Porphyrin biosynthesis in soybean callus tissue system. Isolation, purification and general properties of δ-aminolaevulinate dehydratase

1968 ◽  
Vol 151 (1) ◽  
pp. 300-302 ◽  
Author(s):  
H.A. Tigier ◽  
A.M.del C. Batlle ◽  
G. Locascio
Keyword(s):  
Callus Tissue ◽  
Soybean Callus ◽  
General Properties ◽  
Tissue System

Porphyrin biosynthesis in the soybean callus tissue system—XV. The effect of growth conditions

1975 ◽  
Vol 6 (8) ◽  
pp. 591-606 ◽  
Author(s):  
Alcira M. Del C. Batlle ◽  
Elena B.C. Llambias ◽  
Eva Wider de Xlfra ◽  
Horacio A. Tigier
Keyword(s):  
Callus Tissue ◽  
Growth Conditions ◽  
Soybean Callus ◽  
Tissue System

scholarly journals Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division

1990 ◽  
Vol 265 (3) ◽  
pp. 809-814 ◽  
Author(s):  
M Biffen ◽  
D E Hanke
Keyword(s):  
Glycine Max ◽  
Cell Division ◽  
Suspension Culture ◽  
Callus Tissue ◽  
Plant Tissues ◽  
Intracellular Level ◽  
Soybean Callus ◽  
Inositol 1 ◽  
Division Cell ◽  
Important Enzyme

Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol has not been demonstrated. We have made use of deoxyglucose to reduce intracellular levels of myo-inositol. Deoxyglucose is phosphorylated to deoxyglucose 6-phosphate, which inhibits L-myo-inositol 1-phosphate synthase, an important enzyme in the synthesis of myo-inositol. Addition of deoxyglucose to the medium resulted in a decrease in the intracellular level of myo-inositol that corresponded with a decrease in cell division. Cell viability was not affected. When myo-inositol was added to cells along with deoxyglucose, cell division was restored, as were intracellular levels of myo-inositol. Addition of myo-inositol had no affect on the uptake or metabolism of deoxyglucose. From these results we propose that myo-inositol has a role in maintaining cell division in soybean callus tissue in suspension culture.

The stability and biological activity of cytokinin metabolites in soybean callus tissue

Planta ◽  
1984 ◽  
Vol 160 (3) ◽  
pp. 242-249 ◽  
Author(s):  
L. M. S. Palni ◽  
M. V. Palmer ◽  
D. S. Letham
Keyword(s):  
Biological Activity ◽  
Callus Tissue ◽  
Soybean Callus ◽  
The Stability
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