Antibodies to Testosterone-3-Bovine Serum Albumin, Applied to Assay of Serum 17β-ol Androgens

1971 ◽  
Vol 17 (7) ◽  
pp. 581-584 ◽  
Author(s):  
Jeffrey C Chen ◽  
Elinor M Zorn ◽  
Marvin C Hallberg ◽  
Ralph G Wieland
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Assay ◽  
Virgin Female ◽  
Competitive Binding ◽  
Competitive Binding Assay ◽  
Menstruating Women ◽  
Normal Men ◽  
Mean Concentration

Abstract Testosterone was conjugated to bovine serum albumin at carbon 3, and antibodies to this conjugate were developed in virgin female rabbits. This antiserum, diluted 1:3200, was used to measure testosterone. With tritiated testosterone and anti-rabbit γ-globulin as the second antibody, a doseresponse curve was obtained for doses in the range 0.1-10 ng. The antibody cross-reacts extensively with dihydrotestosterone, so that dihydrotestosterone is measured with testosterone in extracted but unchromatographed serum. Results for pooled serum from men, as measured repeatedly by radioimmunoassay and competitive binding assay for testosterone, agreed well. Seventeen normal men had serum 17β-ol androgen concentrations of 667 ng/100 ml (SD, ±248); seventeen normal, menstruating women had a mean concentration of 69 ng/100 ml (SD, ±18). Values for 17β-ol androgens in adolescent males agreed reasonably with testosterone values reported by other investigators. Values are presented for patients with various androgenic disorders. This procedure is easy; many samples can be processed at one time.

Radioimmunoassay of Serum Estrogen

1973 ◽  
Vol 19 (7) ◽  
pp. 740-747 ◽  
Author(s):  
Nai-Siang Jiang ◽  
Robert J Ryan ◽  
A Albert
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Postmenopausal Women ◽  
Bovine Serum ◽  
Normal Men ◽  
Estradiol 17Β ◽  
Fertile Women

Abstract Radioimmunoassay of serum estrogen (sum of estrone and estradiol-17β) in 0.5 ml of human serum is described. After dilution of the serum, the estrogens are adsorbed on a Sephadex G-15 column in the presence of dilute HCl and then eluted with benzene. After evaporation of the benzene eluate, the estrogens are dissolved in bovine serum albumin (5 g/ liter solution, at pH 8.0). An aliquot of this solution is used for the assay. Serum estrogen concentrations (mean ± SD) were 69 ± 23 pg/ml in 105 normal men and 199 ± 146 pg/ml in 26 fertile women. In 109 postmenopausal women, estrogen was undetectable in seven sera, but in the remaining 102 it was 52 ± 30 pg/ml of serum.

A 125I-radioimmunoassay for measuring androstenedione in serum and in blood-spot samples from neonates.

1989 ◽  
Vol 35 (8) ◽  
pp. 1706-1712 ◽  
Author(s):  
S Thomson ◽  
A M Wallace ◽  
B Cook
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Congenital Adrenal Hyperplasia ◽  
Bovine Serum ◽  
Dried Blood Spot ◽  
Adrenal Hyperplasia ◽  
Steroid Nucleus ◽  
Polycystic Ovarian Disease ◽  
Normal Men ◽  
Blood Spot

Abstract We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking [125I]iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-[125I]iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.

A COMPARISON OF THE COMPETITIVE PROTEIN-BINDING ASSAY AND RADIOIMMUNOASSAY FOR PLASMA PROGESTERONE DURING THE NORMAL MENSTRUAL CYCLE

1972 ◽  
Vol 54 (3) ◽  
pp. 445-456 ◽  
Author(s):  
CHRISTINE A. MORGAN ◽  
I. D. COOKE
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protein Binding ◽  
Bovine Serum ◽  
Binding Assay ◽  
Plasma Samples ◽  
Plasma Progesterone ◽  
Protein Binding Assay ◽  
Competitive Protein Binding ◽  
Free Steroid

SUMMARY A protein-binding assay for plasma progesterone using 2% aqueous chick plasma as the binding solution is described. Details of specifications for petroleum spirit to extract the plasma are given, no chromatographic step being employed. Bound and free steroid are separated by Florisil. The system will assay plasma progesterone at a concentration of 2·0 ng/ml; other reliability data are evaluated. One technician can assay 12 duplicate plasma samples per day. The radioimmunoassay method has utilized two antisera, the first, antiprogesterone-20-0-carboxymethyl-oxime—bovine serum albumin at a dilution of 1 in 3000, the second, anti-progesterone-11-succinyl—bovine serum albumin at a dilution of 1 in 10000. Bound and free steroid are separated by dextran—charcoal suspension. The system will assay plasma progesterone at a concentration of 1·0 ng/ml. One technician can assay 24 duplicate plasma samples per day. There is a good correlation between results obtained by both competitive protein-binding (CPB) and radioimmunoassay (RIA) methods. Both methods have a place in estimating the large numbers of serial samples required in the study of physiological situations, although the RIA method will probably supersede the CPB because of its robustness and greater output.

scholarly journals Competitive Binding of K+ in the Presence of Na+ with Bovine Serum Albumin and Hemoglobin

2016 ◽  
Vol 16 (3) ◽  
pp. 279-284
Author(s):  
S. V. Sidorenko ◽  
◽  
V. G. Rebrov ◽  
D. G. Verkhov ◽  
A. D. Usanov ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Competitive Binding
Sign in / Sign up

Export Citation Format

Share Document