and the interfacial calcium ion concentration, it is interpreted that, while a direct effect of bovine serum albumin is the diminution of the liposome negative surface charge density by sequestration of the fatty acid released during hydrolysis, an indirect effect could be the decrease in the surface Ca2+ concentration. This, in turn, should diminish the enzyme binding to the lipid–water interface. The appearance of a latency phase seems to be the final consequence of these events.Key words: phospholipase A2, latency period, calcium ions, serum albumin.

Effects of Cereal Prolamin Peptides on Differentiated CaCo-2 Cells

1996 ◽  
Vol 24 (4) ◽  
pp. 547-552
Author(s):  
Claudio Giovannini ◽  
Roberto Luchetti ◽  
Elena Mancini ◽  
Massimo de Vincenzi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cell Viability ◽  
Mtt Assay ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cell Damage ◽  
Inhibitory Effect ◽  
Culture Cell ◽  
Glycoprotein Synthesis

The effects of peptic-tiyptic (PT) digests of prolamins derived from several cereals on differentiated CaCo-2 cells were studied on the nineteenth day of culture. Cell viability was determined by using the MTT assay and the colony-forming ability method. The metabolic consequences of peptide exposure were evaluated by measuring RNA, protein and glycoprotein synthesis. While PT digests from bovine serum albumin and durum wheat did not exert any effects, those derived from bread wheat, barley, rye and oats caused a dramatic inhibitory effect on metabolic synthesis and, when measured by using the colony-forming technique, a decrease in cell viability. The MTT assay did not indicate any changes in cell viability. These observations support the hypothesis that, although prolamin-derived peptides from these cereals do not exert an immediate cytotoxic effect, they are responsible for cell damage by impairment of metabolic processes.

scholarly journals Inhibitory effect of gold nanoparticles on the D-ribose glycation of bovine serum albumin

2014 ◽  
pp. 5461 ◽  
Author(s):  
Weixi Liu ◽  
Menashi Cohenford ◽  
Leslie Frost ◽  
Champika Seneviratne ◽  
Joel Dain
Keyword(s):  
Gold Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inhibitory Effect

Spectroscopic Studies on the Interaction of Vitamin C with Bovine Serum Albumin

2008 ◽  
Vol 38 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Hui Xu ◽  
Quanwen Liu ◽  
Ying Zuo ◽  
Yan Bi ◽  
Shuli Gao
Keyword(s):  
Bovine Serum Albumin ◽  
Vitamin C ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Spectroscopic Studies
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