Coomassie brilliant blue G-250 dye-binding technique for determination of autolytic protein breakdown in Euglena gracilis and comparison to other methods of autolysis measurement

1986 ◽  
Vol 153 (2) ◽  
pp. 242-250 ◽  
Author(s):  
Rainald Krauspe ◽  
Angelika Scheer
Keyword(s):  
Euglena Gracilis ◽  
Brilliant Blue ◽  
Protein Breakdown ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Dye Binding ◽  
Coomassie Brilliant

Determination of Sepharose-bound protein with Coomassie brilliant blue G-250

1985 ◽  
Vol 151 (2) ◽  
pp. 571-574 ◽  
Author(s):  
R.A. Asryants ◽  
I.V. Duszenkova ◽  
N.K. Nagradova
Keyword(s):  
Brilliant Blue ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant

Comparison of Coomassie Brilliant Blue protein dye-binding assays for determination of urinary protein concentration.

1987 ◽  
Vol 33 (4) ◽  
pp. 577-578 ◽  
Author(s):  
T Marshall ◽  
K M Williams
Keyword(s):  
Protein Concentration ◽  
Urinary Protein ◽  
Brilliant Blue ◽  
Binding Assays ◽  
Coomassie Brilliant Blue ◽  
Standard Assay ◽  
Dye Binding ◽  
Coomassie Brilliant ◽  
Assay Protocol

Abstract We investigated the influence of assay protocol upon results of Coomassie Brilliant Blue protein dye-binding assays for urinary protein. Sensitivity and working range for, and linearity of response (including dilution dependence) of, three standard assays and four micro assays to bovine albumin, gamma-globulin, and urine are compared. The Read and Northcote standard assay (Anal Biochem 1981; 116:53-64) appears to be the method of choice.

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

1989 ◽  
Vol 35 (10) ◽  
pp. 2127-2129 ◽  
Author(s):  
Y Bergqvist ◽  
L Karlsson ◽  
L Fohlin
Keyword(s):  
Breast Milk ◽  
Total Protein ◽  
Brilliant Blue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Simple Method ◽  
Coomassie Brilliant Blue ◽  
Kjeldahl Method ◽  
Coomassie Brilliant

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

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