Molecular cloning of complementary DNA: Preparation of a plasmid vector with low transformation background

1983 ◽  
Vol 129 (1) ◽  
pp. 249-252 ◽  
Author(s):  
Anne Leriche ◽  
Daniel Christophe ◽  
Huguette Brocas ◽  
Gilbert Vassart
Keyword(s):  
Molecular Cloning ◽  
Plasmid Vector ◽  
Complementary Dna ◽  
Dna Preparation

Molecular Cloning and Nucleotide Sequence of Complementary DNA for Human Hepatic Cytosolic Acetoacetyl-Coenzyme A Thiolase

1994 ◽  
Vol 201 (1) ◽  
pp. 478-485 ◽  
Author(s):  
X.Q. Song ◽  
T. Fukao ◽  
S. Yamaguchi ◽  
S. Miyazawa ◽  
T. Hashimoto ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Cloning ◽  
Coenzyme A ◽  
Complementary Dna

Molecular cloning of the complementary DNA copies of the common and cowpea strains of tobacco mosaic virus RNA

Virology ◽  
1982 ◽  
Vol 118 (1) ◽  
pp. 64-75 ◽  
Author(s):  
Tetsuo Meshi ◽  
Nobuhiko Takamatsu ◽  
Takeshi Ohno ◽  
Yoshimi Okada
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Complementary Dna ◽  
Virus Rna ◽  
The Common

scholarly journals Mega primer-mediated molecular cloning strategy for chimaeragenesis and long DNA fragment insertion

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Hui Zhang ◽  
Chang-Jun Liu ◽  
Hui Jiang ◽  
Lu Zhou ◽  
Wen-Ying Li ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
High Efficiency ◽  
Low Cost ◽  
Plasmid Vector ◽  
Efficient Tool ◽  
Overlap Extension ◽  
Cloning Strategy ◽  
Dna Fragment ◽  
Fragment Insertion ◽  
Dpni Digestion

Molecular cloning methods based on primer and overlap-extension PCR are widely used due to their simplicity, reliability, low cost and high efficiency. In this article, an efficient mega primer-mediated (MP) cloning strategy for chimaeragenesis and long DNA fragment insertion is presented. MP cloning is a seamless, restriction/ligation-independent method that requires only three steps: (i) the first PCR for mega primer generation; (ii) the second PCR for exponential amplification mediated by the mega primers and (iii) DpnI digestion and transformation. Most importantly, for chimaeragenesis, genes can be assembled and constructed into the plasmid vector in a single PCR step. By employing this strategy, we successfully inserted four DNA fragments (approximately 500 bp each) into the same vector simultaneously. In conclusion, the strategy proved to be a simple and efficient tool for seamless cloning.

Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system

Science ◽  
1979 ◽  
Vol 203 (4383) ◽  
pp. 883-887 ◽  
Author(s):  
M. Israel ◽  
H. Chan ◽  
W. Rowe ◽  
M. Martin
Keyword(s):  
Escherichia Coli ◽  
Molecular Cloning ◽  
Plasmid Vector ◽  
Polyoma Virus ◽  
Vector System ◽  
Coli Plasmid
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