Characterization of new yeast isolates collected from different fruits in Tunisia and biocontrol activity against Penicillium expansum on apples

2021 ◽  
Author(s):  
Amina Kheireddine ◽  
Davide Palmieri ◽  
Domenico Vitullo ◽  
Antonia Barberio ◽  
Mohamed Zouaoui ◽  
...  
Keyword(s):  
Penicillium Expansum ◽  
Biocontrol Activity ◽  
New Yeast

Characterization of new yeast lipases

1997 ◽  
Vol 74 (11) ◽  
pp. 1391-1394 ◽  
Author(s):  
Ching T. Hou
Keyword(s):  
New Yeast

Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin

2010 ◽  
Vol 56 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Simona Marianna Sanzani ◽  
Leonardo Schena ◽  
Annalisa De Girolamo ◽  
Antonio Ippolito ◽  
Luis González-Candelas
Keyword(s):  
Induced Resistance ◽  
Apple Fruit ◽  
Penicillium Expansum

scholarly journals Recovery of Fusarium oxysporum Fo47 Mutants Affected in Their Biocontrol Activity After Transposition of the Fot1 Element

2002 ◽  
Vol 92 (9) ◽  
pp. 936-945 ◽  
Author(s):  
Sophie Trouvelot ◽  
Chantal Olivain ◽  
Ghislaine Recorbet ◽  
Quirico Migheli ◽  
Claude Alabouvette
Keyword(s):  
Fusarium Oxysporum ◽  
Insertional Mutagenesis ◽  
Wild Type Strain ◽  
Growth Habit ◽  
Antagonistic Activity ◽  
Phenotypic Characterization ◽  
Wild Type ◽  
Biocontrol Activity

To investigate the biocontrol mechanisms by which the antagonistic Fusarium oxysporum strain Fo47 is active against Fusarium wilt, a Fot1 transposon-mediated insertional mutagenesis approach was adopted to generate mutants affected in their antagonistic activity. Ninety strains in which an active Fot1 copy had transposed were identified with a phenotypic assay for excision and tested for their biocontrol activity against F. oxysporum f. sp. lini on flax in greenhouse experiments. Sixteen strains were affected in their capacity to protect flax plants, either positively (more antagonistic than Fo47) or negatively (less antagonistic). The molecular characterization of these mutants confirms the excision of Fot1 and its reinsertion in most of the cases. Moreover, we demonstrate that other transposable elements such as Fot2, impala, and Hop have no transposition activity in the mutant genomes. The phenotypic characterization of these mutants shows that they are affected neither in their in vitro growth habit nor in their competitiveness in soil compared with wild-type strain Fo47. These results show that mutants are not impaired in their saprophytic phase and suggest that the altered biocontrol phenotype should likely be expressed during the interaction with the host plant.

scholarly journals Identification and characterization of LysM effectors in Penicillium expansum

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0186023 ◽  
Author(s):  
Elena Levin ◽  
Ana Rosa Ballester ◽  
Ginat Raphael ◽  
Oleg Feigenberg ◽  
Yongsheng Liu ◽  
...  
Keyword(s):  
Penicillium Expansum ◽  
Identification And Characterization

scholarly journals Characterization of the Exoglucanase-Encoding Gene PaEXG2 and Study of Its Role in the Biocontrol Activity of Pichia anomala Strain K

2003 ◽  
Vol 93 (9) ◽  
pp. 1145-1152 ◽  
Author(s):  
Cathy Grevesse ◽  
Philippe Lepoivre ◽  
Mohamed Haïssam Jijakli
Keyword(s):  
Marker Gene ◽  
Glycosylation Site ◽  
Pichia Anomala ◽  
Gene Encoding ◽  
Biocontrol Activity ◽  
Monophosphate Decarboxylase ◽  
Uracil Auxotroph

The PaEXG2 gene, encoding an exo-β-1,3-glucanase, was isolated from the biocontrol agent Pichia anomala strain K. PaEXG2 has the capacity for coding an acidic protein of 427 amino acids with a predicted molecular weight of 45.7 kDa, a calculated pI of 4.7, and one potential N-glycosylation site. PaEXG2 was disrupted by the insertion of the URA3 marker gene, encoding orotidine monophosphate decarboxylase in strain KU1, a uracil auxotroph derived from strain K. Strain KU1 showed inferior biocontrol activity and colonization of wounds on apples, compared to the prototrophic strain. Antagonism and colonization were recovered after the restoration of prototrophy by transformation with the URA3 gene. Integrative transformation was shown to be mostly ectopic in strain K descendants (only 4% of integration by homologous recombination). PaEXG2 disruption abolished all detectable extracellular exo-β-1,3-glucanase activity in vitro and in situ but did not affect biocontrol of Botrytis cinerea on wounded apples.

scholarly journals Characterization of Penicillium Isolates Associated with Blue Mold on Apple in Uruguay

Plant Disease ◽  
2004 ◽  
Vol 88 (1) ◽  
pp. 23-28 ◽  
Author(s):  
M. J. Pianzzola ◽  
M. Moscatelli ◽  
S. Vero
Keyword(s):  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Species Level ◽  
Penicillium Expansum ◽  
Postharvest Disease ◽  
Pear Fruit ◽  
Blue Mold ◽  
Penicillium Spp ◽  
Its1 And Its2

Blue mold caused by Penicillium spp. is the most important postharvest disease of apple in Uruguay. Fourteen isolates of Penicillium were recovered from rotten apple and pear fruit with blue mold symptoms, and from water from flotation tanks in commercial apple juice facilities. Phenotypic identification to species level was performed, and the isolates were tested for sensitivity to commonly used postharvest fungicides. Genetic characterization of the isolates was performed with restriction fragment length polymorphism of the region including the internal transcribed spacer (ITS) ITS1 and ITS2 and the 5.8SrRNA gene (ITS1-5.8SrRNA gene-ITS2) ribosomal DNA region and with random amplified polymorphic DNA (RAPD) primers. Both techniques were able to differentiate these isolates at the species level. RAPD analysis proved to be an objective, rapid, and reliable tool to identify Penicillium spp. involved in blue mold of apple. In all, 11 isolates were identified as Penicillium expansum and 3 as P. solitum. This is the first report of P. solitum as an apple pathogen in Uruguay.

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