Neuropathic Pain Model, Spinal Nerve Ligation Model

2012 ◽  
Keyword(s):  
Neuropathic Pain ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Pain Model ◽  
Neuropathic Pain Model

scholarly journals miRNA 146a-5p-loaded poly(d,l-lactic-co-glycolic acid) nanoparticles impair pain behaviors by inhibiting multiple inflammatory pathways in microglia

Nanomedicine ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. 1113-1126
Author(s):  
Thuỳ Linh Phạm ◽  
Yuhua Yin ◽  
Hyeok Hee Kwon ◽  
Nara Shin ◽  
Song I Kim ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Inflammatory Response ◽  
P38 Mapk ◽  
Glycolic Acid ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Pain Model ◽  
Pain Behaviors ◽  
Analgesic Effects ◽  
Neuropathic Pain Model

Aims: We investigated whether miRNA (miR) 146a-5p-loaded nanoparticles (NPs) can attenuate neuropathic pain behaviors in the rat spinal nerve ligation-induced neuropathic pain model by inhibiting activation of the NF-κB and p38 MAPK pathways in spinal microglia. Materials & methods: After NP preparation, miR NPs were assessed for their physical characteristics and then injected intrathecally into the spinal cords of rat spinal nerve ligation rats to test their analgesic effects. Results: miR NPs reduced pain behaviors for 11 days by negatively regulating the inflammatory response in spinal microglia. Conclusion: The anti-inflammatory effects of miR 146a-5p along with nanoparticle-based materials make miR NPs promising tools for treating neuropathic pain.

scholarly journals A Comparison of Surgical Invasions for Spinal Nerve Ligation with or without Paraspinal Muscle Removal in a Rat Neuropathic Pain Model

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yi-Gang Huang ◽  
Qing Zhang ◽  
Hao Wu ◽  
Chang-Qing Zhang
Keyword(s):  
Neuropathic Pain ◽  
Interleukin 1 ◽  
Physiological Model ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Paraspinal Muscle ◽  
Surgical Trauma ◽  
Physiological Processes ◽  
Neuropathic Pain Model ◽  
Removal Group

L5 spinal nerve ligation (SNL) in rats is one of the most popular models for studying neuropathic pain because of its high reproducibility. During the surgery, a part of the L5 paraspinal muscle is usually removed, which produces extra trauma and may potentially affect the physiological processes involved in neuropathic pain. To reduce the surgical trauma, the paraspinal muscle retraction was developed for exposure of the spinal nerve. The current study was aimed at comparing the surgical invasions between the L5 SNL models with paraspinal muscle removal or retraction. The results showed that both methods induced similar neuropathic pain behavior. However, the paraspinal muscle retraction group exhibited an average of 2.7 mg less blood loss than the muscle removal group. This group also showed a significantly lower increase in serum myoglobin and creatine phosphokinase levels on postoperative days 1 and 2, as well as a lower increase in interleukin-1βand interleukin-6 levels on postoperative day 1. The paraspinal muscle maintained normal morphological features following paraspinal muscle retraction. Our results indicate that the SNL rat model with paraspinal muscle retraction is a reliable physiological model that is reproducible, readily available, and less invasive than the model with muscle removal.

scholarly journals Effect of Methylcobalamin on Voltage-Gated Sodium Channels (Vgscs ) Expression in Neuropathic Painanimal Model

2021 ◽  
Vol 14 (02) ◽  
pp. 1033-1038
Author(s):  
Endang Mutiawati ◽  
K.R.T. Lucas Meliala ◽  
Ginus Partadiredja ◽  
Dhirgo Adji ◽  
Raden Wasito
Keyword(s):  
Neuropathic Pain ◽  
Sodium Channels ◽  
Mechanical Allodynia ◽  
Spinal Nerve ◽  
Control Group ◽  
Pain Model ◽  
Voltage Gated ◽  
Voltage Gated Sodium Channels ◽  
Neuropathic Pain Model ◽  
First Onset

The objective of this study wasto assess the effect of methylcobalaminonmechanical allodynia and the voltage-gated sodium channels (VGSCs) expression of injured nerves in spinal nerve ligation-induced neuropathic pain model in animals.Three different doses of methylcobalamin were administrated intramuscularly into neuropathic pain rat model, twice a week for 14 weeks. The effect of methylcobalamin on neuropathic pain was assessed using mechanical allodynia (using the von Frey filaments) while its effect on VGSC expression was assessed using immunohistochemistry. ANOVA and independent t-test were employed to compared the effect of methylcobalamin on mechanical allodynia between groups.The size of von Frey filament that induced the first onset of mechanical allodynia was smaller in control group compared to 50µg methylcobalamin group (p=0.013) and methylcobalamin 100µg group (p=0.019). There is a dose–response relationship between methylcobalamin dose and the average duration of mechanical allodynia (43.8, 38.2, 30.6 and 29.6 days for control, 50µg, 100µg, and 150µg methylcobalamin group, respectively) with a significant different observed between control and 150µg methylcobalamin group only (p=0.027). Nerve tissues from all animals within control group expressed VGSC while all nerve tissues from both 100µg, and 150µg methylcobalamin, had no VGCS expression. In conclusion, methylcobalamin is potentially shorten the duration of mechanical allodynia and increase pain threshold in neuropathic pain animal model. These effects might associate with reduction of VGSC expression on the injured neurons.

scholarly journals Dexmedetomidine versus ketamine in attenuating neuropathic pain through modulating STING/TBK pathway to modulate spinal ER-phagy in neuropathic pain model

2020 ◽  
Author(s):  
Yongda Liu ◽  
Mengmeng Ding ◽  
Zhibin Wang ◽  
Xingyue Li ◽  
Jiao Guo ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Er Stress ◽  
Cellular Localization ◽  
Open Field Test ◽  
Spinal Nerve ◽  
Pain Model ◽  
Withdrawal Threshold ◽  
Confocal Immunofluorescence ◽  
Neuropathic Pain Model ◽  
Sting Pathway

Abstract Background: Our previous studies suggested that stimulator of interferon genes (STING) level was altered in medial prefrontal cortex (mPFC) of spinal nerve ligation (SNL) rats.Methods: In this study, we investigated that whether dexmedetomidine and ketamine provide antianxiety and anti-nociceptive effects via modulating spinal STING/TBK pathway to alter ER-phagy in SNL rats. We evaluated the analgesia and antianxiety effects of ketamine and dexmedetomidine in SNL rats. 2’3’-cGAMP (STING pathway agonist) was administrated to investigate whether enhanced spinal STING pathway could reverse dexmedetomidine or ketamine treatment effects in SNL rats respectively. Analgesia effects were measured with mechanical withdrawal threshold (MWT) and antianxiety effect was measured with open field test (OFT). Proteins expression levels were evaluated by Western blotting. Distribution and cellular localization of STING pathway and ER stress were evaluated by confocal immunofluorescence.Results: SNL induced mechanical hypersensitivity and anxiety; STING pathway was involved in the modulation of ER stress and ER-phagy in SNL rats; Dexmedetomidine and ketamine provided analgesia and antianxiety effects in SNL rats; Dexmedetomidine and ketamine alleviated ER stress via inhibiting STING pathway to enhance ER-phagy in SNL rats.Conclusions: In all, both ketamine and dexmedetomidine provided antianxiety and anti-nociceptive effects through alleviating ER stress via inhibiting STING/TBK pathway to modulate spinal ER-phagy in SNL rats.

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