Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

The use of a new label for fluoroimmunoassay is described. Lucifer yellow VS is a highly fluorescent vinyl sulphone dye which, under mild conditions, forms covalent bonds with amino and sulphydryl groups but is extremely stable in water. A large Stokes shift (110 nm) and an emission maximum at 540 nm give Lucifer yellow further advantages over the more commonly used labels. The use of the dye as a label has been demonstrated by developing a heterogeneous fluoroimmunoassay for human serum albumin. The fluoroimmunoassay gave comparable results to those obtained using a less specific colorimetric dye-binding assay (r = 0·97, n = 20). The advantages, limitations, and other potential uses of Lucifer yellow are discussed.

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Spectroscopic study on flavonoid–drug interactions: Competitive binding for human serum albumin between three flavonoid compounds and ticagrelor, a new antiplatelet drug

Journal of Luminescence ◽

10.1016/j.jlumin.2015.07.037 ◽

2015 ◽

Vol 168 ◽

pp. 69-76 ◽

Cited By ~ 27

Author(s):

Bing-Mi Liu ◽

Jun Zhang ◽

Chong-Liang Bai ◽

Xin Wang ◽

Xin-Zhi Qiu ◽

...

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Drug Interactions ◽

Spectroscopic Study ◽

Human Serum ◽

Competitive Binding ◽

Antiplatelet Drug ◽

Flavonoid Compounds

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Quantitative Binding Assay for Measuring Specific IgG Antibodies to Alpha-Gal Using the Neoglycoprotein Gal-α-1,3-Gal-β-1,4-Glcnac-Human Serum Albumin

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2014.12.1548 ◽

2015 ◽

Vol 135 (2) ◽

pp. AB188

Author(s):

Alexander J. Schuyler ◽

Hayley R. James ◽

Theo Rispens ◽

Lisa J. Workman ◽

Matthew S. Perzanowski ◽

...

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Binding Assay ◽

Igg Antibodies ◽

Specific Igg ◽

Specific Igg Antibodies

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Antibodies to Testosterone-3-Bovine Serum Albumin, Applied to Assay of Serum 17β-ol Androgens

Clinical Chemistry ◽

10.1093/clinchem/17.7.581 ◽

1971 ◽

Vol 17 (7) ◽

pp. 581-584 ◽

Cited By ~ 72

Author(s):

Jeffrey C Chen ◽

Elinor M Zorn ◽

Marvin C Hallberg ◽

Ralph G Wieland

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Bovine Serum ◽

Binding Assay ◽

Virgin Female ◽

Competitive Binding ◽

Competitive Binding Assay ◽

Menstruating Women ◽

Normal Men ◽

Mean Concentration

Abstract Testosterone was conjugated to bovine serum albumin at carbon 3, and antibodies to this conjugate were developed in virgin female rabbits. This antiserum, diluted 1:3200, was used to measure testosterone. With tritiated testosterone and anti-rabbit γ-globulin as the second antibody, a doseresponse curve was obtained for doses in the range 0.1-10 ng. The antibody cross-reacts extensively with dihydrotestosterone, so that dihydrotestosterone is measured with testosterone in extracted but unchromatographed serum. Results for pooled serum from men, as measured repeatedly by radioimmunoassay and competitive binding assay for testosterone, agreed well. Seventeen normal men had serum 17β-ol androgen concentrations of 667 ng/100 ml (SD, ±248); seventeen normal, menstruating women had a mean concentration of 69 ng/100 ml (SD, ±18). Values for 17β-ol androgens in adolescent males agreed reasonably with testosterone values reported by other investigators. Values are presented for patients with various androgenic disorders. This procedure is easy; many samples can be processed at one time.

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