Optimization of In Vivo Production of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

2021 ◽  
Author(s):  
Priscila Stinguel ◽  
Carlos Eduardo Costa Paiva ◽  
Vitor Zuim ◽  
Ana Clara Thezolin Azevedo ◽  
Fernando Hercos Valicente ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Multiple Nucleopolyhedrovirus

scholarly journals Spodoptera frugiperda resistance to oral infection by Autographa californica multiple nucleopolyhedrovirus linked to aberrant occlusion-derived virus binding in the midgut

2005 ◽  
Vol 86 (5) ◽  
pp. 1349-1355 ◽  
Author(s):  
Eric J. Haas-Stapleton ◽  
Jan O. Washburn ◽  
Loy E. Volkman
Keyword(s):  
Spodoptera Frugiperda ◽  
Cell Receptor ◽  
Oral Infection ◽  
Autographa Californica ◽  
Productive Infection ◽  
Receptor Interaction ◽  
Virus Binding ◽  
Multiple Nucleopolyhedrovirus ◽  
Autographa Californica Multiple Nucleopolyhedrovirus

Spodoptera frugiperda larvae are highly resistant to oral infection by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) (LD50, ∼9200 occlusions), but extremely susceptible to budded virus within the haemocoel (LD50, <1 p.f.u.). The inability of AcMNPV occlusion-derived virus (ODV) to establish primary infections readily within midgut cells accounts for a major proportion of oral resistance. To determine whether inappropriate binding of AcMNPV ODV to S. frugiperda midgut cells contributes to lack of oral infectivity, the binding and fusion properties of AcMNPV ODV were compared with those of the ODV of a new isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) obtained from a field-collected larva (oral LD50, 12 occlusions). By using a fluorescence-dequenching assay conducted in vivo, it was found that AcMNPV ODV bound to the midgut epithelia of S. frugiperda larvae at ∼15 % of the level of SfMNPV ODV, but that, once bound, the efficiencies of fusion for the two ODVs were similar: 60 % for AcMNPV and 53 % for SfMNPV. Whilst the difference in binding efficiencies was significant, it could not account entirely for the observed differences in infectivity. Competition experiments, however, revealed that, in S. frugiperda larvae, SfMNPV ODV bound to a midgut cell receptor that was not bound by AcMNPV ODV, indicating that ODV interaction with a specific receptor(s) was necessary for productive infection of midgut columnar epithelial cells. Fusion in the absence of this ligand–receptor interaction did not result in productive infections.

scholarly journals Molecular and Biological Characterization of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Field Isolate and Genotypes from China

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 777
Author(s):  
Chengfeng Lei ◽  
Jian Yang ◽  
Jia Wang ◽  
Jia Hu ◽  
Xiulian Sun
Keyword(s):  
Spodoptera Frugiperda ◽  
Field Isolate ◽  
Management Strategies ◽  
Fall Armyworm ◽  
Multiple Nucleopolyhedrovirus ◽  
Cloning Method ◽  
Occlusion Bodies ◽  
Colombian Isolate

The fall armyworm, Spodoptera frugiperda, is a new invading pest in China. The baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a pathogenic agent of the fall armyworm and a potential agent for its control in integrated pest management strategies. In this work, we analyze the molecular and biological characteristics of an SfMNPV isolate collected from maize in China (SfMNPV-Hub). Two genotypes were further isolated from SfMNPV-Hub by an in vivo cloning method. The PstI profile of one genotype (SfHub-A) was similar to genotype A of the SfMNPV Colombian isolate, and the other (SfHub-E) was similar to genotype E of the Colombian isolate. The bioactivity of SfHub-A against second-instar S. frugiperda larvae was not significantly different from that of SfMNPV-Hub, whereas SfHub-E was 2.7–5.5 fold less potent than SfMNPV-Hub. The speed of kill of SfHub-E was quicker than SfMNPV-Hub, while SfHub-A acted slower than SfMNPV-Hub. Occlusion body (OB) production of SfHub-A in an S. frugiperda cadaver was significantly higher than that of SfMNPV-Hub, while SfHub-E yielded far fewer occlusion bodies (OBs) in the host larvae. These results provide basic information for developing a virus-based pesticide against the invading pest S. frugiperda.

scholarly journals Aperfeiçoamento da Técnica de Produção Massal in vivo de Baculovírus spodoptera

2021 ◽  
Vol 14 (3) ◽  
pp. 1-13
Author(s):  
Lorena Contarini Machado ◽  
Hugo José Gonçalves dos Santos Junior ◽  
Laura Vaillant Ribeiro Mauri ◽  
José Henrique Soler Guilhen ◽  
Carlos Eduardo Costa Paiva
Keyword(s):  
Spodoptera Frugiperda ◽  
Multiple Nucleopolyhedrovirus

A multiplicação in vivo do Baculovírus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) no hospedeiro específico Spodoptera frugiperda tem como principal problema o hábito canibal dessa espécie de inseto-praga, o que aumenta custos e reduz a produção final para comercialização. Dessa forma, o objetivo desse trabalho foi aperfeiçoar o método de produção in vivo de SfMNPV baseado na densidade de lagartas e no período de multiplicação do vírus. Lagartas de S. frugiperda com 4 dias de idade foram inoculadas com suspensão viral do isolado 6 (1 x 108 OB.mL-1) de SfMNPV em dieta artificial. As lagartas foram mantidas a 31 ºC em duas densidades (50 e 100 lagartas/recipiente) e cinco períodos para multiplicação viral (48, 72, 96, 120 e 144 horas). Após cada período de multiplicação do vírus, as lagartas de ambas densidades foram congeladas, pesadas e maceradas para quantificação dos corpos de oclusão e cálculo dos parâmetros de produção. Com base nos parâmetros avaliados, as densidades de 50 e 100 lagartas/recipiente e período de 96 horas é adequado para aperfeiçoamento da produção in vivo do isolado 6 de SfMNPV em lagartas de S. frugiperda.

Virulence and Genetic Characterization of Six Baculovirus Strains Isolated From Different Populations of Spodoptera Frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Author(s):  
Ingrid Zanella-Saenz ◽  
Elisabeth A. Herniou ◽  
Jorge E. Ibarra ◽  
Ma.Cristina Del Rincón-Castro ◽  
Ilse Alejandra Huerta-Arredondo
Keyword(s):  
Biological Control ◽  
Cell Cultures ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
The United States ◽  
Infection Mechanisms ◽  
Different Populations

Abstract Fall armyworm (FAW), Spodoptera frugiperda (Smith, 1797), is a polyphagous, voracious, and economically important agricultural pest. Biological control of FAW is a strategy that must be further explored. This study evaluated six baculovirus strains isolated from infected FAW larvae from Mexico, Argentina, Honduras, and the United States. Five alphabaculoviruses (SfNPV-An2, SfNPV-Arg, SfNPV-Fx, SfNPV-Ho and SfNPV-Sin) and one betabaculovirus (SfGV-RV), were tested against FAW larvae, showing a wide diversity of virulence levels among strains when their estimated LC50s were compared, being SfNPVArg, SfNPV-Ho and SfNPV-Fx more virulent than SfNPV-An 2 , SfNPV-Sin and SfGV-RV. To determine any virulence difference in vitro studies of these isolates, Sf9 cell cultures were used. Interestingly, only ODVs from four of the test SfNPV strains showed infectivity on Sf9 cell cultures, and some differences in virulence were observed. Genomic restriction analyses and partial sequences of lef-8, lef-9 , and polh/granulin genes showed little variability among alphabaculoviruses, both, among them and with previously reported sequences. However, sequences from SfGV-RV were closer to previously reported sequences from the SfGVVG008 strain than the SfGV-Arg and SfGV-VG014 strains. The great difference in the in vivo virulence was not correlated with great similarity among the isolates. The characterization of these six baculoviruses isolates offers the basis for exploring their potential as biological control agents against S. frugiperda, as well the initial studies on their specific infection mechanisms, evolution, and ecology.

scholarly journals Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies

2008 ◽  
Vol 82 (16) ◽  
pp. 7897-7904 ◽  
Author(s):  
Oihane Simón ◽  
Trevor Williams ◽  
Aaron C. Asensio ◽  
Sarhay Ros ◽  
Andrea Gaya ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Gene Deletion ◽  
Particle Content ◽  
Reading Frame ◽  
Virus Particles ◽  
Multiple Nucleopolyhedrovirus ◽  
Glycosylation Sites ◽  
Occlusion Bodies ◽  
Viral Factor ◽  
Positive Controls

ABSTRACT The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were equally infectious when used to transfect S. frugiperda. All three viruses produced similar numbers of OBs, although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with Sf29null bacmid died ∼24 h later than positive controls, consistent with the reduced virus particle content of Sf29null OBs. Transcripts from Sf29 were detected in infected insects 12 h prior to those from the polyhedrin gene. Homologs to Sf29 were present in other group II NPVs, and similar sequences were present in entomopoxviruses. Analysis of the Sf29 predicted protein sequence revealed signal peptide and transmembrane domains, but the presence of 12 potential N-glycosylation sites suggest that it is not an ODV envelope protein. Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions. We conclude that Sf29 is a viral factor that determines the number of ODVs occluded in each OB.

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

2019 ◽  
Vol 144 (1-2) ◽  
pp. 123-132
Author(s):  
Magali Ordóñez‐García ◽  
Claudio Rios‐Velasco ◽  
José de Jesús Ornelas‐Paz ◽  
Juan Carlos Bustillos‐Rodríguez ◽  
Carlos Horacio Acosta‐Muñiz ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Spodoptera Frugiperda ◽  
Morphological Characterization ◽  
Multiple Nucleopolyhedrovirus ◽  
Lepidoptera Noctuidae

scholarly journals An Anticarsia gemmatalis multiple nucleopolyhedrovirus mutant, vApAg, induces hemocytes apoptosis in vivo and displays reduced infectivity in larvae of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae)

2007 ◽  
Vol 130 (1-2) ◽  
pp. 182-192 ◽  
Author(s):  
Eni Braga da Silveira ◽  
Bruno Arrivabene Cordeiro ◽  
Bergmann Morais Ribeiro ◽  
Maria Elita Batista de Castro ◽  
Elisa Filgueiras Soares ◽  
...  
Keyword(s):  
Anticarsia Gemmatalis ◽  
Multiple Nucleopolyhedrovirus ◽  
Lepidoptera Noctuidae

scholarly journals Toxicity, Binding, and Permeability Analyses of FourBacillus thuringiensis Cry1 δ-Endotoxins Using Brush Border Membrane Vesicles of Spodoptera exigua and Spodoptera frugiperda

1999 ◽  
Vol 65 (2) ◽  
pp. 457-464 ◽  
Author(s):  
Ke Luo ◽  
David Banks ◽  
Michael J. Adang
Keyword(s):  
Binding Site ◽  
Spodoptera Frugiperda ◽  
Brush Border ◽  
Spodoptera Exigua ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Binding Assays ◽  
Competition Binding

ABSTRACT The binding and pore formation properties of four Bacillus thuringiensis Cry1 toxins were analyzed by using brush border membrane vesicles from Spodoptera exigua andSpodoptera frugiperda, and the results were compared to the results of toxicity bioassays. Cry1Fa was highly toxic and Cry1Ac was nontoxic to S. exigua and S. frugiperda larvae, while Cry1Ca was highly toxic to S. exigua and weakly toxic to S. frugiperda. In contrast, Cry1Bb was active against S. frugiperda but only marginally active against S. exigua. Bioassays performed with iodinated Cry1Bb, Cry1Fa, and Cry1Ca showed that the effects of iodination on toxin activity were different. The toxicities of I-labeled Cry1Bb and Cry1Fa against Spodoptera species were significantly less than the toxicities of the unlabeled toxins, while Cry1Ca retained its insecticidal activity when it was labeled with 125I. Binding assays showed that iodination prevented Cry1Fa from binding to Spodoptera brush border membrane vesicles. 125I-labeled Cry1Ac, Cry1Bb, and Cry1Ca bound with high-affinities to brush border membrane vesicles fromS. exigua and S. frugiperda. Competition binding experiments performed with heterologous toxins revealed two major binding sites. Cry1Ac and Cry1Fa have a common binding site, and Cry1Bb, Cry1C, and Cry1Fa have a second common binding site. No obvious relationship between dissociation of bound toxins from brush border membrane vesicles and toxicity was detected. Cry1 toxins were also tested for the ability to alter the permeability of membrane vesicles, as measured by a light scattering assay. Cry1 proteins toxic to Spodoptera larvae permeabilized brush border membrane vesicles, but the extent of permeabilization did not necessarily correlate with in vivo toxicity.

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