Quantitative Binding Assay for Measuring Specific IgG Antibodies to Alpha-Gal Using the Neoglycoprotein Gal-α-1,3-Gal-β-1,4-Glcnac-Human Serum Albumin

2015 ◽  
Vol 135 (2) ◽  
pp. AB188
Author(s):  
Alexander J. Schuyler ◽  
Hayley R. James ◽  
Theo Rispens ◽  
Lisa J. Workman ◽  
Matthew S. Perzanowski ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Assay ◽  
Igg Antibodies ◽  
Specific Igg ◽  
Specific Igg Antibodies

Use of Lucifer Yellow VS as a Label in Fluorescent Immunoassays Illustrated by the Determination of Albumin in Serum

1983 ◽  
Vol 20 (4) ◽  
pp. 213-216 ◽  
Author(s):  
M Philip Bailey ◽  
Bernard F Rocks ◽  
Clifford Riley
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Assay ◽  
Lucifer Yellow ◽  
Stokes Shift ◽  
Covalent Bonds ◽  
Mild Conditions ◽  
Sulphydryl Groups

The use of a new label for fluoroimmunoassay is described. Lucifer yellow VS is a highly fluorescent vinyl sulphone dye which, under mild conditions, forms covalent bonds with amino and sulphydryl groups but is extremely stable in water. A large Stokes shift (110 nm) and an emission maximum at 540 nm give Lucifer yellow further advantages over the more commonly used labels. The use of the dye as a label has been demonstrated by developing a heterogeneous fluoroimmunoassay for human serum albumin. The fluoroimmunoassay gave comparable results to those obtained using a less specific colorimetric dye-binding assay (r = 0·97, n = 20). The advantages, limitations, and other potential uses of Lucifer yellow are discussed.

Human serum albumin binding assay based on displacement of a non selective fluorescent inhibitor

2007 ◽  
Vol 17 (16) ◽  
pp. 4646-4649 ◽  
Author(s):  
Atli Thorarensen ◽  
Ronald W. Sarver ◽  
Fang Tian ◽  
Andrea Ho ◽  
Donna L. Romero ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Binding Assay ◽  
Albumin Binding ◽  
Human Serum Albumin Binding

IgE and IgG Antibodies to  -Propiolactone and Human Serum Albumin Associated with Urticarial Reactions to Rabies Vaccine

1987 ◽  
Vol 155 (5) ◽  
pp. 909-913 ◽  
Author(s):  
M. C. Swanson ◽  
E. Rosanoff ◽  
M. Gurwith ◽  
M. Deitch ◽  
P. Schnurrenberger ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Rabies Vaccine ◽  
Igg Antibodies

scholarly journals Competitive Protein Binding Assay of Naproxen by Human Serum Albumin Functionalized Silicon Dioxide Nanoparticles

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2593 ◽  
Author(s):  
Qian-Long Wang ◽  
Jing Xie ◽  
Jian Liang ◽  
Geng-Ting Dong ◽  
Li-Sheng Ding ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Silicon Dioxide ◽  
Human Serum ◽  
Binding Assay ◽  
Silicon Dioxide Nanoparticles ◽  
Nano Sio2 ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding

We have developed a new competitive protein binding assay (CPBA) based on human serum albumin functionalized silicon dioxide nanoparticles (nano-SiO2-HSA) that can be used for naproxen determination in urine. Compared with a conventional multi-well reaction plate, nano-SiO2 with a high surface-area-to-volume ratio could be introduced as a stationary phase, markedly improving the analytical performance. Nano-SiO2-HSA and horseradish peroxidase-labeled-naproxen (HRP-naproxen) were prepared for the present CPBA method. In this study, a direct competitive binding to nano-SiO2-HSAwas performed between the free naproxen in the sample and HRP-naproxen. Thus, the catalytic color reactions were investigated on an HRP/3,3′5,5′-tetramethylbenzidine (TMB)/H2O2 system by the HRP-naproxen/nano-SiO2-HSA composite for quantitative measurement via an ultraviolet spectrophotometer. A series of validation experiments indicated that our proposed methods can be applied satisfactorily to the determination of naproxen in urine samples. As a proof of principle, the newly developed nano-CPBA method for the quantification of naproxen in urine can be expected to have the advantages of low costs, fast speed, high accuracy, and relatively simple instrument requirements. Our method could be capable of expanding the analytical applications of nanomaterials and of determining other small-molecule compounds from various biological samples.

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