scholarly journals Lipid Lowering Activity ofAnthocephalus indicusRoot in Hyperlipidemic Rats

2010 ◽  
Vol 7 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Vishnu Kumar ◽  
Mohammad Mubin Khan ◽  
Ashok Kumar Khanna ◽  
Ranjana Singh ◽  
Sushma Singh ◽  
...  
Keyword(s):  
Hydroxyl Radicals ◽  
Plasma Lipids ◽  
Antioxidant Activities ◽  
Lipolytic Activity ◽  
Lipid Lowering ◽  
Root Extract ◽  
Superoxide Anions ◽  
Post Heparin Lipolytic Activity ◽  
Lowering Activity

The lipid lowering activity ofAnthocephalus indicus(family Rubiaceae; Hindi name Kadamba) root extract has been studied in triton WR-1339 induced hyperlipidemia in rats. In this model, feeding with root extract (500 mg kg−1b.w.) lowered plasma lipids and reactivated post-heparin lipolytic activity in hyperlipidemic rats. Furthermore, the root extract (50–500 μM) inhibited the generation of superoxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems,in vitro. The results of the present study demonstrated both lipid lowering and antioxidant activities in root extract ofA. indicus, which could help prevention of hyperlipidemia and related diseases.

Study of Antihyperglycemic, Antihyperlipidemic and Antioxidant Activities of Tannins Extracted from Warionia saharae Benth. & Coss

2019 ◽  
Vol 19 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Mohammed Ajebli ◽  
Fadwa El Ouady ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Soxhlet Apparatus

Background and Objective: Warionia saharae Benth & Coss, a plant belonging to Asteraceae family, is used for its anti-diabetic properties in Morocco. The objective of this study was to evaluate the effect of tannins extracted from Warionia saharae (W. saharae) on blood glucose levels and lipid profile in normal and streptozotocin(STZ)-induced diabetic rats. Methods: Tannins (TE) were extracted from W. saharae using Soxhlet apparatus and different organic solvents. Single and once daily repeated oral administration of TE (10 mg/kg) for 15 days were used to evaluate the glucose and lipid-lowering activity in normal and diabetic rats. Furthermore, glucose test tolerance, liver histopathological examination and in vitro antioxidant activity of TE were carried out in this study. Results: The results showed that TE was able to exert antihyperglycemic and lowering total cholesterol effects as well as improvement of the high-density lipoprotein (HDL)-cholesterol serum level after 15 days of treatment. Furthermore, TE improved glucose tolerance, histopathological status of liver in diabetic rats and demonstrated interesting antioxidant activity. Conclusion: In conclusion, the present investigation revealed that TE possesses potent antidiabetic and antihyperlipidemic activities as claimed in different ethnopharmacological practices.

scholarly journals Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

2018 ◽  
Vol 4 (3) ◽  
pp. 114-119
Author(s):  
Quang Ung Le ◽  
◽  
Horng Liang Lay ◽  
Ming Chang Wu ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Total Phenolic ◽  
Root Extract ◽  
Inhibitory Capacity ◽  
Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

scholarly journals Antioxidant and Proapoptotic Activities ofSclerocarya birrea[(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Maria Francesca Armentano ◽  
Faustino Bisaccia ◽  
Rocchina Miglionico ◽  
Daniela Russo ◽  
Nicoletta Nolfi ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Antioxidant Activities ◽  
Dermal Fibroblasts ◽  
Root Extract ◽  
Dependent Manner ◽  
Mitochondrial Membrane Depolarization

The main goal of this study was to characterize thein vitroantioxidant activity and the apoptotic potential ofS. birreamethanolic root extract (MRE). Among four tested extracts, obtained with different solvents, MRE showed the highest content of polyphenols, flavonoids, and tannins together with antioxidant activities tested with superoxide, nitric oxide, ABTS, and beta-carotene bleaching assays. Moreover, the cytotoxic effect of MRE was evaluated on the hepatocarcinoma cell line HepG2. In these cells, MRE treatment induced apoptosis and generated reactive oxygen species (ROS) in dose-dependent manner. The cytotoxic effect promoted by MRE was prevented by pretreatment of HepG2 cells with N-acetyl-L-cysteine (NAC), suggesting that oxidative stress was pivotal in MRE-mediated cell death. Moreover, we showed that the MRE treatment induced the mitochondrial membrane depolarization and the cytochromecrelease from mitochondria into the cytosol. It suggests that the apoptosis occurred in a mitochondrial-dependent pathway. Interestingly, MRE showed a sensibly lower cytotoxicity, associated with a low increase of ROS, in normal human dermal fibroblasts compared to HepG2 cells. It is suggested that the methanolic root extract ofS. Birreais able to selectively increase intracellular ROS levels in cancer cells, promoting cell death.

Experimental Investigation on the Lipid-Lowering Activity of Three Novel Antilipidemic Materials In Vitro

2011 ◽  
Vol 399-401 ◽  
pp. 1568-1572 ◽  
Author(s):  
Si Rong Tan ◽  
Si Hui Wu ◽  
Bing Gao ◽  
Jun Rui Xiao ◽  
Zi Yue Guo ◽  
...  
Keyword(s):  
Particle Size ◽  
Physical Adsorption ◽  
Food Additives ◽  
Chitosan Nanoparticles ◽  
Lipid Lowering ◽  
Particle Sizes ◽  
Particle Size Analyzer ◽  
Determinant Factor ◽  
Lowering Activity

Chitosan is a new kind of material in life-science, it can be used in lipid-lowering. Chitosan microspheres (CTS-MP) were prepared by spray-drying, chitosan nanoparticles (CTS-NP) and chitosan-capsaicin microspheres (CTS-CAP-MP) were prepared by ionic gelation process. The effect of these samples on lowering lipids was evaluated by measuring its’ binding capacities with lipids under the conditions mimicking the gastrointestinal tract in vitro. Also, the particle sizes of chitosan preparations were examined by scanning electron microscopy (SEM) and particle size analyzer. The results showed that the lipids-binding capacities of CTS-MP and CTS-NP were far more higher than that of CTS and CTS-CAP-MP. In conclusion, CTS-MP and CTS-NP will be better fat-lowing food additives to replace chitosan. The mechanism of lipids-binding in vitro is not only physical adsorption. Particle size is not the determinant factor, electrostatic action is maybe another factor of lipids-binding.

scholarly journals The Positive Association between Plasma Myristic Acid and ApoCIII Concentrations in Cardiovascular Disease Patients Is Supported by the Effects of Myristic Acid in HepG2 Cells

2020 ◽  
Vol 150 (10) ◽  
pp. 2707-2715 ◽  
Author(s):  
Oliviero Olivieri ◽  
Giulia Speziali ◽  
Annalisa Castagna ◽  
Patrizia Pattini ◽  
Silvia Udali ◽  
...  
Keyword(s):  
Myristic Acid ◽  
Hepg2 Cells ◽  
Plasma Lipids ◽  
Plasma Concentrations ◽  
Plasma Lipid ◽  
Positive Association ◽  
Fold Increase ◽  
Lipid Lowering ◽  
In Vitro Experiments

ABSTRACT Background In the settings of primary and secondary prevention for coronary artery disease (CAD), a crucial role is played by some key molecules involved in triglyceride (TG) metabolism, such as ApoCIII. Fatty acid (FA) intake is well recognized as a main determinant of plasma lipids, including plasma TG concentration. Objectives The aim was to investigate the possible relations between the intakes of different FAs, estimated by their plasma concentrations, and circulating amounts of ApoCIII. Methods Plasma samples were obtained from 1370 subjects with or without angiographically demonstrated CAD (mean ± SD age: 60.6 ± 11.0 y; males: 75.8%; BMI: 25.9 ± 4.6 kg/m2; CAD: 73.3%). Plasma lipid, ApoCIII, and FA concentrations were measured. Data were analyzed by regression models adjusted for FAs and other potential confounders, such as sex, age, BMI, diabetes, smoking, and lipid-lowering therapies. The in vitro effects of FAs were tested by incubating HepG2 hepatoma cells with increasing concentrations of selected FAs, and the mRNA and protein contents in the cells were quantified by real-time RT-PCR and LC-MS/MS analyses. Results Among all the analyzed FAs, myristic acid (14:0) showed the most robust correlations with both TGs (R = 0.441, P = 2.6 × 10−66) and ApoCIII (R = 0.327, P = 1.1 × 10−31). By multiple regression analysis, myristic acid was the best predictor of both plasma TG and ApoCIII variability. Plasma TG and ApoCIII concentrations increased progressively at increasing concentrations of myristic acid, independently of CAD diagnosis and gender. Consistent with these data, in the in vitro experiments, an ∼2-fold increase in the expression levels of the ApoCIII mRNA and protein was observed after incubation with 250 μM myristic acid. A weaker effect (∼30% increase) was observed for palmitic acid, whereas incubation with oleic acid did not affect ApoCIII protein or gene expression. Conclusions Plasma myristic acid is associated with increased ApoCIII concentrations in cardiovascular patients. In vitro experiments indicated that myristic acid stimulates ApoCIII expression in HepG2 cells.

Antioxidant activities of the new tetrasubstituted metal-free, Zn(II) and Co(II) monophthalocyanines

2018 ◽  
Vol 22 (01n03) ◽  
pp. 233-242 ◽  
Author(s):  
Nazlı Söylemez ◽  
Ebru Yabaş ◽  
Serap Şahin Bölükbaşı ◽  
Mustafa Sülü
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radicals ◽  
Antioxidant Activities ◽  
High Solubility ◽  
Metal Free ◽  
Tert Butyl ◽  
H Nmr ◽  
Aggregation Behaviors ◽  
C Nmr

4-[(4′-(Tert-butyl)phenoxy)phenoxy]phthalonitrile 1 has been prepared by the reaction of 4-(4-nitrophenoxy)phthalonitrile with 4-tertiarylbutylphenole. 4-((4′-tert-butyl)phenoxy)phenoxy tetrasubstituted metal-free 2, zinc(II) 3 and cobalt(II) 4 phthalocyanines have been prepared by tetramerization of compound 1. The synthesized phthalocyanines showed high solubility in common organic solvents such as CHCl[Formula: see text]. All compounds were characterized by elemental analysis and [Formula: see text]H-NMR, [Formula: see text]C-NMR, UV-vis, IR spectra. Aggregation behaviors of these compounds have been investigated in different solvents (CHCl[Formula: see text], THF, DMF and DMSO) and different concentrations in CHCl[Formula: see text]. The in vitro antioxidant activities of phthalocyanine compounds 2, 3 and 4 were evaluated in a series of assays involving DPPH radicals, hydroxyl radicals, superoxide radicals, singlet oxygen and hydrogen peroxide. Antioxidant activity of compound 2was found to be higher than that of compounds 3 and 4.

scholarly journals In vitro antioxidant activities of root extract of Asparagus racemosus Linn.

2018 ◽  
Vol 8 (1) ◽  
pp. 60-65 ◽  
Author(s):  
D.S. Karuna ◽  
Prasanta Dey ◽  
Shibu Das ◽  
Amit Kundu ◽  
Tejendra Bhakta
Keyword(s):  
Antioxidant Activities ◽  
Root Extract ◽  
Asparagus Racemosus

scholarly journals Comparative Phytochemical and in vitro Antioxidant Screening of the Root and Stem Bark of Annona muricata Linn

2020 ◽  
pp. 48-61
Author(s):  
C. E. Anarado ◽  
F. M. Chukwubueze ◽  
C. J. O. Anarado ◽  
N. L. Umedum ◽  
C. B. Nwanya
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Solvent Polarity ◽  
Stem Bark ◽  
Bark Extract ◽  
Root Extract ◽  
Annona Muricata ◽  
Stem Bark Extract

Aim: To compare the phytochemicals and antioxidant activities of stem bark and root extracts of Annona muricata. Methodology: The stem bark and root of Annona muricata were collected, washed, air-dried, ground and each extracted with methanol, ethyl acetate and n-hexane. The extracts were analysed for the presence of phytochemicals. Antioxidant screening was also carried out on the samples. Results: Cardiac glycosides were present in all the extracts of both root and stem bark. Alkaloids were present in moderate abundance in all the extracts except the ethyl acetate stem bark extract. Saponins and tannins were found in methanol extracts of both parts and also in very high abundance but the stem contained higher amount of saponins while alkaloids and tannins were found more in the root. Flavonoids were only found in the ethyl acetate stem bark extract.  Steroids were absent in all the extracts except n-hexane root extract. The root showed greater enzymatic antioxidant activities than the stem bark. The solvent polarity affected the phytochemical found in each extract.  The antioxidant activities of the catalase, superoxidase dismutase and glutathione peroxidase were significantly higher in the root of Annona muricata than in the stem. Conversely, peroxidase showed a significantly higher activity in the stem than in the root. Conclusion: The stem bark and root exhibited good antioxidant properties, so there is need to isolate the compounds responsible for antioxidant property exhibited by the plant parts.

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