Analysis of the free nucleotide pools of mammalian tissues by high-pressure liquid chromatography

1982 ◽  
Vol 123 (2) ◽  
pp. 378-388 ◽  
Author(s):  
Eric G. Brown ◽  
Russell P. Newton ◽  
Nicholas M. Shaw
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Nucleotide Pools ◽  
Free Nucleotide ◽  
Mammalian Tissues

scholarly journals Nucleotide Profiles of the Formed Elements of Human Blood Determined by High-Pressure Liquid Chromatography

Blood ◽  
1973 ◽  
Vol 41 (6) ◽  
pp. 927-936 ◽  
Author(s):  
E. M. Scholar ◽  
Phyllis R. Brown ◽  
R. E. Parks ◽  
P. Calabresi
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Human Blood ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Guanine Nucleotide ◽  
Disease States ◽  
Nucleotide Pools ◽  
Unique Potential

Abstract The nucleotide patterns of the acid soluble extracts of the formed elements of human peripheral blood were determined by high-pressure liquid chromatography. The patterns are remarkably reproducible for each of the respective elements, and the concentration of each major nucleotide present was determined. Erythrocytes are characterized by relatively simple nucleotide patterns with high adenine and very low guanine concentrations. The chromatograms of granulocytes and lymphocytes are difficult to distinguish from one another and demonstrate substantially greater UTP and guanine nucleotide levels than are observed in erythrocytes. The nucleotide patterns of platelets revealed a significantly lower ATP/ADP ratio than did either leukocytes or erythrocytes. The unique potential of this new technique for monitoring changes in nucleotide pools in normal and disease states is discussed.

Historical chemical annotations of Cinchona bark collections are comparable to results from current day high-pressure liquid chromatography technologies

2020 ◽  
Vol 249 ◽  
pp. 112375 ◽  
Author(s):  
Nataly Allasi Canales ◽  
Tobias Nikolaj Gress Hansen ◽  
Claus Cornett ◽  
Kim Walker ◽  
Felix Driver ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography
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