Genetic Diversity, Population Structure and Screening of Molecular Markers Associated to Agronomic Traits in Safflower (Carthamus tinctorius L.)

Author(s):  
Mehdi Rahimi
Parasitology ◽  
2002 ◽  
Vol 125 (7) ◽  
pp. S51-S59 ◽  
Author(s):  
J. CURTIS ◽  
R. E. SORENSEN ◽  
D. J. MINCHELLA

Blood flukes in the genus Schistosoma are important human parasites in tropical regions. A substantial amount of genetic diversity has been described in populations of these parasites using molecular markers. We first consider the extent of genetic variation found in Schistosoma mansoni and some factors that may be contributing to this variation. Recently, though, attempts have been made to analyze not only the genetic diversity but how that diversity is partitioned within natural populations of schistosomes. Studies with non-allelic molecular markers (e.g. RAPDs and mtVNTRs) have indicated that schistosome populations exhibit varying levels of gene flow among component subpopulations. The recent characterization of microsatellite markers for S. mansoni provided an opportunity to study schistosome population structure within a population of schistosomes from a single Brazilian village using allelic markers. Whereas the detection of population structure depends strongly on the type of analysis with a mitochondrial marker, analyses with a set of seven microsatellite loci consistently revealed moderate genetic differentiation when village boroughs were used to define parasite subpopulations and greater subdivision when human hosts defined subpopulations. Finally, we discuss the implications that such strong population structure might have on schistosome epidemiology.


2015 ◽  
Vol 15 (1) ◽  
pp. 1-11 ◽  
Author(s):  
B. Usha Kiran ◽  
N. Mukta ◽  
P. Kadirvel ◽  
K. Alivelu ◽  
S. Senthilvel ◽  
...  

Safflower is a multi-purpose oilseed crop, primarily known for good quality oil containing highest polyunsaturated fatty acid content (>80%) among edible oils. In this study, a core subset of 148 safflower accessions representing 15 countries, predominantly of Indian origin, was evaluated for agronomic traits and characterized for genetic diversity, population structure and linkage disequilibrium (LD) using 44 simple sequence repeat (SSR) loci across 11 linkage groups to enable its utilization in breeding and genetic mapping purposes. The collection had substantial variation for seed yield-related traits. SSR allelic variation was low as indicated by average number of alleles (3.6) per locus, gene diversity (0.314) and polymorphism information content (0.284). Cluster analysis (neighbour-joining tree) revealed five major genotypic groups with very low bootstrap support. STRUCTURE analysis showed recognizable population structure; based on membership coefficients ( ≥ 0.75), 52% accessions were classified into four populations (K= 4) and the remaining 48% accessions into admixture group. High Fst values (0.30–0.48) suggested that the populations were substantially differentiated. Analysis of molecular variance results showed that maximum of genetic variation (85%) was explained between individuals within the population suggesting that the population structure was only weak. About 1.9% of SSR locus pairs were in LD, which appeared to be low. High phenotypic variation, mild population structure and low level of LD among unlinked loci suggested that the core subset can be explored for association mapping of seed yield components in safflower.


Author(s):  
Indu Rialch ◽  
Rama Kalia ◽  
H. K. Chaudhary ◽  
B. Kumar ◽  
J. C. Bhandari ◽  
...  

Ten morpho-agronomic traits and 80 random amplified polymorphic DNA (RAPD) molecular markers were used to survey genetic diversity in 25 chickpea genotypes. Analysis of variance revealed significant variability among different genotypes for morpho-metric traits. The cluster analysis done using morpho-metric traits grouped 25 genotypes into seven and six clusters in Environment I (Env. I) and Environment II (Env. II), respectively. Three genotypes viz., ICCV-96904, HPG-17, ICCV-95503 and L-HR-1 belonging to diverse clusters were identified divergent and may use in heterosis breeding programme. Of 80 random RAPD markers, 25 were found polymorphic. Three major clusters were identified using 25 polymorphic RAPD markers. The genetic similarity coefficient among genotypes ranged from 0.57 to 0.91. The average polymorphic information content (PIC) for 25 RAPD markers ranges from 0.12 to 0.40. D2-statistic, RAPD analysis and study of genotypes performance revealed sufficient genetic diversity among chickpea genotypes which would be useful in future breeding programme.


2008 ◽  
Vol 2 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Zhikun Li ◽  
Xingfen Wang ◽  
Yan Zhang ◽  
Guiyin Zhang ◽  
Liqiang Wu ◽  
...  

2014 ◽  
Vol 33 (5) ◽  
pp. 1299-1313 ◽  
Author(s):  
Shivendra Kumar ◽  
Heena Ambreen ◽  
T. V. Murali ◽  
Sapinder Bali ◽  
Manu Agarwal ◽  
...  

2012 ◽  
Vol 298 (9) ◽  
pp. 1701-1710 ◽  
Author(s):  
Maria Luisa Savo Sardaro ◽  
Maroun Atallah ◽  
Maurizio Enea Picarella ◽  
Benedetto Aracri ◽  
Mario A. Pagnotta

2010 ◽  
Vol 60 (4) ◽  
pp. 203-210 ◽  
Author(s):  
M. G. Ásbjarnardóttir ◽  
T. Kristjánsson ◽  
M. B. Jónsson ◽  
J. H. Hallsson

3 Biotech ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Esmaeil Khaleghi ◽  
Karim Sorkheh ◽  
Maryam Hosseni Chaleshtori ◽  
Sezai Ercisli

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