Adenine nucleotide metabolism of blood platelets VI. Subcellular localization of nucleotide pools with different functions in the platelet release reaction

1969 ◽  
Vol 186 (2) ◽  
pp. 254-266 ◽  
Author(s):  
Holm Holmsen ◽  
H.James Day ◽  
Eva Storm
Keyword(s):  
Subcellular Localization ◽  
Adenine Nucleotide ◽  
Blood Platelets ◽  
Nucleotide Metabolism ◽  
Release Reaction ◽  
Nucleotide Pools ◽  
Adenine Nucleotide Metabolism ◽  
Platelet Release

Adenine nucleotide metabolism of blood platelets in human liver cirrhosis

1988 ◽  
Vol 51 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Masazumi Zaima ◽  
Masashi Noguchi ◽  
Yasuo Wada ◽  
Keiichiro Mori ◽  
Kazue Ozawa
Keyword(s):  
Liver Cirrhosis ◽  
Human Liver ◽  
Adenine Nucleotide ◽  
Blood Platelets ◽  
Nucleotide Metabolism ◽  
Adenine Nucleotide Metabolism ◽  
Human Liver Cirrhosis

scholarly journals The Effect of Cold on Platelets. III. Adenine Nucleotide Metabolism After Brief Storage at Cold Temperature

Blood ◽  
1973 ◽  
Vol 42 (4) ◽  
pp. 557-564 ◽  
Author(s):  
Herman E. Kattlove ◽  
Dorothy Mormino
Keyword(s):  
Connective Tissue ◽  
Prostaglandin E1 ◽  
Room Temperature ◽  
Adenine Nucleotide ◽  
Platelet Rich Plasma ◽  
Adenine Nucleotides ◽  
Nucleotide Metabolism ◽  
Immunoglobulin M ◽  
Release Reaction ◽  
Adenine Nucleotide Metabolism

Abstract The effect of cold on platelet adenine nucleotide (PAN) metabolism was studied. Spontaneous aggregation which occurs when chilled platelet-rich plasma (PRP) is simultaneously warmed and stirred was not accompanied by the changes in adenine nucleotides associated with the release reaction. Connective tissue caused the release of the same amount of ADP and conversion of equal amounts of ATP to IMP and hypoxanthine in cold-stored platelets as it did in room temperature stored platelets. However, cold did have an important effect on PAN. In PRP stored at cold (0° C, 3° C) temperatures and warmed up to 37° C in the presence of 3H adenine, there was an increase in the conversion of adenine to its metabolites and ultimately to hypoxanthine as compared to PRP stored at warmer temperatures. This effect could not be prevented by ouabain, prostaglandin E1, antibody to immunoglobulin M or adenosine.

scholarly journals The effect of platelet concentrate storage temperature on adenine nucleotide metabolism

Blood ◽  
1975 ◽  
Vol 45 (6) ◽  
pp. 749-756
Author(s):  
DJ Filip ◽  
JD Eckstein ◽  
CA Sibley
Keyword(s):  
Storage Temperature ◽  
Adenine Nucleotide ◽  
Nucleotide Metabolism ◽  
Platelet Concentrate ◽  
Release Reaction ◽  
C Storage ◽  
Adenine Nucleotide Metabolism ◽  
Red Cell Count ◽  
Ph Decrease ◽  
Adenine Nucleotide Pool

The effect of platelet concentrate storage temperature (4 degrees C versus 22 degrees C) on platelet adenine nucleotide metabolism was studied. In general, levels of platelet ATP and ADP, the release reaction, and the metabolis nucleotide pool were best preserved for 72 hr by storage of concentrates at 4 degrees C. Storage of concentrates for 72 hr at 22 degrees C was occasionally associated with a pH decrease to less than 6.0, which is incompatible with platelet viability. When the pH fell below 6.0, there was a marked deterioration of platelet adenine nucleotide levels and the release reaction. The results for concentrates stored at 22 degrees C, with a final pH above 6.0, were not inferior to the results for those stored at 4 degrees C. The pH remained above 7.0 in all concentrates stored at 4 degrees C. The pH changes of platelet concentrates stored at 22 degrees C could not solely be attributed to platelet count, red cell count, or bacterial contamination. Storage at both temperatures was associated with conversion of ATP in the metabolic adenine nucleotide pool to hypoxanthine.

scholarly journals Alterations of Adenine Nucleotide Metabolism and Function of Blood Platelets in Patients With Diabetes

Diabetes ◽  
2007 ◽  
Vol 56 (2) ◽  
pp. 462-467 ◽  
Author(s):  
Anna Michno ◽  
Hanna Bielarczyk ◽  
Tadeusz Pawełczyk ◽  
Agnieszka Jankowska-Kulawy ◽  
Joanna Klimaszewska ◽  
...  
Keyword(s):  
Adenine Nucleotide ◽  
Blood Platelets ◽  
Nucleotide Metabolism ◽  
Adenine Nucleotide Metabolism ◽  
Patients With Diabetes ◽  
And Function

scholarly journals Studies on the mechanism of ristocetin-induced platelet aggregation

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 91-96 ◽  
Author(s):  
HE Kattlove ◽  
MH Gomez
Keyword(s):  
Platelet Aggregation ◽  
Adenine Nucleotide ◽  
Nucleotide Metabolism ◽  
Release Reaction ◽  
Platelet Membranes ◽  
Adenine Nucleotide Metabolism ◽  
Induced Aggregation

Abstract Adenine nucleotide metabolism and the release reaction were studied during ristocetin-induced platelet aggregation. Decreasing platelet ATP by incubation with metabolic poisons did not decrease ristocetin- induced aggregation. ADP and ATP were released from platelets during ristocetin-induced aggregation, and ATP was converted to hypoxanthine. However, these occurred after aggregation was almost complete. Aggregation was inhibited by p-choromercuribenzoic acid. By studying platelet suspensions, we were able to determine that this effect was on platelets and not on the plasma cofactor needed for aggregation. We postulate that ristocetin and its cofactor aggregate platelets by binding platelet membranes and that the platelet plays a passive role in this reaction.

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