Inhibitory Effect of Bovine Serum Albumin on Acid Deoxyribonuclease from Rat Small Intestinal Mucosa1

1983 ◽  
Vol 94 (2) ◽  
pp. 345-352 ◽  
Author(s):  
Natsuko ESHIMA ◽  
Atsuko MUTA ◽  
Motoaki ANAI
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inhibitory Effect ◽  
Small Intestinal ◽  
Acid Deoxyribonuclease

Transport of bovine serum albumin across rat jejunum: role of the enteric nervous system

1994 ◽  
Vol 266 (2) ◽  
pp. G186-G193 ◽  
Author(s):  
M. H. Kimm ◽  
G. H. Curtis ◽  
J. A. Hardin ◽  
D. G. Gall
Keyword(s):  
Nervous System ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Enteric Nervous System ◽  
Intestinal Mucosa ◽  
Bovine Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Intestinal Mucosa ◽  
Rat Jejunum ◽  
Small Intestinal

To assess the mechanisms for movement of antigenically intact macromolecules across small intestinal mucosa, transport kinetics of bovine serum albumin (BSA) uptake and the effect of neural and metabolic inhibition were examined in stripped short-circuited rat jejunum. The mucosa was exposed to BSA, and, after a 50-min equilibration, mucosal-to-serosal movement of immunologically intact BSA was determined by enzyme-linked immunosorbent assay and total BSA by radiolabeled 125I-BSA. Intact BSA uptake demonstrated saturable kinetics. Immunologically intact BSA crossed the intestinal mucosa as 4.5% of total 125I-BSA flux. Colchicine and 4 degrees C significantly reduced uptake of immunologically intact BSA. NaF significantly reduced uptake of immunologically intact BSA and 125I-BSA. Treatment with tetrodotoxin significantly reduced intact BSA uptake, but did not significantly alter total BSA uptake. The muscarinic cholinoceptor antagonist atropine also significantly inhibited transport of intact BSA, whereas the nicotinic cholinoceptor antagonist hexamethonium had no effect. These findings indicate that transport of intact macromolecules across small intestinal mucosa is a saturable energy-dependent process that utilizes the microtubular network and is regulated by the enteric nervous system primarily through cholinergic nerves acting on muscarinic receptors.

In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel

2011 ◽  
Vol 420 (2) ◽  
pp. 266-273 ◽  
Author(s):  
Kkot Nim Kang ◽  
Da Yeon Kim ◽  
So Mi Yoon ◽  
Jin Seon Kwon ◽  
Hyo Won Seo ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Small Intestinal Submucosa ◽  
In Vivo Release ◽  
Small Intestinal ◽  
Intestinal Submucosa

Lag phase alteration in the modified bovine serum albumin under the inducing and inhibitory effect of vitamin C

2018 ◽  
Vol 15 (6) ◽  
pp. 1337-1346
Author(s):  
E. Vahdat-Ahar ◽  
A. A. Moosavi-Movahedi ◽  
F. Taghavi ◽  
M. Habibi-Rezaei ◽  
N. Sheibani
Keyword(s):  
Bovine Serum Albumin ◽  
Vitamin C ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inhibitory Effect ◽  
Lag Phase

Effects of Cereal Prolamin Peptides on Differentiated CaCo-2 Cells

1996 ◽  
Vol 24 (4) ◽  
pp. 547-552
Author(s):  
Claudio Giovannini ◽  
Roberto Luchetti ◽  
Elena Mancini ◽  
Massimo de Vincenzi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Cell Viability ◽  
Mtt Assay ◽  
Bovine Serum ◽  
Cytotoxic Effect ◽  
Cell Damage ◽  
Inhibitory Effect ◽  
Culture Cell ◽  
Glycoprotein Synthesis

The effects of peptic-tiyptic (PT) digests of prolamins derived from several cereals on differentiated CaCo-2 cells were studied on the nineteenth day of culture. Cell viability was determined by using the MTT assay and the colony-forming ability method. The metabolic consequences of peptide exposure were evaluated by measuring RNA, protein and glycoprotein synthesis. While PT digests from bovine serum albumin and durum wheat did not exert any effects, those derived from bread wheat, barley, rye and oats caused a dramatic inhibitory effect on metabolic synthesis and, when measured by using the colony-forming technique, a decrease in cell viability. The MTT assay did not indicate any changes in cell viability. These observations support the hypothesis that, although prolamin-derived peptides from these cereals do not exert an immediate cytotoxic effect, they are responsible for cell damage by impairment of metabolic processes.

scholarly journals Inhibitory effect of gold nanoparticles on the D-ribose glycation of bovine serum albumin

2014 ◽  
pp. 5461 ◽  
Author(s):  
Weixi Liu ◽  
Menashi Cohenford ◽  
Leslie Frost ◽  
Champika Seneviratne ◽  
Joel Dain
Keyword(s):  
Gold Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inhibitory Effect

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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