Effects of Application of Pig Manure on the Accumulation of Heavy Metals in Rice

Pig manure (PM) is often highly enriched in heavy metals, such as Cu and Zn, due to the wide use of feed additives. To study the potential risks of heavy metal accumulation in the soil and rice grains by the application of PM and other organic manure, a four-year field experiment was conducted in the suburb of Shanghai, southeast China. The contents of Cu, Zn, Pb, and Cd in the soils and rice plants by the treatments of PM and fungal culturing residues (FCR) show a trend of annual increase. Those in the soils and rice by the PM treatment are raised even more significantly. Cu and Zn contents in the soil and rice roots by the PM are significantly higher than those by the non-fertilizer control (CK) during the four years, and Pb and Cd also significantly higher than CK in the latter two years. Heavy metals taken up by the rice plants are mostly retained in the roots. Cu and Zn contents in the rice plants are in the decreasing order of roots > grains > stems > leaves, and Pb and Cd in the order of roots > stems > leaves > grains. Cu, Zn, Pb, and Cd contents in the soils by the PM treatment increase by 73%, 32%, 106%, and 127% on annual average, and those in the brown rice by 104%, 98%, 275%, and 199%, respectively. The contents of Cu, Zn, Pb, and Cd in the brown rice of the treatments are significantly correlated with those in the soils and rice roots (p < 0.05), suggesting the heavy metals accumulated in the rice grains come from the application of PM and FCR. Though the contents of heavy metals in the brown rice during the four experimental years are still within the safe levels, the risks of their accumulative increments, especially by long-term application of PM, can never be neglected.

Discrete element method investigation of the milling characteristic in a rice mill

A milling chamber consisting of a rice sieve and a rotating roller plays critical roles in modulating the milling performance of rice grains. However, the mechanism of how the geometries of the rice sieve and rotating roller affect the particle collisions and the interaction time remains not fully understood. Our experimental results show that the milling degree and rate of broken rice of the octagonal rice sieve are largest among the hexagonal sieve, octagonal sieve, and circular sieve. Through the discrete element method, we illustrate that the peak milling degree at the octagonal sieve is attributed to the competition between the decreasing force and increasing milling time with the increase in edges. In addition, the geometries of the convex ribs of the rotating roller are investigated to optimize the structure of the milling chamber. In the left-hand spiral or right-hand spiral of the convex ribs, the rice particles are accumulated in the inlet or outlet regions, respectively, which leads to an uneven milling degree in the axial direction. The uniformity of a milling process can be promoted by increasing the number of convex ribs, which will reduce the milling degree on the other hand.

The Vacuolar Transporter OsNRAMP2 Mediates Fe Remobilization During Germination and Affects Cd Distribution to Rice Grain

Background and aims Iron (Fe) deficiency in plants is a common problem affecting agricultural production. Cadmium (Cd) is a toxic metal that can be taken up and transported within plants by transporters for divalent metals including Fe(II). The present study aims to investigate the functions of OsNRAMP2 (Natural Resistance-Associated Macrophage Protein 2) in the remobilization and distribution of Fe and Cd in rice. Methods The expression pattern of OsNRAMP2 was determined by quantitative real-time PCR and pOsNRAMP2:GUS assay. Knockout mutants of OsNRAMP2 were generated by using CRISPR/Cas9 gene editing. Localization of Fe in the vacuolar globoids of germinating seeds was imaged by high-resolution transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy. Distributions of Fe and Cd between different plant tissues were investigated in hydroponic and soil pot experiments. Results OsNRAMP2 was mainly expressed in the embryo of germinating seeds, roots, leaf sheaths and leaf blades. OsNRAMP2 was localized at the tonoplast. Knockout of OsNRAMP2 delayed seed germination and produced chlorotic seedling leaves. Remobilization of Fe stored in the protein storage vacuoles in the scutellum of germinating seeds was restricted in osnramp2 mutants compared with wild type. Expression of genes related to Fe uptake was enhanced in the seedlings of osnramp2 mutants. Knockout of OsNRAMP2 significantly decreased the distribution of Cd, but not Fe, from leaves and straws to rice grains. Conclusions OsNRAMP2 plays an important role in remobilizing vacuolar Fe during seed germination and affects translocation of Cd from vegetative tissues to rice grains.

Chemical and sensory properties of analogue rice based on kimpul flour (Xanthosoma sagitifolium)

Analog rice is artificial rice shaped like rice grains made from non-rice carbohydrate-rich flour with water, which can overcome food security in Indonesia. Taro kimpul is a local food rich in carbohydrates that cannot be widely used. Therefore, kimpul thread has the potential to be used as raw material in the manufacture of analog rice. This study aimed to determine the chemical characteristics of kimpul taro analog rice with dyes and binders. In addition, it is expected to increase consumer acceptance based on sensory testing. This research method uses an experimental laboratory method by making analog rice with 4 formulations. The analysis was water content, ash content, protein content, fat content, carbohydrate content, and sensory (hedonic) analysis, including colour, taste, texture, and overall aroma. The results showed that analog rice A was the best formula selected using the Bayes method based on the results of chemical and hedonic tests. Chemical and sensory characteristics of analog rice A with the use of 4% CMC and 32% beet are as follows moisture 2.88%; ash 2.3%; fat 1.1%; 5.7% protein; carbohydrate 87.94% and a preference value with an average range of neutral-good.