Time-resolved immunofluorometric assays with measurement of a Europium chelate in solution: Application for sensitive determination of fibronectin

1991 ◽  
Vol 197 (1) ◽  
pp. 258-265 ◽  
Author(s):  
J. Kropf ◽  
E. Quitte ◽  
A.M. Gressner
Keyword(s):  
Time Resolved ◽  
Sensitive Determination ◽  
Europium Chelate

Determination of Fetal Hemoglobin by Time-Resolved Immunofluorometric Assay

1992 ◽  
Vol 38 (10) ◽  
pp. 2013-2018 ◽  
Author(s):  
U Turpeinen ◽  
U H Stenman
Keyword(s):  
High Performance ◽  
Fetal Hemoglobin ◽  
Gamma Chain ◽  
Time Resolved ◽  
Assay Procedure ◽  
Analytical Recovery ◽  
Sandwich Type ◽  
Capture Antibody ◽  
Europium Chelate

Abstract We have developed a "sandwich"-type time-resolved immunofluorometric assay (IFMA) for fetal hemoglobin (HbF) in hemolysates from adults and newborns, amniotic fluid, and plasma, based on a polyclonal and a monoclonal antibody against human fetal hemoglobin. Microtiter wells are coated with polyclonal capture antibody, and the gamma-chain-specific monoclonal tracer antibody is labeled with a europium chelate. In a simple and fast assay procedure, prediluted hemolysates are incubated in the microtiter wells first with capture antibody for 1 h and, after washing, for 1 h with tracer antibody. The wells are washed and the fluorescence of europium is measured. The mean analytical recovery is 102% and results by IFMA agreed well with values obtained by high-performance liquid chromatography. The analytical range of IFMA is large and well suited for clinical purposes. The detection limit of the assay is 0.2 microgram/L and the measuring range extends to 500 micrograms/L.

Homogeneous time-resolved fluoroimmunoassay of thyroxin in serum.

1988 ◽  
Vol 34 (11) ◽  
pp. 2320-2322 ◽  
Author(s):  
I Hemmilä ◽  
O Malminen ◽  
H Mikola ◽  
T Lövgren
Keyword(s):  
Time Resolved ◽  
Fast Measurement ◽  
Europium Chelate

Abstract We describe a rapid, simple nonseparation fluoroimmunoassay for determination of thyroxin in serum. The assay is based on the labeling of thyroxin directly with a fluorescent europium chelate, the fluorescence of which is quenched on binding to an antithyroxin antibody. With the assay buffer we used, maximum quenching is 90%. The rapid achievement of equilibrium in the assay solution, regardless of the sequence of reagent additions, allows fast measurement of thyroxin. Precision was good (CV less than 5%) within the clinical range for total thyroxin (50-300 nmol/L), and results correlated well with those by a commercial radioimmunoassay.

Sensitive Determination of Fluoroquinolone Antibiotics in Milk Samples Using Time‐Resolved Methodology

2004 ◽  
Vol 37 (6) ◽  
pp. 1163-1175 ◽  
Author(s):  
R. C. Rodríguez‐Díaz ◽  
M. P. Aguilar‐Caballos ◽  
A. Gómez‐Hens
Keyword(s):  
Time Resolved ◽  
Milk Samples ◽  
Fluoroquinolone Antibiotics ◽  
Sensitive Determination

Sample-pretreatment-free based high sensitive determination of aflatoxin M1in raw milk using a time-resolved fluorescent competitive immunochromatographic assay

RSC Advances ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 558-564 ◽  
Author(s):  
Xiaoqian Tang ◽  
Zhaowei Zhang ◽  
Peiwu Li ◽  
Qi Zhang ◽  
Jun Jiang ◽  
...  
Keyword(s):  
Sample Pretreatment ◽  
Raw Milk ◽  
Immunochromatographic Assay ◽  
Time Resolved ◽  
Milk Samples ◽  
Highly Sensitive ◽  
Sensitive Determination

A highly-sensitive TRFICA was developed to detect AFM1in raw milk samples within 6 minutes without any sample pretreatments.

A novel bifunctional europium chelate applied in quantitative determination of human immunoglobin G using time-resolved fluoroimmunoassay

2011 ◽  
Vol 409 (2) ◽  
pp. 244-248 ◽  
Author(s):  
Cheng-Gang Niu ◽  
Jing Liu ◽  
Pin-Zhu Qin ◽  
Guang-Ming Zeng ◽  
Min Ruan ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Time Resolved ◽  
Europium Chelate ◽  
Immunoglobin G
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