Post-heparin lipolytic activity with no hepatic triacylglycerol lipase involved in a mammalian species

1981 ◽  
Vol 663 (2) ◽  
pp. 516-523 ◽  
Author(s):  
Jacqueline Etienne ◽  
Lydie Noé ◽  
Monique Rossignol ◽  
Anne-Marie Dosne ◽  
Jacques Debray
Keyword(s):  
Lipolytic Activity ◽  
Mammalian Species ◽  
Triacylglycerol Lipase ◽  
Post Heparin Lipolytic Activity ◽  
Hepatic Triacylglycerol

Stimulation of the activity and mRNA level of hepatic triacylglycerol lipase by triiodothyronine in HepG2 cells

1992 ◽  
Vol 1127 (3) ◽  
pp. 298-302 ◽  
Author(s):  
Shuichi Nozaki ◽  
Iichiro Shimomura ◽  
Tohru Funahashi ◽  
Masakazu Menju ◽  
Masaharu Kubo ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Mrna Level ◽  
Triacylglycerol Lipase ◽  
Hepatic Triacylglycerol ◽  
Stimulation Of

Pancreatic triacylglycerol lipase in a hibernating mammal. II. Cold-adapted function and differential expression

2003 ◽  
Vol 16 (1) ◽  
pp. 131-140 ◽  
Author(s):  
Teresa L. Squire ◽  
Mark E. Lowe ◽  
Vernon W. Bauer ◽  
Matthew T. Andrews
Keyword(s):  
Low Temperature ◽  
High Activity ◽  
Ground Squirrel ◽  
Mammalian Cells ◽  
Lipolytic Activity ◽  
Tissue Expression ◽  
Ground Squirrels ◽  
Mrna Levels ◽  
Western Blots ◽  
Triacylglycerol Lipase

Thirteen-lined ground squirrels ( Spermophilus tridecemlineatus) exploit the low-temperature activity of pancreatic triacylglycerol lipase (PTL) during hibernation. Lipolytic activity at body temperatures associated with hibernation was examined using recombinant ground squirrel and human PTLs expressed in yeast. Both the human and ground squirrel enzymes displayed high activity at temperatures as low as 0°C and showed Q10 values of 1.2–1.5 over a range of 37–7°C. These studies indicate that low-temperature lipolysis is a general property of PTL and does not require protein modifications unique to mammalian cells and/or the hibernating state. Western blots show elevated levels of PTL protein during hibernation in both heart and white adipose tissue (WAT). Significant increases in PTL gene expression are seen in heart, WAT, and testes; but not in pancreas, where PTL mRNA levels are highest. Upregulation of PTL in testes is also accompanied by expression of the PTL-specific cofactor, colipase. The multi-tissue expression of PTL during hibernation supports its role as a key enzyme that shows high activity at low temperatures.

scholarly journals Lipid Lowering Activity ofAnthocephalus indicusRoot in Hyperlipidemic Rats

2010 ◽  
Vol 7 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Vishnu Kumar ◽  
Mohammad Mubin Khan ◽  
Ashok Kumar Khanna ◽  
Ranjana Singh ◽  
Sushma Singh ◽  
...  
Keyword(s):  
Hydroxyl Radicals ◽  
Plasma Lipids ◽  
Antioxidant Activities ◽  
Lipolytic Activity ◽  
Lipid Lowering ◽  
Root Extract ◽  
Superoxide Anions ◽  
Post Heparin Lipolytic Activity ◽  
Lowering Activity

The lipid lowering activity ofAnthocephalus indicus(family Rubiaceae; Hindi name Kadamba) root extract has been studied in triton WR-1339 induced hyperlipidemia in rats. In this model, feeding with root extract (500 mg kg−1b.w.) lowered plasma lipids and reactivated post-heparin lipolytic activity in hyperlipidemic rats. Furthermore, the root extract (50–500 μM) inhibited the generation of superoxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems,in vitro. The results of the present study demonstrated both lipid lowering and antioxidant activities in root extract ofA. indicus, which could help prevention of hyperlipidemia and related diseases.

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