16S rDNA PCR for the aetiological diagnosis of culture-negative infective endocarditis

Infection ◽  
2021 ◽  
Author(s):  
Vanesa Anton-Vazquez ◽  
Rafal Dworakowski ◽  
Antonio Cannata ◽  
George Amin-Youssef ◽  
Margaret Gunning ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
16S Rdna ◽  
16S Rdna Pcr ◽  
Aetiological Diagnosis ◽  
Culture Negative

scholarly journals Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1372
Author(s):  
Raquel Rodríguez-García ◽  
María Ángeles Rodríguez-Esteban ◽  
Jonathan Fernández-Suárez ◽  
Ana Morilla ◽  
Enrique García-Carús ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
16S Rdna ◽  
Heart Valve ◽  
Heart Tissue ◽  
Blood Cultures ◽  
Causative Pathogen ◽  
Complementary Information ◽  
Skin Microbiota ◽  
16S Rdna Pcr ◽  
Culture Negative

Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative.

scholarly journals The diagnostic benefit of 16S rDNA PCR examination of infective endocarditis heart valves: a cohort study of 146 surgical cases confirmed by histopathology

2020 ◽  
Author(s):  
Christina Armstrong ◽  
Tim Christian Kuhn ◽  
Matthias Dufner ◽  
Philipp Ehlermann ◽  
Stefan Zimmermann ◽  
...  
Keyword(s):  
Cohort Study ◽  
Infective Endocarditis ◽  
16S Rdna ◽  
Heart Valves ◽  
16S Rdna Pcr ◽  
Diagnostic Benefit

scholarly journals Utility of a Direct 16S rDNA PCR and Sequencing for Etiological Diagnosis of Infective Endocarditis

2017 ◽  
Vol 37 (6) ◽  
pp. 505-510 ◽  
Author(s):  
Min-Sun Kim ◽  
Jeonghyun Chang ◽  
Mi-Na Kim ◽  
Sang-Ho Choi ◽  
Sung-Ho Jung ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
16S Rdna ◽  
Etiological Diagnosis ◽  
16S Rdna Pcr

DETECTING MYCOBACTERIUM TUBERCULOSIS RAPIDLY AND MYCOBACTERIUM TUBERCULOSIS STRAINS WITHOUT IS6110 SEQUENCE BY PCR ASSAY

2012 ◽  
pp. 15-19
Author(s):  
Thi Chau Anh Nguyen ◽  
Hoang Bach Nguyen ◽  
Hai Duong Huynh ◽  
Nu Xuan Thanh Le ◽  
Xuan Cuong Le ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
16S Rdna ◽  
False Negative ◽  
Clinical Samples ◽  
Tuberculosis Complex ◽  
Pcr Assay ◽  
Complex Objects ◽  
Method Performance ◽  
Gene Coding ◽  
16S Rdna Pcr

Background: The Nested IS6110 PCR is used for detecting tuberculosis, however IS6110 sequence is not present in the genome of all strains of M.tuberculosis, the result may be false negative. The gene coding 16S ribosome always contains a short sequence specific to M. tuberculosis complex. Objects: Performance of the 16S Real-time PCR to detect M. tuberculosis and combining to the nested IS6110 PCR to determine the rate of Mtb strains without IS6110 from clinical samples. Materials and method: Performance of 16S rDNA PCR by commercial kit of Viet A Inc. for all 480 samples, the samples which were positive with the 16S rDNA PCR were retested in IS6110 PCR assay by in-house kit. Results: The Realtime 16S rDNA PCR detected 258 cases (53.8%) of tuberculosis. There were 3 (1.2 %) M. tuberculosis strains which do not harbor IS6110 sequence in genome. Conclusion: The IS6110 nested PCR can be applied more widely than the 16S rDNA realtime PCR. In case of using IS6110 PCR assay, results may show a low proportion of false negative. Combining 16S rDNA PCR with the IS6110 based PCR allowed detection of deletion of IS6110 sequence in M. tuberculosis isolates.

Blood culture-negative infective endocarditis: a worse outcome? Results from a large multicentre retrospective Spanish cohort study

2021 ◽  
pp. 1-9
Author(s):  
Lorenzo Roberto Suardi ◽  
Arístides de Alarcón ◽  
María Victoria García ◽  
Antonio Plata Ciezar ◽  
Carmen Hidalgo Tenorio ◽  
...  
Keyword(s):  
Cohort Study ◽  
Infective Endocarditis ◽  
Blood Culture ◽  
Culture Negative ◽  
Spanish Cohort

Isolation of Enterobacter sakazakii from Stable Flies, Stomoxys calcitrans L. (Diptera: Muscidae)†

2006 ◽  
Vol 69 (3) ◽  
pp. 671-673 ◽  
Author(s):  
F. MRAMBA ◽  
A. BROCE ◽  
L. ZUREK
Keyword(s):  
16S Rdna ◽  
Foodborne Pathogen ◽  
Animal Manure ◽  
Stomoxys Calcitrans ◽  
Fecal Coliforms ◽  
Stable Fly ◽  
Enterobacter Sakazakii ◽  
Stable Flies ◽  
16S Rdna Pcr ◽  
Glucose Agar

Enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. Previously, it was suggested that stable flies, Stomoxys calcitrans, were a vector or reservoir of this pathogen. In our study, by means of a culturing approach combined with 16S rDNA PCR–restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in Kansas and Florida. Two stable flies (0.2%) were positive for E. sakazakii. In addition, 411 (44%) stable flies carried bacteria-forming red colonies (presumably enterics) on a violet red bile glucose agar (mean count = 6.4 × 104 CFU per fly), and 120 (13%) stable flies carried fecal coliforms (mean count = 8.7 × 103 CFU per fly). Sequencing of 16S rDNA showed that enterics from violet red bile glucose agar were represented by several genera, including Escherichia, Shigella, Providencia, Enterobacter, Pantoea, Proteus, Serratia, and Morganella. Our study shows that stable flies carry bacteria typically present in animal manure (a developmental site of stable fly larvae), which indicates that the natural reservoir of E. sakazakii is the digestive tract or manure of domestic animals. The low prevalence of E. sakazakii associated with stable flies suggests that stable flies do not play a major role as a reservoir or vector of this pathogen.

scholarly journals Identification of Leptotrichia goodfellowii Infective Endocarditis by Next-Generation Sequencing of 16S rDNA Amplicons

2020 ◽  
pp. mcs.a005876
Author(s):  
Joshua Lieberman ◽  
Kyoko Kurosawa ◽  
Dhruba J SenGupta ◽  
Brad T Cookson ◽  
Stephen J Salipante ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Infective Endocarditis ◽  
16S Rdna ◽  
Next Generation ◽  
Generation Sequencing
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