Effect of lincomycin on the chlorophyll protein complex I content and Photosystem I activity of greening leaves

1977 ◽  
Vol 460 (3) ◽  
pp. 431-444 ◽  
Author(s):  
Roger G. Hiller ◽  
Theodora B.G. Pilger ◽  
Sandra Genge
Keyword(s):  
Photosystem I ◽  
Protein Complex ◽  
Complex I ◽  
Chlorophyll Protein

scholarly journals Functional and structural changes in chloroplasts of senescent tobacco leaves

2015 ◽  
Vol 45 (3) ◽  
pp. 341-352 ◽  
Author(s):  
Danuta Wolińska
Keyword(s):  
Electron Microscope ◽  
Photosystem I ◽  
Complex I ◽  
Structural Changes ◽  
Photosynthetic Activity ◽  
Chloroplast Structure ◽  
Tobacco Leaves ◽  
Stroma Lamellae ◽  
Chlorophyll Protein ◽  
The Relationship

The relationship between the structure and the function of chloroplasts of aging tobacco leaves was studied. From the observations of chloroplast structure in electron microscope, the results of other authors concerning earlier degradation of stroma lamellae in comparison with grana were confirmed. It was shown that this process is accompanied by changes in the content of galactolipids (in the first place MGDG) and earlier decomposition of chlorophyll-protein-complex I. This resulted in decrease of photosynthetic activity of leaves and earlier disappearance of Photosystem I activity.

scholarly journals A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii.

1975 ◽  
Vol 67 (2) ◽  
pp. 361-377 ◽  
Author(s):  
N H Chua ◽  
K Matlin ◽  
P Bennoun
Keyword(s):  
Molecular Weight ◽  
Chlamydomonas Reinhardtii ◽  
Photosystem I ◽  
Protein Complex ◽  
Protein Complexes ◽  
Sodium Dodecyl ◽  
Weight Ratio ◽  
Thylakoid Membranes ◽  
Higher Plant ◽  
Chlorophyll Protein

Sodium dodecyl sulfate gel electrophoresis of unheated, detergent-solubilized thylakoid membranes of Chlamydomonas reinhardtii gives two chlorophyll-protein complexes. Chlorophyll-protein complex I (CP I) is the blue-green in color and can be dissociated by heat into "free" chlorophyll and a constituent polypeptide (polypeptide 2; mol wt 66,000). Similar experiments with spinach and Chinese cabbage show that the higher plant CP I contains an equivalent polypeptide but of slightly lower molecular weight (64,000). Both polypeptide 2 and its counterpart in spinach are soluble in a 2:1 (vol/vol) mixture of chloroform-methanol. Chemical analysis reveals that C. reinhardtii CP I has a chlorophyll a to b weight ratio of about 5 and that it contains approximately 5% of the total chlorophyll and 8-9% of the total protein of the thylakoid membranes. Thus, it can be calculated that each constituent polypeptide chain is associated with eight to nine chlorophyll molecules. Attempts to measure the molecular weight of CP I by calibrated SDS gels were unsuccessul since the complex migrates anomalously in such gels. Two Mendelian mutants of C. reinhardtii, F1 and F14, which lack P700 but have normal photosystem I activity, do not contain CP I or the 66,000-dalton polypeptide in their thylakoid membranes. Our results suggest that CP I is essential for photosystem I reaction center activity and that P700 may be associated with the 66,000-dalton polypeptide.

Distribution of cis-trans-β-Carotene in Pigment-Protein Fractions of Photosystem I and II

1988 ◽  
Vol 43 (7-8) ◽  
pp. 577-580
Author(s):  
S. S. Brody ◽  
D. Simpson ◽  
M. Rieh
Keyword(s):  
Photosystem Ii ◽  
Photosystem I ◽  
Protein Complex ◽  
Complex I ◽  
Protein Fractions ◽  
Ps I ◽  
Pigment Protein Complex ◽  
Photosystem I And Ii ◽  
Β Carotene

The distribution of isomers of β-carotene is reported in the CP-47 pigment-protein complex of photosystem II and photosystem I (PS I) from barley. The distribution of carotenoids from light harvester complex I I is reported. CP-47 lacks 9,9′-rá-carotene, but contains 9,13′-cis-carotene; PS I lacks 9,13′-cis but contains 9,9′-eis. More 9-cis, 9,13-cis, 9,15-cis, and epoxide is observed in CP-47 than in PS I.

Sign in / Sign up

Export Citation Format

Share Document