A role of catalase in S-methyl group oxidation

Methyltransferase like-3 (METTL3) and METTL14 complex transfers a methyl group from S-adenosyl-L-methionine to N6 amino group of adenosine bases in RNA (m6A) and DNA (m6dA). Emerging evidence highlights a role of METTL3-METTL14 in the chromatin context, especially in processes where DNA and RNA are held in close proximity. However, a mechanistic framework about specificity for substrate RNA/DNA and their interrelationship remain unclear. By systematically studying methylation activity and binding affinity to a number of DNA and RNA oligos with different propensities to form inter- or intra-molecular duplexes or single-stranded molecules in vitro, we uncover an inverse relationship for substrate binding and methylation and show that METTL3-METTL14 preferentially catalyzes the formation of m6dA in single-stranded DNA (ssDNA), despite weaker binding affinity to DNA. In contrast, it binds structured RNAs with high affinity, but methylates the target adenosine in RNA (m6A) much less efficiently than it does in ssDNA. We also show that METTL3-METTL14-mediated methylation of DNA is largely restricted by structured RNA elements prevalent in long noncoding and other cellular RNAs.

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Evidence for metabolism of o-xylene by simultaneous ring and methyl group oxidation in a new soil isolate

Microbiology ◽

10.1099/00221287-143-7-2321 ◽

1997 ◽

Vol 143 (7) ◽

pp. 2321-2329 ◽

Cited By ~ 25

Author(s):

S. R. Bickerdike ◽

R. A. Holt ◽

G. M. Stephens

Keyword(s):

Methyl Group ◽

Soil Isolate ◽

Methyl Group Oxidation

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GLYCINE METHYLTRANSFERASE

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y63-025 ◽

1963 ◽

Vol 41 (1) ◽

pp. 201-210 ◽

Cited By ~ 2

Author(s):

J. Blumenstein ◽

G. R. Williams

Keyword(s):

Secondary Amine ◽

Liver Homogenate ◽

Supernatant Fraction ◽

Biological Oxidation ◽

Methyl Group

The supernatant fraction of liver homogenate brings about the methylation of glycine by S-adenosylmethionine. The secondary amine formed in the reaction appears to be sarcosine. This conclusion is based on (I) the stoichiometry of the reaction, and (II) radioautography of chromatograms of the incubation products. The enzyme, glycine N-methyltransferase, was purified 80-fold, and certain of its characteristics are reported. The possible role of the enzyme in the biological oxidation of the methyl group of methionine is discussed.

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Influence of attractants and repellents on methyl group turnover on methyl-accepting chemotaxis proteins of Bacillus subtilis and role of CheW.

Journal of Bacteriology ◽

10.1128/jb.174.13.4218-4222.1992 ◽

1992 ◽

Vol 174 (13) ◽

pp. 4218-4222 ◽

Cited By ~ 11

Author(s):

D W Hanlon ◽

P B Carpenter ◽

G W Ordal

Keyword(s):

Bacillus Subtilis ◽

Methyl Group ◽

Chemotaxis Proteins

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On the quantitative relations between structure and antiaggregation activity of ω-aryl-ω-oxoalkanoic acids

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19862617 ◽

1986 ◽

Vol 51 (11) ◽

pp. 2617-2625 ◽

Cited By ~ 6

Author(s):

Miroslav Kuchař ◽

Bohumila Brunová ◽

Jaroslava Grimová ◽

Václav Rejholec ◽

Václav Čepelák

Keyword(s):

Partition Coefficients ◽

Dissociation Constants ◽

Inhibitory Effect ◽

Partition Chromatography ◽

Linear Quadratic ◽

Marked Influence ◽

Methyl Group ◽

Retention Characteristics ◽

Anti Inflammatory

A series of ω-aryl-ω-oxoalkanoic acids, I-IV, has been prepared and investigated for dissociation constants in 80% methylcellosolve, retention characteristics in thin-layer partition chromatography and partition coefficients P in the system octanol-water. Also evaluated were their anti-inflammatory efficacy and inhibitory effect on the platelet aggregation induced by collagen. Analysing the relations between structure and antiaggregation effect, we obtained a non-linear, quadratic dependence of this effect on lipophilicity, the optimum being at log P = 3. The antiaggregation effect increased with shortening the chain between the carbonyl and the carboxyl, and with increasing acidity. It was also diminished by the presence of a methyl group on the interlinking chain. To assess the role of lipophilicity we used the RM values of partition chromatography. The relation between anti-inflammatory efficacy and structure was assessed only qualitatively. In this aspect, too, the nature of the chain between the carbonyl and carboxyl proved to have a marked influence. The anti-inflammatory activity proved considerably enhanced by the presence of another aromatic ring in ω-oxoalkanoic acids derived from biphenyl.

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Effects of Several Androgens and Steroid Metabolites on Erythropoietin Production in the Isolated Perfused Dog Kidney

Blood ◽

10.1182/blood.v43.1.39.39 ◽

1974 ◽

Vol 43 (1) ◽

pp. 39-47 ◽

Cited By ~ 24

Author(s):

Luiz G. Paulo ◽

Gregory D. Fink ◽

Byung L. Roh ◽

James W. Fisher

Keyword(s):

Double Bond ◽

Spatial Configuration ◽

Significant Elevation ◽

Methyl Group ◽

Erythropoietin Production ◽

Dog Kidney ◽

Isolated Kidneys

Abstract In an attempt to clarify the role of the kidney in the action of several androgens and steroid metabolites on erythropoietin (ESF) production, ESF titers were measured in perfusates of isolated kidneys from dogs previously exposed to 4-hr hypoxia and perfused with blood containing testosterone (Test), 5α-17β-hydroxyandrostan-3-one (5αDHT), 5β-17β-hydroxyandrostan-3-one (5βDHT), 19-nortestosterone (19-nor), oxymetholone (Oxy), fluoxymesterone (Fluoxy), 3α-hydroxy-5β-pregnane, 11,20-dione (3αOH5βpreg), or 3β-hydroxy-5β-pregnane-20-one (3βOH-5 preg). ESF levels in the perfusates were assayed in exhypoxic polycythemic mice. Test, 5αDHT, oxy, fluoxy, and 3βOH5β-preg were found to produce a significant increase in ESF levels in the kidney perfusates. 5βDHT, 19-nor, and 3αOH5βpreg failed to produce a significant elevation in erythropoietin titers in the perfusates of the isolated perfused kidneys. These data suggest that the 4-5 double bond and the spatial configuration of the hydrogen at the 5 position as well as the lack of a methyl group in the 19 position of the basic androstan nucleus are important in the ability of these steroids to stimulate kidney production of ESF.

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