Morphological development in vitro of the whole and halved lower molar tooth germ of the mouse

1971 ◽  
Vol 16 (12) ◽  
pp. 1481-IN13 ◽  
Author(s):  
Audrey R. Fisher
Keyword(s):  
Tooth Germ ◽  
Molar Tooth ◽  
Morphological Development ◽  
Development In Vitro ◽  
Lower Molar

Tunicamycin inhibits mouse tooth morphogenesis and odontoblast differentiation in vitro

Development ◽  
1980 ◽  
Vol 58 (1) ◽  
pp. 195-208
Author(s):  
Irma Thesleff ◽  
Robert M. Pratt
Keyword(s):  
Basement Membrane ◽  
Tooth Germ ◽  
Mesenchymal Cells ◽  
Protein Glycosylation ◽  
Molar Tooth ◽  
Odontoblast Differentiation ◽  
Matrix Interaction ◽  
Cell Matrix ◽  
Dose Dependent

Tunicamycin (TM), an antibiotic that selectively inhibits dolichol-mediated protein glycosylation, inhibited morphogenesis and differentiation of odontoblasts in the molar tooth germ in vitro. These effects of TM are reversible and dose-dependent, and in advanced teeth the effect of TM was not complete unless the basement membrane was removed prior to culture. TM did not prevent secretion of predentin or enamel when added to the cultures after initiation of predentin secretion. TM dramatically inhibited protein glycosylation and the accumulation of labeled proteoglycans and glycoproteins in the basement membrane. Our previous studies indicated that odontoblast differentiation is triggered by an interaction between the basement membrane and mesenchymal cells. We suggest that TM inhibits odontoblast differentiation by causing alterations in the basement membrane which prevent the necessary cell-matrix interaction required for odontoblast differentiation.

A staging scheme for assessing development in vitro of organogenesis stage embryos of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: dasyuridae)

Reproduction ◽  
2000 ◽  
pp. 99-108 ◽  
Author(s):  
YP Cruz ◽  
D Hickford ◽  
L Selwood
Keyword(s):  
Fetal Calf Serum ◽  
Calf Serum ◽  
Microscopic Analysis ◽  
Culture Conditions ◽  
Didelphis Virginiana ◽  
Morphological Development ◽  
Rat Serum ◽  
Sminthopsis Macroura ◽  
Development In Vitro

The inaccessibility of mammalian organogenesis stage embryos has precluded their widespread use in embryological and teratological studies. As organogenesis occurs during the last 1.5 days of the 10. 7 days of gestation in the stripe-faced dunnart (Sminthopsis macroura), the aim of the present study was to investigate whether day 9 and day 10 embryos and fetuses could be grown to term in vitro. High glucose Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS) supported embryonic growth for various periods of time, some to within 5 h of the predicted time of parturition. A roller culture system maintained at 35 degrees C was used to incubate organogenesis stage embryos (n = 43). Nine unincubated (control) embryos were either fixed for microscopic analysis or frozen for microprotein determination. The results of the present study indicate that with some optimization of the culture conditions (increasing oxygen in the gas phase in the culture tubes, replacing FCS with rat serum), it might be possible for organogenesis stage S. macroura embryos to be grown to term. A scoring scheme for assessing morphological development was devised for use as a standard in staging organogenesis stage embryos. This scheme reflects the highly compressed schedule of developmental events that occurs mainly during day 9 of gestation in S. macroura embryos. In comparison, during embryogenesis in Didelphis virginiana these developmental events occur from day 8 to day 10.5 of gestation, and birth occurs on day 13.

Expression, localisation and synthesis of versican by the enamel organ of developing mouse molar tooth germ: An in vivo and in vitro study

2010 ◽  
Vol 55 (12) ◽  
pp. 995-1006 ◽  
Author(s):  
Bei-Zhan Jiang ◽  
Tamaki Yokohama-Tamaki ◽  
Zuo-lin Wang ◽  
Nobuko Obara ◽  
Shunichi Shibata
Keyword(s):  
In Vitro Study ◽  
Tooth Germ ◽  
Vitro Study ◽  
Molar Tooth ◽  
Enamel Organ

Effect of adriamycin on hamster molar tooth development in vitro: 1. Morphological changes

1989 ◽  
Vol 225 (4) ◽  
pp. 318-328 ◽  
Author(s):  
A. C. Karim ◽  
J. H. M. Woltgens ◽  
Th. J. M. Bervoets ◽  
D. M. Lyaruu ◽  
A. L. J. J. Bronckers
Keyword(s):  
Tooth Development ◽  
Morphological Changes ◽  
Molar Tooth ◽  
Development In Vitro

Daunorubicin-Induced Pathology in the Developing Hamster Molar Tooth Germ in Vitro

1999 ◽  
Vol 23 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Lyaruu ◽  
van Duin ◽  
Bervoets ◽  
Bronckers ◽  
Woltgens
Keyword(s):  
Tooth Germ ◽  
Molar Tooth

Stages of neuronal morphological development in vitro—An automated assay

2011 ◽  
Vol 199 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Michelle K. Leach ◽  
Youssef I. Naim ◽  
Zhang-Qi Feng ◽  
Caitlyn C. Gertz ◽  
Joseph M. Corey
Keyword(s):  
Morphological Development ◽  
Automated Assay ◽  
Development In Vitro

Influence of corticosteroids and thyroxine on lung development in vitro

1985 ◽  
Vol 110 (1_Suppla) ◽  
pp. S35-S36 ◽  
Author(s):  
B. ZIMMERMANN
Keyword(s):  
Lung Development ◽  
Development In Vitro

The effect of development in vitro

2014 ◽  
Author(s):  
Hitomi Obata ◽  
Maki Kamoshita ◽  
Tsubasa Kato ◽  
Junya Ito ◽  
Naomi Kashiwazaki
Keyword(s):  
Development In Vitro
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