Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

2021 ◽  
Author(s):  
Warepam Amuchou Singh ◽  
Nandeibam Samarjit Singh ◽  
Elangbam Julia Devi ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi
Keyword(s):  
Issr Markers ◽  
Gene Pools ◽  
Musa Spp ◽  
Pcr Rflp ◽  
Rapd And Issr

scholarly journals Characterization of genetic structure of alfalfa (Medicago sp.) from trans-Himalaya using RAPD and ISSR markers

2011 ◽  
Vol 10 (42) ◽  
pp. 8176-8187 ◽  
Author(s):  
Raj Xavier Janifer ◽  
Kumar Jitendra ◽  
Bihari Srivastava Ravi
Keyword(s):  
Genetic Structure ◽  
Issr Markers ◽  
Rapd And Issr

Molecular characterization of jasmine genotypes using RAPD and ISSR markers

2020 ◽  
Vol 77 (1) ◽  
pp. 149
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram ◽  
Ajit Kumar
Keyword(s):  
Molecular Characterization ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals BOTANICAL AND GENETIC CHARACTERIZATION OF CITRUS MAXIMA (BURM.) MERRILL. F. RUTACEAE

2016 ◽  
Vol 9 (1) ◽  
pp. 260
Author(s):  
Mohammed S. Sedeek ◽  
Farid N. Kirollos ◽  
Camilia G. Michel ◽  
Mostafa A. Abdel Kawy
Keyword(s):  
Genetic Polymorphism ◽  
Dna Fingerprinting ◽  
Citrus Sinensis ◽  
Morphological Characters ◽  
Issr Markers ◽  
Citrus Paradisi ◽  
Citrus Maxima ◽  
Rapd And Issr ◽  
Combined Data

<p><strong>Objective: </strong>Total investigation of the macro-and micromorphological features of <em>Citrus maxima</em> (Burm.) Merrill. In addition, DNA fingerprinting in this study was to estimate genetic polymorphism and relationships with another citrus viz: <em>Citrus paradise</em><strong> </strong>and <em>Citrus sinensis</em> based on RAPD and ISSR markers.</p><p><strong>Methods: </strong>For the establishment of macro-and micromorphological investigation and genetic estimation, this study presents a total investigation of the macro-and micromorphological features of the roots, stems, leaves, flowers, fruits and seeds of the plants through microscopical investigation of the prepared entire, transverse sections and powdered forms of different organs under study. Furthermore, the DNA of the three plants was extracted from leaf samples and two types of DNA markers RAPD and ISSR were assayed.</p><p><strong>Results: </strong>Macro-and micromorphological features of different organs were investigated. On the other hand Genetic polymorphism and relationships among <em>Citrus maxima</em>, <em>Citrus paradisi</em> and <em>Citrus sinensis</em> was revealed. The highest genetic similarity was between <em>Citrus maxima </em>and <em>Citrus paradisi</em> while the lowest similarity was between <em>Citrus paradisi</em> and <em>Citrus sinensis</em> as revealed by RAPD, ISSR and combined data.</p><p><strong>Conclusion: </strong>For the present study, macro and micro-morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters for authentication of <em>Citrus maxima</em> (Burm.) Merrill.</p>

scholarly journals A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

2016 ◽  
Vol 14 (4) ◽  
pp. e0712 ◽  
Author(s):  
Isaura Castro ◽  
Olinda Pinto-Carnide ◽  
Jesús M. Ortiz ◽  
Vanessa Ferreira ◽  
Juan P. Martín
Keyword(s):  
Genetic Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characters ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Molecular Techniques ◽  
Wine Production ◽  
Rapd And Issr ◽  
Simple Sequence

Grapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of ‘Vinhos Verdes’ and ‘Douro’ Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections.

Molecular Characterization of Brinjal (Solanum melongena L) Cultivars using RAPD and ISSR Markers

2009 ◽  
Vol 18 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Shailesh K. Tiwari ◽  
J. L. Karihaloo ◽  
Nowsheen Hameed ◽  
Ambika B. Gaikwad
Keyword(s):  
Molecular Characterization ◽  
Solanum Melongena ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Molecular Characterization of Saffron-Potential Candidates for Crop Improvement

2015 ◽  
Vol 7 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Javid Iqbal MIR ◽  
Nazeer AHMED ◽  
Mudasir Hafiz KHAN ◽  
Taseem Ahmad MOKHDOMI ◽  
Sajad Hussian WANI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Significant Variation ◽  
Rapd Markers ◽  
Crop Improvement ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Improvement Programs ◽  
Rapd And Issr

In this study, thirty one (31) morphologically distinct selections of saffron crop were used for molecular characterization. Molecular characterization was done through SSR, ISSR and RAPD markers. RAPD and ISSR markers showed significant variation; however, SSR markers did not reveal any variation between the selected clones. The Jaccard’s similarity coefficient ranged from 0.94 to 1.00 with an average of 0.98 among all 31 selections used. Minimum similarity value (0.94) was observed between CITH-S-107 and PAM-S-116 selections. The study provides sufficient knowledge to identify clones with better stigma characteristics for further crop improvement programs.

Genetic characterization of micropropagated Iris germanica varieties as assessed by RAPD and ISSR markers

2013 ◽  
Vol 24 ◽  
pp. S121-S122
Author(s):  
Petolescu Cerasela ◽  
Lapadatescu Simona ◽  
Lazar Alexandru ◽  
Bala Maria
Keyword(s):  
Genetic Characterization ◽  
Issr Markers ◽  
Iris Germanica ◽  
Rapd And Issr
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