scholarly journals Improve gamma-aminobutyric acid production in Corynebacterium glutamicum by optimizing the metabolic flux

2021 ◽  
Author(s):  
Chengzhen Yao ◽  
Yuantao Liu ◽  
Xiaoqing Hu ◽  
Xiaoyuan Wang
Keyword(s):  
Corynebacterium Glutamicum ◽  
Acid Production ◽  
Metabolic Flux ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid

Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli

2015 ◽  
Vol 178 (7) ◽  
pp. 1315-1324 ◽  
Author(s):  
Van Dung Pham ◽  
Sivachandiran Somasundaram ◽  
Seung Hwan Lee ◽  
Si Jae Park ◽  
Soon Ho Hong
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Metabolic Flux ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Synthetic Scaffolds

scholarly journals Construction of a Vitreoscilla Hemoglobin Promoter-Based Tunable Expression System for Corynebacterium glutamicum

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 561 ◽  
Author(s):  
Kei-Anne Baritugo ◽  
Hee Taek Kim ◽  
Mi Na Rhie ◽  
Seo Young Jo ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Fluorescent Protein ◽  
Expression System ◽  
Vitreoscilla Hemoglobin ◽  
Aminobutyric Acid ◽  
Vector System ◽  
Efficient Production ◽  
Gamma Aminobutyric Acid ◽  
Recombinant Strains ◽  
Gaba Production

Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (Pvgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of Pvgb (Pvgb, Pvgb4, and Pvgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of Pvgb increased from single to eight (Pvgb8) repeats. Furthermore, we demonstrated the application of the new Pvgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadBmut. We observed that 5-AVA and GABA production by the recombinant strains increased as the number of Pvgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of Pvgb8 (3.69 ± 0.07 g/L) than the one under the control of Pvgb (3.43 ± 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of Pvgb used for GadBmut expression increased from single (4.81–5.31 g/L) to eight repeats (4.94–5.58 g/L).

Comparison of gamma-aminobutyric acid production in Thai rice grains

2009 ◽  
Vol 26 (2) ◽  
pp. 257-263 ◽  
Author(s):  
Panatda Jannoey ◽  
Hataichanoke Niamsup ◽  
Saisamorn Lumyong ◽  
Toshisada Suzuki ◽  
Takeshi Katayama ◽  
...  
Keyword(s):  
Acid Production ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Rice Grains

A new metabolic route for the production of gamma-aminobutyric acid by Corynebacterium glutamicum from glucose

Amino Acids ◽  
2016 ◽  
Vol 48 (11) ◽  
pp. 2519-2531 ◽  
Author(s):  
João M. P. Jorge ◽  
Christian Leggewie ◽  
Volker F. Wendisch
Keyword(s):  
Corynebacterium Glutamicum ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Metabolic Route
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