Determination of Sepharose-bound protein with Coomassie brilliant blue G-250

1985 ◽  
Vol 151 (2) ◽  
pp. 571-574 ◽  
Author(s):  
R.A. Asryants ◽  
I.V. Duszenkova ◽  
N.K. Nagradova
1989 ◽  
Vol 35 (10) ◽  
pp. 2127-2129 ◽  
Author(s):  
Y Bergqvist ◽  
L Karlsson ◽  
L Fohlin

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.


1981 ◽  
Vol 113 (1) ◽  
pp. 197-201 ◽  
Author(s):  
Kiyoko Sano ◽  
Kiyoko Kanamori ◽  
Akihiko Shiba ◽  
Makoto Nakao

Sign in / Sign up

Export Citation Format

Share Document