Separation and reconstitution of sodium-dependent glucose transport activity from renal brush-border membranes using gel-filtration chromatography

1986 ◽  
Vol 858 (1) ◽  
pp. 83-91 ◽  
Author(s):  
J.C. Poirée ◽  
M. Starita-Geribaldi ◽  
P. Sudaka
Keyword(s):  
Glucose Transport ◽  
Brush Border ◽  
Gel Filtration ◽  
Transport Activity ◽  
Gel Filtration Chromatography ◽  
Brush Border Membranes ◽  
Sodium Dependent ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border

scholarly journals A rapid method for the reconstitution of Na+-dependent neutral amino acid transport from bovine renal brush-border membranes

1987 ◽  
Vol 244 (3) ◽  
pp. 503-508 ◽  
Author(s):  
A M Lynch ◽  
J D McGivan
Keyword(s):  
Amino Acid ◽  
Rapid Method ◽  
Brush Border ◽  
Amino Acid Transport ◽  
Neutral Amino Acid ◽  
Acid Transport ◽  
Transport Activity ◽  
Brush Border Membranes ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border

1. A simple and rapid method for the reconstitution of Na+-dependent neutral amino acid transport activity from bovine renal brush border membranes is described. 2. The neutral detergent decanoyl-N-methylglucamide (‘MEGA-10’) was employed to solubilize the membrane protein. This obviated the necessity for a prolonged dialysis step. 3. The properties of amino acid transport in these vesicles were similar to those observed in native membranes. 4. This should be a useful procedure in the eventual identification and isolation of amino acid transport proteins.

scholarly journals PHOSPHATE (Pi) TRANSPORT IN RENAL BRUSH BORDER MEMBRANES (BBMV) IN NEWBORN PUPPIES (P)

1984 ◽  
Vol 18 ◽  
pp. 366A-366A
Author(s):  
Eddie S Moore ◽  
Eunice G John ◽  
Lawrence Rufr ◽  
Christine S Mooers ◽  
Nochik Park ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membranes ◽  
Pi Transport ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border
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