Aromatic hydroxylation of phenylalanine as an assay for hydroxyl radicals: Application to activated human neutrophils and to the heme protein leghemoglobin

1988 ◽  
Vol 172 (2) ◽  
pp. 360-367 ◽  
Author(s):  
Harparkash Kaur ◽  
Ingrid Fagerheim ◽  
Martin Grootveld ◽  
Alain Puppo ◽  
Barry Halliwell
Keyword(s):  
Hydroxyl Radicals ◽  
Heme Protein ◽  
Human Neutrophils ◽  
Aromatic Hydroxylation

EPR studies on the generation of superoxide and hydroxyl radicals by human neutrophils

1995 ◽  
Vol 45 (6) ◽  
pp. 974
Author(s):  
H. Struy ◽  
D. Schmidt ◽  
R. Gärtner ◽  
J. Morenz
Keyword(s):  
Hydroxyl Radicals ◽  
Human Neutrophils

Aromatic hydroxylation in PBN spin trapping by hydroxyl radicals and cytochrome P-450

2000 ◽  
Vol 28 (3) ◽  
pp. 345-350 ◽  
Author(s):  
Lester A Reinke ◽  
Danny R Moore ◽  
Hong Sang ◽  
Edward G Janzen ◽  
Yashige Kotake
Keyword(s):  
Hydroxyl Radicals ◽  
Spin Trapping ◽  
Aromatic Hydroxylation ◽  
Cytochrome P 450

Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation

1988 ◽  
Vol 46 ◽  
pp. 138-139
Author(s):  
J. Hanker ◽  
B. Giammara ◽  
G. Strauss
Keyword(s):  
Uv Radiation ◽  
Myeloid Leukemia ◽  
Stratospheric Ozone ◽  
Psoriasis Patient ◽  
Whole Body ◽  
Human Neutrophils ◽  
Cupric Nitrate ◽  
The Earth ◽  
Routine Handling ◽  
Uvb Phototherapy

Only a fraction of the UV radiation emitted by the sun reaches the earth; most of the UVB (290-320nm) is eliminated by stratospheric ozone. There is increasing concern, however, that man-made chemicals are damaging this ozone layer. Although the effects of UV on DNA or as a carcinogen are widely known, preleukemia and acute myeloid leukemia (AML) have only rarely been reported in psoriasis patients treated with 8-methoxypsoralen and UV (PUVA). It was therefore of interest to study the effects of UV on the myeloperoxidase (MP) activity of human neutrophils. The peroxidase activity of enriched leukocyte preparations on coverslips was shown cytochemically with a diaminobenzidine medium and cupric nitrate intensification.Control samples (Figs. 1,4,5) of human bloods that were not specifically exposed to UV radiation or light except during routine handling were compared with samples which had been exposed in one of several different ways. One preparation (Fig. 2) was from a psoriasis patient who had received whole-body UVB phototherapy repeatedly.

Application of high-resolution field-emission LVSEM, backscatter imaging, immunogold staining to definition of the spatial distributions of two human neutrophil adherence receptors

1993 ◽  
Vol 51 ◽  
pp. 36-37
Author(s):  
Robert D. Nelson ◽  
Sharon R. Hasslen ◽  
Stanley L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Three Dimensional ◽  
Human Neutrophils ◽  
Spatial Distributions ◽  
Immunogold Staining ◽  
Functional Control ◽  
Neutrophil Adherence ◽  
Definition Of ◽  
Mode Of Attachment

Receptors are commonly defined in terms of number per cell, affinity for ligand, chemical structure, mode of attachment to the cell surface, and mechanism of signal transduction. We propose to show that knowledge of spatial distribution of receptors on the cell surface can provide additional clues to their function and components of functional control.L-selectin and Mac-1 denote two receptor populations on the neutrophil surface that mediate neutrophil-endothelial cell adherence interactions and provide for targeting of neutrophil recruitment to sites of inflammation. We have studied the spatial distributions of these receptors using LVSEM and backscatter imaging of isolated human neutrophils stained with mouse anti-receptor (primary) antibody and goat anti-mouse (secondary) antibody conjugated to 12 nm colloidal gold. This combination of techniques provides for three-dimensional analysis of the expression of these receptors on different surface membrane domains of the neutrophil: the ruffles and microvilli that project from the cell surface, and the cell body between these projecting structures.

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