Effect of Dietary Vitamin D3 and Ultraviolet B Light on Growth Performance, Blood Serum Parameters, Gut Histology, and Welfare Indicators of Broilers

Stressors are commonly encountered by all farmed species, including chickens, but the impact of these stressors on the animal and their productivity can be influenced by the environmental conditions in which they are kept. This study investigated the effects of dietary vitamin D3 (vitD3) and ultraviolet light (UVB) on growth performance, organ weight, serum corticosterone levels (CORT), serum 25-hydroxy vitamin D (25-OH-D3) status, gut histology, and welfare indicators of broiler chickens challenged with social isolation stress. One day (d) old Ross 308 broiler chicks (n = 192) were individually weighed, wing-tagged, and allocated to non-isolated (control) and isolated groups; control birds were never isolated, while isolated birds were subjected to regular sessions of social isolation for about 15-min periods over the course of 3 d a week for 2 weeks starting from d 10 (1.30 h total exposure) with inter treatment interval of 48 h. Birds were treated with either dietary vitD3 at 4,000 IU/kg (HD) or UVB light (UVB). The UVB lamp (24 Watt 12% UVB D3, 55 cm) with wavelength: 280–315 nm, intensity; 28.12 μW/cm2 hung 50 cm above the substrate was used for the broilers in all the treatment groups but were filtered to remove UVB in the HD group. Growth performance measure; body weight gain, feed intake, and feed conversion ratio were estimated at the end of starter (day 10), grower (day 24), and finisher periods (day 38). Broilers were feather and gait scored to measure welfare at 22/35 and 24/37 days of age, respectively. The selected birds were weighed and euthanized to obtain serum to determine 25-OH-D3 and CORT levels, GIT weights, and gut histology. Subjecting the birds to 2-week social isolation (for 15 min, three times per week) increased CORT levels but did not alter GP and 25-OH-D3 levels of broilers. However, UVB-treated broilers demonstrated better welfare, duodenal absorptive capacity, and reduced FCR compared to HD chickens. Results suggest some beneficial effects of UVB lighting on welfare indicators and the potential to support early life growth of commercial broilers reared indoors, which are often challenged with stressors.

A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome

To identify possible mechanisms by which maternal consumption of non-nutritive sweeteners increases obesity risk in offspring, we reconstructed the major alterations in the cecal microbiome of 3-week-old offspring of obese dams consuming high fat/sucrose (HFS) diet with or without aspartame (5–7 mg/kg/day) or stevia (2–3 mg/kg/day) by shotgun metagenomic sequencing (n = 36). High throughput 16S rRNA gene sequencing (n = 105) was performed for dams, 3- and 18-week-old offspring. Maternal consumption of sweeteners altered cecal microbial composition and metabolism of propionate/lactate in their offspring. Offspring daily body weight gain, liver weight and body fat were positively correlated to the relative abundance of key microbes and enzymes involved in succinate/propionate production while negatively correlated to that of lactose degradation and lactate production. The altered propionate/lactate production in the cecum of weanlings from aspartame and stevia consuming dams implicates an altered ratio of dietary carbohydrate digestion, mainly lactose, in the small intestine vs. microbial fermentation in the large intestine. The reconstructed microbiome alterations could explain increased offspring body weight and body fat. This study demonstrates that intense sweet tastants have a lasting and intergenerational effect on gut microbiota, microbial metabolites and host health.

Comparison of growth performance among channel-blue hybrid catfish, ccGH transgenic channel catfish, and channel catfish in a tank culture system

Fish is an essential source of high-quality protein for people worldwide. The present study was designed to compare the growth performance among the channel-blue hybrid catfish, channel catfish transgenic for the channel catfish growth hormone (ccGH) cDNA driven by the antifreeze protein promoter from an ocean pout Zoarces americanus (opAFP-ccGH), and non-transgenic channel catfish control. Mean body weight of channel-blue hybrid catfish was 15.80 and 24.06% larger than non-transgenic channel catfish control at 4 and 18 months of age, respectively. However, transgenic opAFP-ccGH channel catfish were 5.52 and 43.41% larger than channel-blue hybrid catfish and 22.19 and 77.91% larger than their controls at 4 and 18 months of age, respectively. Significant differences in mean body weight between the sexes within all genetic types were found. Males were larger than females (P < 0.001). However, mean body weight of non-transgenic males was not larger than transgenic opAFP-ccGH females or male and female hybrid catfish. Condition factor of transgenic opAFP-ccGH channel catfish was higher (P < 0.05) than that of full-sibling, non-transgenic channel catfish and hybrid catfish. The mean percentage body weight gain of GH transgenic channel catfish was 559%, the channel-blue hybrid catfish was 384.9% and their non-transgenic controls channel catfish was 352.6%.

Gene-Set Enrichment Analysis for Identifying Genes and Biological Activities Associated with Growth Traits in Dromedaries

Growth is an important heritable economic trait for dromedaries and necessary for planning a successful breeding program. Until now, genome-wide association studies (GWAS) and QTL-mapping have identified significant single nucleotide polymorphisms (SNPs) associated with growth in domestic animals, but in dromedaries, the number of studies is very low. This project aimed to find biological themes affecting growth in dromedaries. In the first step, 99 candidate SNPs were chosen from a previously established set of SNPs associated with body weight, gain, and birth weight in Iranian dromedaries. Next, 0.5 kb upstream and downstream of each candidate SNP were selected from NCBI (assembly accession: GCA_000803125.3). The annotation of fragments with candidate SNPs regarding the reference genome was retrieved using the Blast2GO tool. Candidate SNPs associated with growth were mapped to 22 genes, and 25 significant biological themes were identified to be related to growth in dromedaries. The main biological functions included calcium ion binding, protein binding, DNA-binding transcription factor activity, protein kinase activity, tropomyosin binding, myosin complex, actin-binding, ATP binding, receptor signaling pathway via JAK-STAT, and cytokine activity. EFCAB5, MTIF2, MYO3A, TBX15, IFNL3, PREX1, and TMOD3 genes are candidates for improving growth in camel breeding programs.

Pasture Height of A Tropical Erect Grass Affecting Young Lamb Herbage Intake And Performance

Tropical erect grass pastures have high forage production potential in subtropical and tropical regions of the world. However, in this kind of pasture, the body weight gain of weaned lambs is usually below of their potential. We determined the effect of pasture height of an erect tropical grass, consequently its structure, on intake and performance of young lambs. The experiment was repeated in two years. Thirty young weaned lambs (4-5 months) were assigned, each year, to three Panicum maximum pasture heights: 1) Tall-75 cm; 2) Medium-50 cm and 3) Short-25 cm. Herbage mass, pasture height, plant morphological composition, pasture nutritional quality, lamb’s average daily gain, gain per hectare and herbage intake were measured. The experiment was installed in a completely randomized design, evaluated in two periods each year. The Short treatment presented, on average and for longer, the highest leaf:stem ratio (1.3 ± 0.23) and average daily gain (91 ± 10 g/day) in relation to the Medium and Tall treatments (68 ± 10 and 40 ± 13 g, respectively). There was a significant interaction between treatment*period for herbage intake. The Tall treatment showed a marked decrease in intake from the first to the second period. The pasture height management interferes in the speed at which the number of stems increases of a tropical erect grass, in relation to leaves. For a better performance of weaned young lambs during summer-autumn period, it is important to manage tropical erect grass pastures at lower height than is generally recommended, lower than 25 cm.

Pituitary adenylate cyclase-activating polypeptide receptor activation in the hypothalamus recruits unique signaling pathways involved in energy homeostasis

Pituitary adenylate cyclase activating polypeptide (PACAP) exerts pleiotropic effects on ventromedial nuclei (VMN) of the hypothalamus and its control of feeding and energy expenditure through the Type I PAC1 receptor. However, the endogenous role of PAC1R's in the VMN and the downstream signaling responsible for PACAP's effects on energy balance are unknown. Numerous studies have revealed that PAC1Rs are coupled to both Gas/ adenylate cyclase/protein kinase A (Gas/AC/PKA) and Gaq/phospholipase C/protein kinase C (Gaq/PLC/PKC), while also undergoing trafficking following stimulation. To determine the endogenous role PAC1R's and downstream signaling that may explain PACAP's pleiotropic effects, we used RNA interference to knockdown VMN PAC1Rs and pharmacologically inhibited PKA, PKC and PAC1R trafficking. Knocking down PAC1Rs increased meal sizes, reduced total number of meals, and induced body weight gain. Inhibition of either PKA or PKC alone in awake male Sprague Dawley rats, attenuated PACAP's hypophagic and anorectic effects during the dark phase. However, PKA or PKC inhibition potentiated PACAP's thermogenic effects during the light phase. Analysis of locomotor activity revealed that PKA inhibition augmented PACAP's locomotor effects, however, PKC inhibition had no effect. Finally, PACAP infusion in the VMN induces surface PAC1R trafficking into the cytosol which was blocked by endocytosis inhibitors. Subsequently, inhibition of PAC1R trafficking into the cytosol attenuated PACAP-induced hypophagia. These results revealed that endogenous PAC1Rs uniquely engage PKA, PKC and receptor trafficking to mediate PACAP's pleiotropic effects in VMN control of feeding and metabolism.

Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice

The effects of synbiotic yogurt supplemented with inulin on the pathological manifestations and gut microbiota–bile acid axis were investigated using a dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) mice model. Female C57BL/6J mice were injected subcutaneously with DHEA at a dose of 6 mg/100 g BW for 20 days to establish a PCOS mouse model. Then, the PCOS mice were treated with yogurt containing inulin (6% w/w) at 15 mL/kg BW for 24 days. Results showed that supplementation of synbiotic yogurt enriched with inulin to PCOS mice decreased the body weight gain, improved estrus cycles and ovary morphology, and reduced the levels of luteinizing hormone while increasing the levels of follicle-stimulating hormone and interleukin-22 in serum. At the genus level, synbiotic yogurt increased the relative abundance of Lactobacillus, Bifidobacterium, and Akkermansia. PICRUSt analysis indicated that KEGG pathways including bile acid biosynthesis were changed after inulin-enriched synbiotic yogurt supplementation. Synbiotic yogurt enriched with inulin also modulated the bile acid profiles. In conclusion, inulin-enriched synbiotic yogurt alleviated reproductive dysfunction and modulated gut microbiota and bile acid profiles in PCOS mice.

METHOD TO IMPROVE THE CONSUMER QUALITY AND ENVIRONMENTAL SAFETY OF QUAIL MEAT DURING DENITRIFICATION

Phospholipids are a kind of “solvents” of cholesterol and show a hepatoprotective effect. Also, phospholipids regulate fat and carbohydrate metabolism, improve the functional activity of the liver and its detoxification function. The study aims to study the impact of different doses of phos-pholipid lecithin preparation on economically useful indicators, meat productivity, nutritional value and environmental safety of quail meat at a subtoxic dose of nitrates in their compound feed. This article presents materials that indicate the feasibility of introducing phospholipid preparation of lecithin in the amount of 1.0 % by weight of feed under the presence of a sub-toxic dose of nitrates in the compound feed quail grown for meat. This introduction of phospholipid preparation of lecithin increases the preservation of livestock, body weight gain and reduces the cost of feed per unit gain. Poultry of the 2nd experimental group had an advantage over their control counterparts in preservation rate by 4.0 % and average daily growth by 9.50 % (P<0.05). The authors observed the benefit over the control samples in the concentration of dry substances by 0.98 and 0.99 %, protein by 0.98 and 0.97 % (P<0.05) in the examples of thigh and breast muscles of the poultry of the 2nd experimental group. The value of the protein-quality index (PQI) was higher by 13,55 % (P<0,05) in the samples of white meat from poultry carcasses of the 2nd experimental group as compared with the control counterparts. Also, the examples of poultry meat of the 2nd experimental group had better sanitary and hygienic characteristics. For instance, they had 36.83 % lower nitrate content (P<0.05) and 40.91 % lower nitrites content (P<0.05). This content indicates high denitrifying properties of phospholipid lecithin.

Relationship Between Circulating Metabolic Hormones and Their Central Receptors During Ovariectomy-Induced Weight Gain in Rats

Although increasing research focuses on the phenomenon of body weight gain in women after menopause, the complexity of body weight regulation and the array of models used to investigate it has proven to be challenging. Here, we used ovariectomized (OVX) rats, which rapidly gain weight, to determine if receptors for ghrelin, insulin, or leptin in the dorsal vagal complex (DVC), arcuate nucleus (ARC), or paraventricular nucleus (PVN) change during post-ovariectomy weight gain. Female Sprague-Dawley rats with ad libitum access to standard laboratory chow were bilaterally OVX or sham OVX. Subgroups were weighed and then terminated on day 5, 33, or 54 post-operatively; blood and brains were collected. ELISA kits were used to measure receptors for ghrelin, insulin, and leptin in the DVC, ARC, and PVN, as well as plasma ghrelin, insulin, and leptin. As expected, body weight increased rapidly after ovariectomy. However, ghrelin receptors did not change in any of the areas for either group, nor did circulating ghrelin. Thus, the receptor:hormone ratio indicated comparable ghrelin signaling in these CNS areas for both groups. Insulin receptors in the DVC and PVN decreased in the OVX group over time, increased in the PVN of the Sham group, and were unchanged in the ARC. These changes were accompanied by elevated circulating insulin in the OVX group. Thus, the receptor:hormone ratio indicated reduced insulin signaling in the DVC and PVN of OVX rats. Leptin receptors were unchanged in the DVC and ARC, but increased over time in the PVN of the Sham group. These changes were accompanied by elevated circulating leptin in both groups that was more pronounced in the OVX group. Thus, the receptor:hormone ratio indicated reduced leptin signaling in the DVC and PVN of both groups, but only in the OVX group for the ARC. Together, these data suggest that weight gain that occurs after removal of ovarian hormones by ovariectomy is associated with selective changes in metabolic hormone signaling in the CNS. While these changes may reflect behavioral or physiological alterations, it remains to be determined whether they cause post-ovariectomy weight gain or result from it.