Investigation of expression of myocardial miR-126, miR-29a and miR-222 as a potential marker in STZ- induced diabetic rats following interval and continuous exercise training

Interval training (IT), consisting of alternated periods of high and low intensity exercise, has been proposed as a strategy to induce more marked biological adaptations than continuous exercise training (CT). The purpose of this study was to assess the effects of IT and CT with equivalent total energy expenditure on capillary skeletal and cardiac muscles in rats. Wistar rats ran on a treadmill for 30 min per day with no slope (0%), 4 times/week for 13 weeks. CT has constant load of 70% max; IT has cycles of 90% max for 1 min followed by 1 min at 50% max. CT and IT increased endurance and muscle oxidative capacity and attenuated body weight gain to a similar extent (P>0.05). In addition, CT and IT similarly increased functional capillary density of skeletal muscle (CT:30.6±11.7%; IT:28.7±11.9%) and the capillary-to-fiber ratio in skeletal muscle (CT:28.7±14.4%; IT:40.1±17.2%) and in the left ventricle (CT:57.3±53.1%; IT:54.3±40.5%). In conclusion, at equivalent total work volumes, interval exercise training induced similar functional and structural alterations in the microcirculation of skeletal muscle and myocardium in healthy rats compared to continuous exercise training.

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TREADMILL EXERCISE TRAINING PROTECTS AGAINST METABOLIC DYSFUNCTION AND DIAPHRAGM WEAKNESS IN OBESE DIABETIC RATS

The FASEB Journal ◽

10.1096/fasebj.2018.32.1_supplement.588.26 ◽

2018 ◽

Vol 32 (S1) ◽

Author(s):

Noriko Ichinoseki‐Sekine ◽

Takamasa Tsuzuki ◽

Matthew J. Hinkley ◽

Toshinori Yoshihara ◽

Hiroyuki Kobayashi ◽

...

Keyword(s):

Exercise Training ◽

Treadmill Exercise ◽

Diabetic Rats ◽

Metabolic Dysfunction ◽

Obese Diabetic Rats ◽

Diaphragm Weakness

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Influence of exercise training on ischemic brain injury in type 1 diabetic rats

Journal of Applied Physiology ◽

10.1152/japplphysiol.00437.2012 ◽

2012 ◽

Vol 113 (7) ◽

pp. 1121-1127 ◽

Cited By ~ 16

Author(s):

Denise M. Arrick ◽

Hong Sun ◽

William G. Mayhan

Keyword(s):

Brain Injury ◽

Exercise Training ◽

Vascular Function ◽

Ischemia Reperfusion ◽

Diabetic Rats ◽

Ischemic Brain Injury ◽

Ischemic Brain ◽

Cerebrovascular Function ◽

Pial Arterioles

While exercise training (ExT) appears to influence cerebrovascular function during type 1 diabetes (T1D), it is not clear whether this beneficial effect extends to protecting the brain from ischemia-induced brain injury. Thus our goal was to examine whether modest ExT could influence transient focal ischemia-induced brain injury along with nitric oxide synthase (NOS)-dependent dilation of cerebral (pial) arterioles during T1D. Sprague-Dawley rats were divided into four groups: nondiabetic sedentary, nondiabetic ExT, diabetic (streptozotocin; 50 mg/kg ip) sedentary, and diabetic ExT. In the first series of studies, we measured infarct volume in all groups of rats following right MCA occlusion for 2 h, followed by 24 h of reperfusion. In a second series of studies, a craniotomy was performed over the parietal cortex, and we measured responses of pial arterioles to an endothelial NOS (eNOS)-dependent, a neuronal NOS (nNOS)-dependent, and a NOS-independent agonist in all groups of rats. We found that sedentary diabetic rats had significantly larger total, cortical, and subcortical infarct volumes following ischemia-reperfusion than sedentary nondiabetic, nondiabetic ExT, and diabetic ExT rats. Infarct volumes were similar in sedentary nondiabetic, ExT nondiabetic, and ExT diabetic rats. In contrast, ExT did not alter infarct size in nondiabetic compared with sedentary nondiabetic rats. In addition, ExT diabetic rats had impaired eNOS- and nNOS-dependent, but not NOS-independent, vasodilation that was restored by ExT. Thus ExT of T1D rats lessened ischemic brain injury following middle cerebral artery occlusion and restored impaired eNOS- and nNOS-dependent vascular function. Since the incidence of ischemic stroke is increased during T1D, we suggest that our finding are significant in that modest ExT may be a viable preventative therapeutic approach to lessen ischemia-induced brain injury that may occur in T1D subjects.

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Interval versus continuous exercise training in coronary artery disease: a meta-analysis

European Heart Journal ◽

10.1093/eurheartj/eht308.1963 ◽

2013 ◽

Vol 34 (suppl 1) ◽

pp. 1963-1963

Author(s):

E. Coeckelberghs ◽

N. Pattyn ◽

R. Buys ◽

V. A. Cornelissen ◽

L. Vanhees

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Exercise Training ◽

Meta Analysis ◽

Continuous Exercise ◽

Artery Disease

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Influence of training on skeletal muscle enzymatic adaptations in normal and diabetic rats

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1985.249.4.e360 ◽

1985 ◽

Vol 249 (4) ◽

pp. E360-E365 ◽

Cited By ~ 2

Author(s):

E. G. Noble ◽

C. D. Ianuzzo

Keyword(s):

Skeletal Muscle ◽

Exercise Training ◽

Citrate Synthase ◽

Diabetic Rats ◽

Muscle Fiber Types ◽

Fiber Types ◽

Marker Enzymes ◽

Oxidative Potential ◽

Hydroxyacyl Coa Dehydrogenase ◽

Fast Twitch

Muscle homogenates representing slow-twitch oxidative, fast-twitch oxidative-glycolytic, fast-twitch glycolytic, and mixed fiber types were prepared from normal, diabetic, and insulin-treated diabetic rats. Diabetes was induced by injection of 80 mg . kg-1 of streptozotocin. The activities of citrate synthase, succinate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase were employed as markers of oxidative potential, whereas phosphorylase, hexokinase, and phosphofructokinase activities were used as an indication of glycolytic capacity. Diabetes was associated with a general decrement in the activity of oxidative marker enzymes for all fiber types except the fast-twitch glycolytic fiber. In contrast, the fast-twitch glycolytic fibers demonstrated the greatest decline in glycolytic enzymatic activity. Insulin-treated animals, either trained or untrained, exhibited enzyme activities similar to their normal counterparts. Exercise training of diabetic rats mimicked the effect of insulin treatment and caused a near normalization of the activity of the marker enzymes. These findings suggest that the enzymatic potential of all skeletal muscle fiber types of diabetic rats may be normalized by exercise training even in the absence of significant amounts of insulin.

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Exercise training alleviates MCT1 and MCT4 reductions in heart and skeletal muscles of STZ-induced diabetic rats

Journal of Applied Physiology ◽

10.1152/japplphysiol.01155.2002 ◽

2003 ◽

Vol 94 (6) ◽

pp. 2433-2438 ◽

Cited By ~ 29

Author(s):

Taisuke Enoki ◽

Yuko Yoshida ◽

Hideo Hatta ◽

Arend Bonen

Keyword(s):

Skeletal Muscle ◽

Exercise Training ◽

Skeletal Muscles ◽

Diabetic Rats ◽

Monocarboxylate Transporter ◽

Sedentary Control ◽

Monocarboxylate Transporter 1

We compared the changes in monocarboxylate transporter 1 (MCT1) and 4 (MCT4) proteins in heart and skeletal muscles in sedentary control and streptozotocin (STZ)-induced diabetic rats (3 wk) and in trained (3 wk) control and STZ-induced diabetic animals. In nondiabetic animals, training increased MCT1 in the plantaris (+51%; P < 0.01) but not in the soleus (+9%) or the heart (+14%). MCT4 was increased in the plantaris (+48%; P < 0.01) but not in the soleus muscles of trained nondiabetic animals. In sedentary diabetic animals, MCT1 was reduced in the heart (−30%), and in the plantaris (−31%; P < 0.01) and soleus (−26%) muscles. MCT4 content was also reduced in sedentary diabetic animals in the plantaris (−52%; P < 0.01) and soleus (−25%) muscles. In contrast, in trained diabetic animals, MCT1 and MCT4 in heart and/or muscle were similar to those of sedentary, nondiabetic animals ( P > 0.05) but were markedly greater than in the sedentary diabetic animals [MCT1: plantaris +63%, soleus +51%, heart +51% ( P > 0.05); MCT4: plantaris +107%, soleus +17% ( P > 0.05)]. These studies have shown that 1) with STZ-induced diabetes, MCT1 and MCT4 are reduced in skeletal muscle and/or the heart and 2) exercise training alleviated these diabetes-induced reductions.

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