scholarly journals Microbial community analysis for aerobic granular sludge reactor treating high-level 4-chloroaniline wastewater

2013 ◽  
Vol 11 (7) ◽  
pp. 1845-1854 ◽  
Author(s):  
L. Zhu ◽  
X. Dai ◽  
X. Xu ◽  
M. Lv ◽  
D. Cao
Keyword(s):  
Microbial Community ◽  
Granular Sludge ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Aerobic Granular Sludge ◽  
High Level

scholarly journals Microbial Community Shift and Role of Bacteria in Rapid Granulation By Using Diatomite

2022 ◽  
Author(s):  
Hazlami Fikri Basri ◽  
Aznah Nor Anuar ◽  
Mohd Hakim Abdul Halim
Keyword(s):  
Microbial Community ◽  
Alpha Diversity ◽  
Granular Sludge ◽  
Community Analysis ◽  
Shannon Index ◽  
Microbial Community Analysis ◽  
Aerobic Granular Sludge ◽  
Granulation Process ◽  
Community Shift ◽  
Seed Sludge

Abstract Aerobic granular sludge (AGS) technology is a promising biological method for modern wastewater treatment. However, granulation time have become a major issue for the application of AGS technology especially in low strength wastewater. Recent studies on granulation are focusing towards rapid start-up granulation process. Diatomite, a friable light-coloured sedimentary rock was introduces in this study to enhanced the granules formation. This study highlight the effect of diatomite towards the microbial community during the transformation of seed sludge until development of granules. DNA extraction and Metagenomic analysis was conducted with three samples (seed sludge, control AGS, AGS diatomite) to compare the microbial community. The microbial community analysis revealed the alpha diversity, phylum and class level, and the abundance of EPS producing bacteria of each bacteria samples respectively. Diatomite has a significant influence towards the microbial diversity (High Shannon index alpha diversity). Also, diatomite promotes the abundance of functional bacteria especially EPS producing bacteria, which seen as a crucial elements in granulation process.

Bioaugmentation of Aerobic Granular Sludge with the Addition of a Bioflocculant-Producing Consortium

2013 ◽  
Vol 726-731 ◽  
pp. 2530-2535 ◽  
Author(s):  
Zhi Wei Song ◽  
Ting Ting Ning ◽  
Yu Ping Chen ◽  
Xiao Xia Cheng ◽  
Nan Qi Ren
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Bacterial Species ◽  
Granular Sludge ◽  
Extracellular Polysaccharide ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Aerobic Granular Sludge ◽  
Gradient Gel Electrophoresis ◽  
The Stability

A consortium of bioflocculant-producing bacteria (BPA), mainly consisting of previously enriched Devosia hwasunensis and Tetrasphaera elongata, was inoculated into a sequencing batch airlift reactor during aerobic granular sludge cultivation to determine the effects of BPA on the formation of aerobic granular sludges. The results indicate that granulation time was substantially shortened from 56 to 28 days with the addition of BPA. Microbial community analysis of granular sludge based on denaturing gradient gel electrophoresis revealed diversity. Dominant populations belonged to Actinobacteria and α-and γ-proteobacteria. The added bacterial species, D. hwasunensis and T. elongata, which have been proposed to secrete extracellular polysaccharide mucus, played an important role in particle formation and in the maintenance of the stability and physicochemical properties of the granular sludge.

scholarly journals The effects of high salinity on nitrogen removal and the formation characteristics of aerobic granular sludge

2019 ◽  
Vol 25 (5) ◽  
pp. 659-667 ◽  
Author(s):  
HyunGu Kim ◽  
DaeHee Ahn
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Biological Treatment ◽  
High Salinity ◽  
Granular Sludge ◽  
Suspended Solid ◽  
Microbial Community Analysis ◽  
Volume Index ◽  
Aerobic Granular Sludge ◽  
Mixed Liquor

This study investigated the biological treatment of high-salinity wastewater using aerobic granular sludge (AGS). The removal performance, AGS characteristics, and microbial community were examined under salinity conditions ranging from 0.5 to 3.0 wt.%. When the salinity was increased, the removal efficiency of the chemical oxygen demand did not change substantially. The mean removal efficiency of NH<sub>4</sub><sup>+</sup>-N ranged from 43.7 to 72.6% during the operation period. As for the concentration of extracellular polymeric substances (EPS), the polysaccharide/protein (PS/PN) ratio increased from 0.37 to 0.57. The concentrations of the mixed liquor suspended solid (MLSS) and mixed liquor volatile suspended solid (MLVSS) were 4,560 and 3,170 mg/L, respectively, in the end phase, and the MLVSS/MLSS ratio decreased from 0.81 to 0.69. The sludge volume index (SVI<sub>30</sub>) decreased linearly from 88 to 58 mL/g. In the microbial community analysis, <i>Nitrosomonas</i> species accounted for 22.9% of the total bacteria in the initial phase, whereas <i>Halomonas</i> accounted for 43.2% in the end phase. Hence, the gradually increased salinity appears to facilitate the dominance of halophilic microorganisms in the long-term operation of an AGS-based biological treatment system and the stable retention of AGS.

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