Effect of Sclerotinia sclerotiorum on lipid metabolism in Arabidopsis thaliana

2017 ◽  
Vol 124 (5) ◽  
pp. 421-426 ◽  
Author(s):  
Xiujuan Wang ◽  
Junmin Li ◽  
Peng Zhu
Keyword(s):  
Arabidopsis Thaliana ◽  
Lipid Metabolism ◽  
Sclerotinia Sclerotiorum

scholarly journals Sclerotinia sclerotiorum infection triggers changes in primary and secondary metabolism in Arabidopsis thaliana

2020 ◽  
Author(s):  
Jingyuan Chen ◽  
Chhana Ullah ◽  
Daniel Giddings Vassão ◽  
Michael Reichelt ◽  
Jonathan Gershenzon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Secondary Metabolism ◽  
Sclerotinia Sclerotiorum ◽  
Fungal Pathogens ◽  
Soluble Sugars ◽  
Fungal Colonization ◽  
Plant Responses ◽  
Defense Compounds ◽  
Antifungal Metabolites ◽  
Primary And Secondary Metabolism

Sclerotinia sclerotiorum is a devastating plant pathogen that causes substantial losses in various agricultural crops. Although plants have developed some well-known defense mechanisms against invasive fungi, much remains to be learned about plant responses to fungal pathogens. In this study we investigated how plant primary and secondary metabolism in the model plant Arabidopsis thaliana are affected by S. sclerotiorum infection. Our results showed that the contents of soluble sugars and amino acids changed significantly in A. thaliana leaves upon fungal colonization, with a decrease in sucrose and an increase in mannitol, attributed to fungal biosynthesis. Furthermore, the jasmonate signaling pathway was rapidly activated by S. sclerotiorum infection, and there was a striking accumulation of antifungal metabolites, such as camalexin, p-coumaroyl agmatine, feruloyl agmatine, and Nδ-acetylornithine. On the other hand, the characteristic defense compounds of the Brassicaceae, the glucosinolates, were not induced in A. thaliana infected by the fungus. Our study provides a better understanding of how A. thaliana primary and secondary metabolism are modified during infection by a fungal pathogen like S. sclerotiorum that has both hemibiotrophic and necrotrophic stages.

scholarly journals Effect of Drought Stress on Lipid Metabolism in the Leaves of Arabidopsis thaliana (Ecotype Columbia)

2004 ◽  
Vol 94 (3) ◽  
pp. 345-351 ◽  
Author(s):  
AGNÈS GIGON ◽  
ANA-RITA MATOS ◽  
DANIEL LAFFRAY ◽  
YASMINE ZUILY-FODIL ◽  
ANH-THU PHAM-THI
Keyword(s):  
Arabidopsis Thaliana ◽  
Lipid Metabolism ◽  
Drought Stress

scholarly journals The Geomagnetic Field (GMF) Modulates Nutrient Status and Lipid Metabolism during Arabidopsis thaliana Plant Development

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1729
Author(s):  
Monirul Islam ◽  
Gianpiero Vigani ◽  
Massimo E. Maffei
Keyword(s):  
Magnetic Field ◽  
Arabidopsis Thaliana ◽  
Lipid Metabolism ◽  
Plant Development ◽  
Stress Responses ◽  
Geomagnetic Field ◽  
Time Course ◽  
Seed Set ◽  
Developmental Stages ◽  
Lipid Transfer Protein

The Geomagnetic field (GMF) is a typical component of our planet. Plant perception of the GMF implies that any magnetic field (MF) variation would induce possible metabolic changes. In this work was we assessed the role of the GMF on Arabidopsis thaliana Col0 mineral nutrition and lipid metabolism during plant development. We reduced the local GMF (about 40 μT) to Near Null Magnetic Field (NNMF, about 30 nT) to evaluate the effects of GMF on Arabidopsis in a time-course (from rosette to seed-set) experiment by studying the lipid content (fatty acids, FA; and surface alkanes, SA) and mineral nutrients. The expression of selected genes involved in lipid metabolism was assessed by Real-Time PCR (qPCR). A progressive increase of SA with carbon numbers between 21 and 28 was found in plants exposed to NNMF from bolting to flowering developmental stages, whereas the content of some FA significantly (p < 0.05) increased in rosette, bolting and seed-set developmental stages. Variations in SA composition were correlated to the differential expression of several Arabidopsis 3-ketoacyl-CoAsynthase (KCS) genes, including KCS1, KCS5, KCS6, KCS8, and KCS12, a lipid transfer protein (LTPG1) and a lipase (LIP1). Ionomic analysis showed a significant variation in some micronutrients (Fe, Co, Mn and Ni) and macronutrients (Mg, K and Ca) during plant development of plants exposed to NNMF. The results of this work show that A. thaliana responds to variations of the GMF which are perceived as is typical of abiotic stress responses.

Characterization of an acyl-CoA synthetase from Arabidopsis thaliana

2000 ◽  
Vol 28 (6) ◽  
pp. 957-958 ◽  
Author(s):  
J. A. Schnurr ◽  
J. Shockey ◽  
John Browse
Keyword(s):  
Arabidopsis Thaliana ◽  
Lipid Metabolism ◽  
Reverse Genetics ◽  
Cell Function ◽  
De Novo ◽  
Plant Biotechnology ◽  
Plant Lipid ◽  
Oilseed Crops ◽  
Isolation And Characterization

One of the major goals of modern plant biotechnology is to manipulate lipid metabolism in oilseed crops to produce new and improved edible and industrial vegetable oils. Lipids constitute the structural components of cellular membranes and act as sources of energy for the germinating seed and are therefore essential to plant cell function. Both de novo synthesis and modification of existing lipids are dependent on the activity of acyl-CoA synthetases (ACSs). To date, ACSs have been recalcitrant to traditional methods of purification due to their association with membranes. In our laboratory, several isoforms of ACSs have been identified in Arabidopsis thaliana. Reverse genetics allowed us to identify a mutant containing a transfer DNA-interrupted ACS gene. Results will be presented that describe the isolation and characterization of this mutant. The elucidation of the specific roles of ACSs will lead to a greater understanding of plant lipid metabolism.

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