Insulin and insulinlike growth factors in embryonic development. Effects of a biologically inert insulin (guinea pig) on rat embryonic growth and development in vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

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Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.87.12.4756 ◽

1990 ◽

Vol 87 (12) ◽

pp. 4756-4760 ◽

Cited By ~ 382

Author(s):

B. C. Paria ◽

S. K. Dey

Keyword(s):

Growth Factors ◽

Embryo Development ◽

Preimplantation Embryo ◽

Preimplantation Embryo Development ◽

Cooperative Interactions ◽

Development In Vitro

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High doses of lipid-core nanocapsules do not affect bovine embryonic development in vitro

Toxicology in Vitro ◽

10.1016/j.tiv.2017.09.013 ◽

2017 ◽

Vol 45 ◽

pp. 194-201 ◽

Cited By ~ 4

Author(s):

Caroline G. Lucas ◽

Mariana H. Remião ◽

Franciele A. Bruinsmann ◽

Isadora A.R. Lopes ◽

Morgana A. Borges ◽

...

Keyword(s):

Embryonic Development ◽

Lipid Core ◽

Development In Vitro ◽

High Doses

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Peptide-containing neurons in explant cultures of guinea-pig myenteric plexus during development in vitro: Gross morphology and growth patterns

Cell and Tissue Research ◽

10.1007/bf00220030 ◽

1988 ◽

Vol 254 (1) ◽

Cited By ~ 9

Author(s):

M.J. Saffrey ◽

G. Burnstock

Keyword(s):

Guinea Pig ◽

Myenteric Plexus ◽

Growth Patterns ◽

Explant Cultures ◽

Development In Vitro

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In vitro analysis of glucose metabolism and embryonic growth in postimplantation rat embryos

Development ◽

10.1242/dev.100.3.431 ◽

1987 ◽

Vol 100 (3) ◽

pp. 431-439 ◽

Cited By ~ 1

Author(s):

S.K. Ellington

Keyword(s):

Glucose Metabolism ◽

Embryonic Development ◽

Glucose Uptake ◽

Glucose Concentration ◽

Culture Media ◽

Rat Embryos ◽

Embryonic Growth ◽

Stage Of Development

The glucose metabolism and embryonic development of rat embryos during organogenesis was studied using embryo culture. Glucose uptake and embryonic growth and differentiation of 10.5-day explants (embryos + membranes) were limited by the decreasing glucose concentration, but not the increasing concentration of metabolites, in the culture media during the second 24 h of a 48 h culture. No such limitations were found on the embryonic development of 9.5-day explants during a 48 h culture although glucose uptake was slightly reduced at very low concentrations of glucose. From the head-fold stage to the 25-somite stage of development, glucose uptake was characteristic of the stage of development of the embryo and not the time it had been in culture. Embryonic growth of 9.5-day explants was similar to that previously observed in vivo. Glucose uptake by 9.5-day explants was dependent on the surface area of the yolk sac and was independent of the glucose concentration in the culture media (within the range of 9.4 to 2.5 mM). The proportion of glucose converted to lactate was 100% during the first 42h of culture then fell to about 50% during the final 6h. The protein contents of both the extraembryonic membranes and the embryo were dependent on the glucose uptake.

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Human embryonic development in vitro

In Vitro Maturation of Human Oocytes ◽

10.1201/b14636-58 ◽

2006 ◽

pp. 316-328

Keyword(s):

Embryonic Development ◽

Development In Vitro

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Recombinant human megakaryocyte growth and development factor stimulates thrombocytopoiesis in normal nonhuman primates

Blood ◽

10.1182/blood.v86.1.54.bloodjournal86154 ◽

1995 ◽

Vol 86 (1) ◽

pp. 54-59 ◽

Cited By ~ 145

Author(s):

AM Farese ◽

P Hunt ◽

T Boone ◽

TJ MacVittie

Keyword(s):

Bone Marrow ◽

Peripheral Blood ◽

Growth And Development ◽

Nonhuman Primates ◽

Normal Male ◽

Platelet Counts ◽

Development Factor ◽

Development In Vitro

Megakaryocyte growth and development factor (MGDF) is a novel cytokine that binds to the c-mpl receptor and stimulates megakaryocyte development in vitro and in vivo. This report describes the ability of recombinant human (r-Hu) MGDF to affect megakaryocytopoiesis in normal nonhuman primates. r-HuMGDF was administered subcutaneously to normal, male rhesus monkeys once per day for 10 consecutive days at dosages of 2.5, 25, or 250 micrograms/kg of body weight. Bone marrow and peripheral blood were assayed for clonogenic activity and peripheral blood counts were monitored. Circulating platelet counts increased significantly (P < .05) for all doses within 6 days of r-HuMGDF administration and reached maximal levels between day 12 and day 14 postcytokine administration. The 2.5, 25.0, and 250.0 micrograms/kg/d doses elicited peak mean platelet counts that were 592%, 670%, and 449% of baseline, respectively. Bone marrow-derived clonogenic data showed significant increases in the concentration of megakaryocyte (MEG)- colony-forming unit (CFU) and granulocyte-erythroid-macrophage- megakaryocyte (GEMM)-CFU, whereas that of granulocyte-macrophage (GM)- CFU and burst-forming unit-erythroid (BFU-e) remained unchanged during the administration of r-HuMGDF. These data show that r-HuMGDF is a potent stimulator of thrombocytopoiesis in the normal nonhuman primate.

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