Determination of carbohydrate heterogeneity in the humanised antibody CAMPATH 1H by liquid chromatography and matrix-assisted laser desorption ionisation mass spectrometry

1995 ◽  
Vol 306 (1) ◽  
pp. 43-48 ◽  
Author(s):  
David S. Ashton ◽  
Christopher R. Beddell ◽  
David J. Cooper ◽  
Anne C. Lines
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Laser Desorption ◽  
Ionisation Mass Spectrometry ◽  
Ionisation Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Preliminary Silylation for Structure Determination of Oligomeric Silsesquioxanes by Matrix-Assisted Laser Desorption/Ionisation Mass Spectrometry

2009 ◽  
Vol 15 (2) ◽  
pp. 231-238 ◽  
Author(s):  
V.G. Zaikin ◽  
R.S. Borisov ◽  
N.Yu. Polovkov ◽  
S.N. Filatov ◽  
V.V. Kireev
Keyword(s):  
Mass Spectrometry ◽  
Structure Elucidation ◽  
Laser Desorption ◽  
Hydroxyl Groups ◽  
Ionisation Mass Spectrometry ◽  
First Time ◽  
Ionisation Mass ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The potential of preliminary silylation for the determination of numbers of hydroxyl groups in separate molecules of oligomeric silsesquioxanes by matrix-assisted laser desorption/ionisation mass spectrometry has been demonstrated for the first time. Derivatisations allowing the introduction of trimethylsilyl, tert-butyldimethylsilyl and pentafluorophenyldimethylsilyl groups were tested. It was shown that more reliable structure elucidation of individual oligomers can be achieved knowing the number of hydroxyl groups thus determined.

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